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Bupleurum chinense hybridization technology overcoming seed production difficulties in high-rainfall region

A kind of hybridization technology, the technology of Bupleurum, which is applied in the field of Bupleurum hybridization technology, can solve the problems of excessive rainfall, castration, bagging and pollination technology can not normally achieve Bupleurum hybridization and seed harvesting, and hinder the promotion of the cultivation of Bupleurum industrialization

Active Publication Date: 2016-11-09
SOUTHWEAT UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

However, Bupleurum is a cross-pollinated plant, and irregular breeding can easily lead to serious germplasm mixing and species degradation, which seriously hinders the industrialization of cultivated Bupleurum.
At the same time, due to the long-term systematic breeding, the breeding of Bupleurum bupleurum for high yield, high efficiency and high adaptability is also facing a bottleneck
At present, the flowering time of Bupleurum in Sichuan is from May to August. During this period, there is a lot of rainfall. Conventional field emasculation, bagging and pollination techniques cannot normally achieve the hybridization and harvesting of Bupleurum.

Method used

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Embodiment Construction

[0016] The Bupleurum hybridization technology for overcoming the difficulty of seed production in high rainfall areas includes the following steps,

[0017] Step 1)—Bupleurum seedling cultivation: Mix peat soil and sandy loam soil at a ratio of 1:1 to prepare a nutrient substrate, remove impurities and impurities from the seeds, and use a 10cm×10cm×10cm nutrient pot to sow seedlings; place the nutrient pot with seeds on a tray Among them, the mode of osmotic watering was used to control the soil moisture; when the seedlings grew to the 3-leaf stage, they were transplanted into 1 gallon culture pots with a diameter of 16 cm, and one seedling was planted in each pot.

[0018] Step 2) - Plant management: Put the transplanted seedlings into an artificial climate chamber for cultivation. The climate chamber uses a Philips plant supplementary sodium lamp (SON-TAGRO 400W), and the placement density of the supplementary sodium lamp is 500umolm when it reaches the leaf surface -2 the s...

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Abstract

The invention relates to a bupleurum chinense intraspecific or interspecific hybridization technology, in particular to a bupleurum chinense hybridization technology overcoming seed production difficulties in a high-rainfall region. Bupleurum chinense is a cross-pollination plant, a serious germplasm mixing and variety degeneration phenomenon is prone to be caused by nonstandard seed reproduction, and meanwhile due to long-term system seed selection, high-yield, efficient and high-adaptability seed selection of the bupleurum chinense is confronted with bottlenecks. In the high-rainfall region, bupleurum chinense hybridization and seed harvesting cannot be normally achieved through the conventional field castration, bagging and pollination technology. The technology comprises the following steps of 1, bupleurum chinense seedling growing; 2, plant management; 3, castration; 4, pollination; 5, seed harvesting. The breeding cycle is greatly shortened, and the problem that bupleurum chinense seed production in the high-rainfall region is difficult is solved; in addition, the technology cannot cause branch breakage caused by too heavy bagging and is widely suitable for intraspecific and interspecific hybridization of the bupleurum chinense varieties including bupleurum chinense, bupleurum scorzonerifolium, bupleurum marginatum and bupleurum falcatum.

Description

technical field [0001] The invention relates to an intraspecific or interspecific hybridization technique of Bupleuri, in particular to a hybridization technique of Bupleuri which overcomes the difficulty in seed production in areas with high rainfall. Background technique [0002] Radix Bupleuri (Radix Bupleuri) is a commonly used bulk medicinal material in my country. It is listed as the top grade in "Shen Nong's Herbal Classic". It has a medicinal history of more than 2,000 years. Fever, alternating cold and heat, poor liver qi, distending pain in the chest and hypochondriac, irregular menstruation, uterine prolapse, etc. It is the main drug and auxiliary drug of more than 70 classic famous prescriptions and Chinese patent medicines. Its medicinal material consumption ranks among the top five in the country, with an annual demand gap of more than 6,000 tons. It is a national strategic emerging industry and a "7+3" key support industry and product in Sichuan Province. Ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G9/10
CPCA01G22/00A01G24/00
Inventor 余马侯大斌刘静波舒晓燕黄晶刘丹刘宏伟段莎莎
Owner SOUTHWEAT UNIV OF SCI & TECH
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