Preparation method of anti-EV-71 virus effective parts after compatibility of herba agastaches and grubs

Abstract

The invention belongs to a traditional Chinese medicine active ingredient extracting process and separation of anti-virus effective parts and mainly relates to a separation method of anti-EV-71 virus effective parts after compatibility of herba agastaches and grub extracts. The separation method comprises the specific steps: refluxing and extracting the herba agastaches and grubs by adopting distilled water, carrying out alcohol precipitation, resisting viruses, dissolving and precipitating, separating the grub extract by adopting D101 type macroporous adsorption resin, wherein the separation effect is relatively good, and determining the anti-virus effective parts; then carrying out compatibility on the effective parts of the two medicines according to the ratios of 9 to 1, 8 to 2, 7 to 3, 6 to 4, 5 to 5, 4 to 6, 3 to 7, 2 to 8 and 1 to 9 respectively, and carrying out anti-EV-71 virus researches again. An anti-virus tracking method finds out that after the compatibility of the anti-virus effective parts of the herba agastaches and the grubs, the anti-virus effect is relatively good and the optimal compatibility ratio is found.

Description

technical field

[0001] The present invention relates to a production and extraction process of active ingredients of traditional Chinese medicine, a new idea, a new method for extracting antiviral effective parts of Huoxiang and grub, and a new application in the field of pharmacy. The active ingredients of syncytial virus, and the antiviral research after the active ingredients are compatible. Background technique

[0002] In recent years, various viruses have suddenly invaded, such as SARS virus, A-H1N1, etc. Every virus outbreak will cause human panic or even death, so it is very important to find suitable antiviral drugs.

[0003] Patchouli is a plant of the genus Patchouli in the family Lamiaceae. It is native to Southeast Asia, such as Malaysia, the Philippines, Indonesia and other countries. my country has a long history of introduction and cultivation, which can be traced back to Liang Dynasty or before.

[0004] Enteroviruses belong to the enterovirus group in Parv...

Examples

example 1

[0019] Example 1 (1) Weigh a certain amount of Huoxiang and grub, respectively add 10 times the amount, heat and reflux to extract twice, the first time is 2 hours, and the second time is 1.5 hours, combine the two extracts, concentrate under reduced pressure to the concentration of each milliliter of medicine The liquid contains 1 gram of medicinal materials, adding ethanol to adjust the concentration of ethanol in the solution to be 70%, and standing for 24 hours.

[0020] (2) Filter the alcohol precipitation solution obtained in (1) under reduced pressure to obtain a precipitate, and dissolve the precipitate with distilled water sonication.

[0021] (3) Apply the solution obtained in (2) to the treated D101, HPD100, DM130, X-5, AB-8 macroporous adsorption resin, rinse with water, take one bottle per 200ml, and rinse until colorless.

[0022] (4) Merge the parts eluted with middle water in (3), concentrate to a certain volume, and carry out antiviral research separately.

...

technical field

[0001] The present invention relates to a production and extraction process of active ingredients of traditional Chinese medicine, a new idea, a new method for extracting antiviral effective parts of Huoxiang and grub, and a new application in the field of pharmacy. The active ingredients of syncytial virus, and the antiviral research after the active ingredients are compatible. Background technique

[0002] In recent years, various viruses have suddenly invaded, such as SARS virus, A-H1N1, etc. Every virus outbreak will cause human panic or even death, so it is very important to find suitable antiviral drugs.

[0003] Patchouli is a plant of the genus Patchouli in the family Lamiaceae. It is native to Southeast Asia, such as Malaysia, the Philippines, Indonesia and other countries. my country has a long history of introduction and cultivation, which can be traced back to Liang Dynasty or before.

[0004] Enteroviruses belong to the enterovirus group in Parv...

Examples

example 1

[0019] Example 1 (1) Weigh a certain amount of Huoxiang and grub, respectively add 10 times the amount, heat and reflux to extract twice, the first time is 2 hours, and the second time is 1.5 hours, combine the two extracts, concentrate under reduced pressure to the concentration of each milliliter of medicine The liquid contains 1 gram of medicinal materials, adding ethanol to adjust the concentration of ethanol in the solution to be 70%, and standing for 24 hours.

[0020] (2) Filter the alcohol precipitation solution obtained in (1) under reduced pressure to obtain a precipitate, and dissolve the precipitate with distilled water sonication.

[0021] (3) Apply the solution obtained in (2) to the treated D101, HPD100, DM130, X-5, AB-8 macroporous adsorption resin, rinse with water, take one bottle per 200ml, and rinse until colorless.

[0022] (4) Merge the parts eluted with middle water in (3), concentrate to a certain volume, and carry out antiviral research separately.

...