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Method for detecting sulbactam in milk

A detection method, milk technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., and can solve the problems of antibiotics and β-lactamase detection methods that need to be studied.

Inactive Publication Date: 2016-11-23
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current detection methods for antibiotics and β-lactamase in milk still need to be studied

Method used

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  • Method for detecting sulbactam in milk
  • Method for detecting sulbactam in milk
  • Method for detecting sulbactam in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] In this example, sulbactam in milk was detected according to the following method:

[0034] 1. Reagents and solutions

[0035] Spanish ECLIPSE50 (referred to as E50) antibiotic detection kit, penicillin sodium standard, β-lactamase standard (produced by China Institute for the Control of Pharmaceutical and Biological Products, the concentration is 4.46 million U / mL), sulbactam standard,

[0036] Reconstituted milk: obtained by dissolving Fonterra milk powder in water, free of antibiotics, beta-lactamase and sulbactam.

[0037] 2. Equipment and instruments

[0038] Vortex mixer, constant temperature water bath (40°C±1°C), incubator (65°C±1°C) required for antibiotic detection, pipette (10uL, 100uL and 1mL), centrifuge tubes (1.5mL, 2mL and 15mL ).

[0039] 3. Operation steps

[0040](1) Take 1 mL of reconstituted milk, add 6 μL of 1 mg / L penicillin sodium solution to a final concentration of 6 μg / L, vortex and mix well, and use it as the antibiotic positive control g...

Embodiment 2

[0051] accuracy check

[0052] Specific operation:

[0053] (1) Take three New Zealand reconstituted milk and add 1mg / L, 5mg / L and 10mg / L sulbactam respectively to obtain spiked sample 1, spiked sample 2 and spiked sample 3,

[0054] Take two parts of reconstituted milk, add 10mg / L and 100mg / L sulbactam respectively, to obtain spiked sample 4 and spiked sample 5;

[0055] (2) Carry out 2 parallel detections of sulbactam in reconstituted milk and spiked samples 1-3 according to the method of Example 1 respectively;

[0056] (3) Carry out 2 parallel detections of sulbactam in the spiked samples 4 and 5 according to the method of Example 1 respectively, the difference is that without steps (1) to (3), the spiked samples 4 and 5 are directly Incubation treatment.

[0057] The results are shown in Table 2. From spiked samples 1-3, it can be seen that when the concentration of sulbactam is 1 mg / L, β-lactamase is not completely inhibited, and the result is false negative, and sul...

Embodiment 3

[0063] Stability verification

[0064] Specific steps:

[0065] (1) Select 20 different batches of milk samples without sulbactam, add 10 mg / L sulbactam to each of the 10 different batches of milk, and record them as samples 1-10; the other 10 different batches The second milk without sulbactam is recorded as samples 11-20;

[0066] (2) According to the method of Example 1, the sulbactam in samples 1-20 were detected respectively.

[0067] The results are shown in Table 3. It can be seen that the method of the present invention can accurately detect whether the sulbactam exists, and the stability is good.

[0068] Table 3 Stability Verification

[0069]

[0070] The solutions of the present invention will be explained below in conjunction with examples. Those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be considered as limiting the scope of the present invention. If no specific technique...

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Abstract

The invention discloses a detection method for sulbactam in milk. The method comprises: (1) detecting the antibiotics in the antibiotic positive control group, the β-lactamase positive control group and the sample group respectively, wherein, the antibiotic positive control group is obtained by incorporating antibiotic-free, β-lactamase Amase and sulbactam milk mixed with antibiotics obtained; the β-lactamase positive control group is obtained by mixing milk without antibiotics, β-lactamase and sulbactam, antibiotics and β-lactam Amidase is obtained by mixing; the sample group is obtained by mixing the milk to be tested, antibiotics and β-lactamase; (2) based on the detection results, the sulbactam in the milk to be tested is qualitatively determination. The method of the invention can accurately detect the sulbactam in milk and is easy to operate.

Description

technical field [0001] The invention relates to the field of food. Specifically, the present invention relates to a detection method for sulbactam in milk. Background technique [0002] With the development of dairy cattle breeding, antibiotics have been widely used in the prevention and treatment of dairy cattle diseases. However, the antibiotics in cows will flow into the milk along with the mammary glands, and the antibiotics left in the milk will have adverse effects on human health. Therefore, at present, my country has clearly stipulated the limit of antibiotic residues in milk. However, some unscrupulous merchants take advantage of the feature that β-lactamase can decompose antibiotics, and add β-lactamase to milk to cover up and condone the abuse of antibiotics. In addition, after eating milk containing β-lactamase, it will cause Antibiotics ingested by humans are less effective and cannot achieve the effect of treating diseases. Therefore, β-lactamase is clearly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 解鑫喻东威赵媛梁春梅袁凤琴李照吴腾李秋琴宋晓东
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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