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Method for obtaining plant-type-improved new rice breeding materials without selectable markers

A technology without selection markers and plant type improvement, which is applied in the field of obtaining new materials for rice breeding, can solve the problems of slow speed and difficult realization, and achieve the effect of increasing crop yield

Inactive Publication Date: 2016-12-14
CHINA NAT RICE RES INST
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Problems solved by technology

[0004] The object of the present invention is to provide a method for obtaining new rice breeding materials for plant type improvement without selection markers, aiming to solve the problem that the traditional cross breeding improvement of plant type is not only slow but also difficult to achieve

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  • Method for obtaining plant-type-improved new rice breeding materials without selectable markers

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Embodiment Construction

[0013] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0014] The present invention utilizes the combination of the promoter of the CYP734A4 gene itself and the 35S enhancer to drive the overexpression of the CYP734A4 gene, so that not only normal fruiting can be obtained, but also a new plant type transgenic rice with erect leaves can be obtained.

[0015] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0016] Such as figure 1 As shown, the method for obtaining new rice breeding materials without selection markers in the embodiments of the present invention includes the following steps:

[...

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Abstract

The invention discloses a method for obtaining plant-type-improved new rice breeding materials without selectable markers. The method comprises the following steps: constructing an amplification enhanced subsequence which uses pCambia1300 vector as a template; using a Giboson Assemble fragment assembling kit to connect four recovered fragments into a target vector p35SBRD3; using the vector p35SBRD3 for rice transforming. The method provided by the invention can realize the rapid polymerization of genes through transgenosis; however, the presently available genes are extremely limited; compared with OsDWARF4 gene and OsBRI1 gene reported at present, BRD3 gene is involved in the degradation pathway of BR metabolism so as to more easily realize the fine adjustment of BR at different parts; the expression levels of the BRD3 gene at different parts can be finely adjusted by transgenic technology, so that the ideal plant type can be shaped and the yield of crops can be increased.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a method for obtaining new rice breeding materials with improved plant type without selection markers. Background technique [0002] Rice is the food crop with the largest sown area and the highest total output in my country. Its sown area and output account for more than 1 / 3 of the food crops. Nearly 2 / 3 of the country's population takes rice as a staple food. It can be said that rice production has been stabilized. , To a large extent, it has stabilized the grain supply of our country. In recent years, with the development of my country's economy and the acceleration of urbanization, the area of ​​rice planting has dropped significantly, which directly leads to the continuous decline of my country's total rice production and rice stocks. At present, my country's food supply and demand are in a "tight balance" state. With the continuous growth of my country's p...

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Application Information

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IPC IPC(8): C12N15/84A01H5/00
CPCC12N15/8205C12N15/8261C12N15/8298C12N2800/60
Inventor 刘文真付亚萍胡国成
Owner CHINA NAT RICE RES INST
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