Lagotis brevituba extract, preparation methods and application thereof, and externally-applied extract-containing agent for skins
A technology of short-tube rabbit ear grass and skin external preparation, which can be applied in the directions of skin care preparations, skin diseases, drug combinations, etc., can solve the problems of poor anti-aging and whitening effect, low safety, etc. Effect
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Embodiment 1
[0036] Embodiment 1 is that the ethanol aqueous solution of 50% is extraction solvent (method 1) with volume concentration
[0037] 20g of dry powder of the short-tube rabbit's ear grass (the particle size of the short-tube rabbit's ear grass powder is 20 meshes), at room temperature (25°C) with 200ml of ethanol aqueous solution with a volume concentration of 50%, ultrasonic extraction 2 times, each time for 30min (power 500W), take the filtrate and combine, depressurize (50 ℃) to recover the solvent to dryness, take an appropriate amount of extract, add DMSO (dimethyl sulfoxide) to prepare a 0.05mg / ml solution for later use, measure its free radical scavenging effect, its free Base clearance was 15%.
Embodiment 2
[0038] Embodiment 2 is that the ethanol aqueous solution of 60% is extraction solvent (method 1) with volume concentration
[0039] 20g of dry powder of the short-tube rabbit's ear grass (the particle size of the short-tube rabbit's ear grass powder is 30 mesh), at room temperature (25°C) with 200ml of ethanol aqueous solution with a volume concentration of 60%, ultrasonic extraction twice, each time for 30min (power 500W ), take the filtrates and combine them, recover the solvent under reduced pressure (50°C) to dryness, take an appropriate amount of extract, add DMSO (dimethyl sulfoxide) to prepare a 0.05mg / ml solution for later use, measure its free radical scavenging effect, and its free radical scavenging The rate is 17%.
Embodiment 3
[0040] Embodiment 3 is that the ethanol aqueous solution of 70% is extraction solvent (method 1) with volume concentration
[0041] 20g of dry powder of the short-tube rabbit's ear grass (the particle size of the short-tube rabbit's ear grass powder is 40 mesh), at room temperature (25°C) with 200ml of ethanol aqueous solution with a volume concentration of 70%, ultrasonic extraction twice, each time for 30min (power 500W ), take the filtrates and combine them, recover the solvent under reduced pressure (50°C) to dryness, take an appropriate amount of extract, add DMSO (dimethyl sulfoxide) to prepare a 0.05mg / ml solution for later use, measure its free radical scavenging effect, and its free radical scavenging The rate is 48%.
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