Anticancer composition containing paeoniae rubra radix (PRR) and astragalus root, and preparation method and application thereof
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An anti-tumor drug and anti-tumor technology, applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc.
Active Publication Date: 2017-02-01
GANSU UNIV OF CHINESE MEDICINE
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[0004] However, there is still a need to further impro
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Embodiment 1
[0032] Example 1 Preparation of Radix Paeoniae Rubra Extract
[0033] Take 1Kg of Radix Paeoniae Rubra, add 10Kg of water to soak for 1h, decoct 3 times, each time for 1h, decoct the filtrate and add 10Kg of water each time, after combining the decoction, use 20% of the decoction weight of petroleum ether, acetic acid in turn Ethyl ester and n-butanol extraction, the extracts obtained in the three steps were distilled under reduced pressure to remove the solvent, and then dried to obtain the extract.
Embodiment 2
[0034] Example 2 Preparation of antitumor composition
[0035] Prepare the composition according to the weight in the following table, and after mixing evenly, the composition is obtained.
[0036] .
Embodiment 3
[0046] Embodiment 3 drug effect test
[0047] Escherichia coli OP50 culture
[0048] Inoculate the OP50 purchased from the Nematode Genetics Center and cultivated on solid medium with an inoculation loop into LB liquid medium, put it on a shaker, culture conditions 200rpm, 20°C, cultivate for 24h, part of it is used to smear bacteria, each plate Add 400 μl of bacterial solution, spread it evenly with a smear stick, place it in a constant temperature incubator for 24 hours, and use it for nematode transfer; the other part is centrifuged (10000rpm, 10min) to obtain solid bacteria, weigh it, and then dilute it to 110mg / ml The bacterial solution was stored in a refrigerator at 4°C.
[0049] nematode culture
[0050] Caenorhabditis elegans MT2124 frozen in a -80°C refrigerator was transferred to solid NGM medium coated with OP50, and adult worms were cultured at 20°C for synchronization. The synchronized nematodes were hatched into small nematodes in the L1 stage for biologi...
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Abstract
The invention relates to an anticancer composition containing paeoniae rubra radix (PRR) and astragalus root, and a preparation method and application thereof and belongs to the technical field of traditional Chinese medicines. The composition comprises the following components in percentage by weight: 80-94 percent of paeoniae rubra radix extract, 1-18 percent of astragalus polysaccharide and 0.1-5 percent of panax japonicus. The invention provides a composition containing the paeoniae rubra radix extract and the astragalus polysaccharide; the effect of the paeoniae rubra radix extract on inhibiting the excessive activation of a proto-oncogene ras pathway can be obviously improved through the synergistic effect of the astragalus polysaccharide; the toxicity of the composition is reduced through the panax japonicus. The composition has obvious inhabiting and regulating effects on Ras genes.
Description
technical field [0001] The invention relates to an antitumor composition containing Radix Paeoniae Rubra and Radix Astragali, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. Background technique [0002] Paeoniae Rubra Paeoniae Rubra Radix (PRR) is the Paeoniae officinalis of the Ranunculaceae (Ranunculaceae) plant Paeonia lactiflora Pall. or Radix Paeoniae Rubra Paeonia veitchii Lynch dry roots. It is bitter in taste, slightly cold in nature, and returns to the liver meridian. It has the effects of clearing away heat and cooling blood, dispelling stasis and relieving pain. [1] . Modern studies have shown that Radix Paeoniae Rubra contains various chemical components such as terpenoids and their glycosides, flavonoids and their glycosides, and volatile oils, and has liver protection, anti-tumor (Wu Yangzheng, etc.), neuroprotection, heart protection, anti-thrombotic, and anti-inflammatory effects. ...
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