Lithocarpus amygdalifolius tissue culture explant obtaining method

A technology of tissue culture and acquisition method, which is applied in botany equipment and methods, horticultural methods, cultivation, etc. It can solve the problems that it is difficult for the sprouts of big trees to meet the actual production and seedling needs, it is difficult to become a common material for tissue culture, and it is inconvenient to pick. Achieve the effects of excellent explant quality, convenient mother plant management and shoot collection, and improved survival rate

Inactive Publication Date: 2017-02-22
LIUZHOU SANNONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the large trees are located in remote forest areas, which are inconvenient to pick, difficult to germinate, small in number and difficult to sterilize. Therefore, it is difficult for large tree sprouts to meet the actual production and seedling needs.
However, isolated embryos, sterile seedlings, cotyledons and needles are used as explant materials, and the speed and quantity of adventitious buds obtained are not as fast and as many as stem segments, so it is difficult to become common materials for tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Select the seeds from the good family of Xingye Ke that are free from pests and diseases and full of particles. After conventional disinfection and hot water soaking, the seeds are dried in an oven at 100-140℃, and they are naturally cooled to room temperature and sown at a volume concentration of 0.3%. The potassium permanganate solution disinfected the sand bed.

[0027] After the sprouts are unearthed, when the height of the sprouts is 6-8 cm, transplant them into a 15cm×15cm non-woven nursery bag, water and spray as appropriate, and apply a 0.5% compound concentration every 7-10 days. Fertilizer solution, in which the effective component of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root stable after 40 days.

[0028] When the transplanted seedlings have stable roots, select the container seedlings with a height of 40-60 cm, a ground diameter of 0.3-0.6 cm, a straight trunk and few lateral branches as the mother plant, and transplant them ...

Embodiment 2

[0031] Select the seeds from the good family of Xingye Ke that are free from pests and diseases and full of particles. After conventional disinfection and hot water soaking, the seeds are dried in an oven at 110°C. After natural cooling to room temperature, the seeds are sown at a high volume concentration of 0.3%. Sand bed disinfected with potassium manganate solution.

[0032] After the sprouts are unearthed, when the height of the sprouts is 6-8 cm, they are transplanted into a 15cm×15cm non-woven seedling bag, water and spray as appropriate, and a 0.5% compound fertilizer solution with a mass concentration of 0.5% is applied every 8 days , The effective component of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root stable after 40 days.

[0033] When the transplanted seedlings are stable and rooted, select the container seedlings with a height of 45-50 cm, a ground diameter of 0.3-0.4 cm, a straight trunk and few lateral branches as the mother plan...

Embodiment 3

[0036] Select the seeds from the good family of Xingye Ke that are free from pests and diseases and full of particles. After conventional disinfection and hot water soaking, the seeds are dried in an oven at 120°C. After they are naturally cooled to room temperature, the seeds are sown in a high volume concentration of 0.3%. Sand bed disinfected with potassium manganate solution.

[0037] After the sprouts are unearthed, when the height of the sprouts is 6~8 cm, transplant them into a 15cm×15cm non-woven nursery bag, water and spray as appropriate, and apply 0.5% compound fertilizer solution with mass concentration every 9 days , The effective component of NPK in the compound fertilizer solution is ≥48%, and the sprouts will take root stable after 40 days.

[0038] When the transplanted seedlings are rooted and stable, select the container seedlings with a height of 50-55 cm, a ground diameter of 0.4-0.5 cm, a straight trunk and few lateral branches as the mother plant, and transpl...

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PUM

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Abstract

The invention discloses a lithocarpus amygdalifolius tissue culture explant obtaining method. The method includes the procedures of seed selection and sowing, seedling transplanting and management, mother plant planting and management, and explant obtaining. Mother plant planting is conducted after selection of good seed sources and selection of good strong seedlings, and proper sprouts are cut from mother plants, and then sterilization and inoculation are conducted. Mother plant management and sprout collection are very convenient, an obtained explant is good in quality, the pollution rate and the death rate are low, the adventitious bud germination rate is high, sprouts are regular, operability is high, the requirement for building a ithocarpus amygdalifolius tissue culture rapid propagation system is met, and economic benefits, social benefits and ecological benefits are high.

Description

Technical field [0001] The invention belongs to the technical field of plant vegetative propagation, and relates to a method for obtaining tissue culture explants of Cole spp. Background technique [0002] Lithocarpus amygdalifolius (Skan) Hayata, also known as Lithocarpus amygdalifolius (Skan) Hayata, is a tree of the Fagaceae genus (Lithocarpus). The new branches and young leaves are densely covered in spring and summer. Brown curly pilose, all hairs fall off after autumn. The male spikes are axillary or multiple spikes arranged in panicles, the inflorescences are densely pilose; the female flowers are in clusters of 3, sometimes with single scattered. The shell is nearly spherical, full of nuts, in a discontinuous ring shape; the fruit wall of the nut is slightly thicker than the shell, and the top of the nut is puberulent. Distributed in central and southern Taiwan, southern Fujian, areas south of the Tropic of Cancer in southeastern Guangdong, and Hainan. Born in evergree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01H4/00
CPCA01G17/005A01H4/00
Inventor 陈杏林
Owner LIUZHOU SANNONG TECH
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