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Tissue culture method of gesneriaceae plant

A technology of Gesneriaceae and tissue culture, applied in the field of bioengineering, can solve problems such as tissue culture, and achieve the effects of simple operation, less labor, and good method versatility

Inactive Publication Date: 2017-02-22
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no effective method for tissue culture

Method used

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  • Tissue culture method of gesneriaceae plant

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Embodiment Construction

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] like figure 1 A method for tissue culture of a Gesneriaceae plant provided by the present invention comprises the following steps,

[0022] Step S1: punching the isolated leaves of Gesneriaceae plants to obtain leaf discs, which specifically includes step (1): under sterile conditions, select the leaves of test-tube plantlets with a seedling age of 8-10 weeks; step (2): The detached leaves of the Gesneriaceae plant were punched through a sterile punche...

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Abstract

The invention relates to a tissue culture method of a gesneriaceae plant. The method comprises the following steps: punching in-vitro leaves of the gesneriaceae plant to obtain leaf discs; transferring the obtained leaf discs into an adventitious bud inducing medium; cutting induced adventitious buds on the leaf discs, and transferring the adventitious buds into a normal MS medium in order to produce single rooted seedlings of the gesneriaceae plant. The method increases the quantity of the adventitious buds induced by a single leaf disc, improves the breeding efficiency of tissue culture seedlings, has the characteristics of simplicity in operation, less labor and small labor intensity, large inoculation amount and very good versatility, and lays a solid foundation for large-scale rapid propagation of gesneriaceae plants, especially Chirita dielsii, Chirita speciosa, Lysionotus pauciflorus, Lysionotus serratus and Lysionotus sulphureoides.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for tissue culture of Gesneriaceae plants. Background technique [0002] Gesneriaceae plants are mostly herbs with rhizomes, and a few are shrubs or trees. Leaves opposite or basal, sparse whorled or alternate, usually simple, undivided, sparsely pinnatifid or pinnately compound. Flowers usually in cymes, bisexual, actinomorphic or bilaterally symmetrical. Sepals 4-5 pieces. Corolla campanulate or radial, mostly bilabiate, stamens all fertile, or 4 or 2 fertile; pistil formed by 2 carpels, ovary superior, semi-inferior or completely inferior, 1-loculed, or 2-loculed, Ovules numerous. Capsule, dorsally or interlocularly longitudinally lobed, sparsely capped (Scutellaria) or berry. There are about 135 genera and 2000 species, distributed in the tropics and subtropics, and a few in the temperate zone. There are about 413 species in 56 genera in China, from sout...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李唯奇贾艳霞林亮袁彬
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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