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A kind of Bacillus brevis strain and its application

A technology of Bacillus brevis and strains, applied in the field of microorganisms, can solve the problems of chemical control of polluted environment, strains of Bacillus brevis, unsafe, etc., and achieve the effect of high control effect, good control effect and huge application potential.

Active Publication Date: 2019-05-17
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of Bacillus brevis strain and its application in order to solve the pollution environment and unsafe problems existing in chemical control

Method used

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  • A kind of Bacillus brevis strain and its application
  • A kind of Bacillus brevis strain and its application
  • A kind of Bacillus brevis strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation, purification and identification of Bacillus brevis:

[0024] Take soil samples evenly from the tomato planting fields in Shunyi, mix 10g of soil with 100mL of sterilized water to make a suspension, and then carry out gradient dilution in turn, take 10000 times diluted soil mixture 100μL and spread it on the LB medium plate, The formula of LB medium is: yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar powder 1.3-1.5g / 100mL, dilute to 1L and seal. After sterilizing at 121° C. for 20 minutes at high temperature and high pressure, spread it into a flat plate in a petri dish. The medium was cultured at 37°C. After the tissue pieces grew colonies, a single colony was picked and purified on LB medium for classification and identification. The strains were morphologically determined according to the Bergey's Manual of Bacterial Identification.

[0025] Pick a single colony with a sterilized toothpick, put it into an EP tube filled with 300 μL of bacterial lysate ...

Embodiment 2

[0030] 1, the preparation method of Bb-1 bacterial strain fermented liquid, comprises the following steps:

[0031] (1) Streak-inoculate Bacillus brevus Bb-1 on LB medium, culture it on a plate in a constant temperature incubator for 60 hours, set the culture temperature at 37°C, and obtain a single colony;

[0032] (2) Inoculate the single colony of Bacillus brevus Bb-1 in the seed culture medium (LB medium), shake and cultivate in a constant temperature shaking table, the rotating speed is 160rpm, the temperature is 37°C, and shake and cultivate for 36h; liquid seed culture medium The formula is LB medium, that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, and the volume is adjusted to 1L;

[0033] (3) Inoculate the seed liquid into the sterilized fermentation medium for fermentation culture, the inoculum size is 1%, the temperature is set at 37°C, and the obtained concentration is 10 6 cfu / mL fermentation broth (stock solution); liquid fermentation medium formula is LB...

Embodiment 3

[0045] 1, the preparation method of Bb-1 bacterial strain fermented liquid, comprises the following steps:

[0046] (1) Streak-inoculate Bacillus brevus Bb-1 on LB medium, and culture it on a plate in a constant temperature incubator for 60 hours at a temperature of 37°C to obtain a single colony;

[0047] (2) Inoculate a single colony of Bacillus brevus Bb-1 in the seed medium, and cultivate it in a constant temperature shaker with a rotation speed of 160rpm and a temperature of 37°C for 36 hours; the formula of the liquid seed medium is LB medium , that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, dilute to 1L;

[0048] (3) Inoculate the seed liquid into the sterilized fermentation medium for fermentation culture, the inoculum size is 1%, the temperature is set at 37°C, and the obtained concentration is 10 6 cfu / mL fermentation broth; liquid fermentation medium formula is LB medium, that is, yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, and the volume is adjusted to 1L...

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Abstract

The invention discloses a strain of Bacillus brevis and an application thereof. The strain is Bacillus brevis Bb-1, which is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC NO.12365. Application of biocontrol agent prepared by Bacillus brevis Bb‑1 in the control of root-knot nematode. The Bacillus brevis in the present invention is isolated from the soil, has good control effect on root-knot nematode, is easy to colonize in soil, and is beneficial to the early prevention and control of root-knot nematode damage. The application potential of this method is helpful to realize high-yield, safe and pollution-free production of vegetables; the lethality rate of the fermentation liquid of Bacillus brevis Bb‑1 strain to root-knot nematodes reaches 100%, and the control effect in pot experiments reaches 81.6% , high and stable control effect, suitable for agricultural production needs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus brevis and an application thereof. Background technique [0002] Root-knot nematode (Meloidogyne) is a highly specialized omnivorous plant pathogenic nematode. Nematodes known to harm vegetables mainly include root-knot nematode peanut, root-knot nematode northern, root-knot nematode southern, root-knot nematode java, and root-knot nematode beet. Nematodes have a wide range of hosts, often harming more than 30 kinds of vegetables such as melons, solanaceous fruits, beans, radishes, carrots, lettuce, and cabbage, and can also spread some fungal and bacterial diseases. Root-knot nematode is an important crop pathogen, causing losses of about billions of dollars every year. Root-knot nematodes mainly infect the roots of crops. After infection, the roots swell to form huge root knots, which can be connected to form beads. Because the roots ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 谢丙炎茆振川杨宇红凌键张晓平
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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