Construction method for normal and whitening skin cell line of bastard halibut

A technology of skin cells and construction methods, applied in artificial cell constructs, epidermal cells/skin cells, biochemical equipment and methods, etc., can solve problems affecting the market value of flounder fry, individual survival ability, and retention, etc., and achieve chromosome The identification method is reliable, the repeatability is strong, and the effect of appropriate weight

Pending Publication Date: 2017-03-15
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This bleaching has seriously affected the market value and individual viability of flounder fry
So far, the research on the albino mechanism of flou...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method for normal and whitening skin cell line of bastard halibut
  • Construction method for normal and whitening skin cell line of bastard halibut
  • Construction method for normal and whitening skin cell line of bastard halibut

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] In order to enable those skilled in the art to better understand the present invention, the construction, identification and application methods of the normal and albino skin cell lines of flounder flounder of the present invention will be described in detail below in conjunction with the accompanying drawings and examples.

[0026] 1. Construction method of normal skin cell line and albino skin cell line of flounder

[0027] A method for constructing normal and albino skin cell lines of flounder, comprising three steps:

[0028] Step 1: Prepare cell culture medium: Weigh 9.6 grams of DMEM / F12 medium and 4.76 grams of Hepes respectively, fully dissolve and mix for 4 hours, adjust the pH value to 7.4 with NaOH, then filter with suction, divide the filtrate, and place in Store at 4°C. When in use, take the filtrate and add fetal bovine serum accounting for 20% of the total volume of DMEM / F12 medium, 50mM 2-mercaptoethanol, 10ng / ml human basic fibroblast growth facto...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of marine organism cell culture and specifically relates to a construction method for a normal and whitening skin cell line of bastard halibut. The method comprises the following steps: firstly preparing a cell culture fluid, taking normal and whitening skin tissues of the bastard halibut as materials, rinsing and cutting, then directly putting into a 25cm<2> culture flask filled with a defined amount of cell culture fluid, and culturing at 24 DEG C; exchanging the cell culture fluid every 5 days, and adopting 0.25% trypsin for digesting cells for sub-culturing when the original generation cell grows into a single layer; and sub-culturing for one time per 8-10 days, and successfully constructing a cell line when the tenth generation is sub-cultured and the serum content in the cell culture fluid is reduced from 20% to 10%. The cell line of bastard halibut, acquired according to the method, is in a fiber form, more than 40 generations can be continuously sub-cultured, and the cell line can be directly applied to the etiologic characteristic research, vaccine research and functional gene research. The construction method is suitable for constructing the skin cell lines of the other fishes.

Description

technical field [0001] The invention belongs to the technical field of marine biological cell culture, and in particular relates to a method for constructing normal and albino skin cell lines of flounder. Background technique [0002] Since the first teleost cell line—the rainbow trout gonad cell line RTG-2 (Wolf and Quimby, Science, 1962, 135:1065-1066) was successfully constructed in 1962, nearly 300 different fish cell lines have been developed successively Established, tissue sources include embryonic and adult fish kidneys, hearts, spleens, fin rays, gonads, etc. The application of these cell lines has also been extended from virus isolation and identification to research in the fields of fish embryonic stem cells, immunology, etiology, genetic breeding and genetic engineering, and fish resource protection. However, fish skin is an important pathogen defense barrier and a tissue for pigmentation. So far, only a few fish have skin cell lines (Lei et al., Fish Physiology...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/071
CPCC12N5/0625C12N2500/44C12N2501/115
Inventor 王娜王若青张念伟王仁凯陈松林
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap