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A kind of endothelial cell antigen related to transplant rejection and its application and kit

A technology of transplant rejection and reagents, which can be used in cytokeratin, biological testing, peptide sources, etc., and can solve the problem of unclear antigen molecules.

Active Publication Date: 2020-02-14
CENT SOUTH UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

Exactly which antigenic molecules on the surface of endothelial cells are targeted by anti-endothelial cell antibodies is unclear

Method used

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  • A kind of endothelial cell antigen related to transplant rejection and its application and kit
  • A kind of endothelial cell antigen related to transplant rejection and its application and kit
  • A kind of endothelial cell antigen related to transplant rejection and its application and kit

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Experimental program
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Effect test

Embodiment

[0026] Reagent source:

[0027] M199 medium, vascular endothelial growth factor, collagenase, fetal bovine serum, and protein A microspheres are commercial products purchased from Invitrogen; mixed healthy human platelet reagents are purchased from Sigma; HLA antibody detection kits are purchased from One Lambda The company; MICA antibody detection reagent; protein mass spectrometry identification is completed by BGI.

[0028] Screening of clinical samples

[0029] Sera from kidney transplant patients were firstly detected by flow cytometry of random human umbilical vein endothelial cells (HUVEC) for AECAs, and then HLA antibody detection kit and MICA antibody detection kit were used to detect HLA antibody and MICA antibody. The serum meeting the following conditions was used as Specimens for further research: (1) serum creatinine level > 400umol / L, (2) random HUVEC flow cytometry cross-reaction positive, (3) anti-HLA or MICA antibody positive, or HUVEC positive. (4) The sta...

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Abstract

The invention discloses a body endothelial cell transplant antigen and application and a clinical detection kit thereof. The endothelial cell antigen is keratin 1. The kit detecting keratin 1 antibody is used for assessing the risk and state of clinical transplant rejection. According to the body endothelial cell transplant antigen and application and the clinical detection kit thereof, a new study goal of exploring the reason why clinical organ transplant rejection happens and how to predict the occurrence of rejection reaction is provided, and a new thought of building a new clinical organ transplant risk assessment system is provided.

Description

technical field [0001] The invention belongs to the technical field of evaluation and prediction of immune rejection of organ transplantation, and in particular relates to a human endothelial cell neotransplantation antigen related to transplantation rejection and its application. Background technique [0002] Organ transplant rejection remains an important barrier to successful transplantation. Kidney transplantation is by far the most ideal treatment to save the lives of patients with end-stage renal failure. The hyperacute, acute and chronic rejection after organ transplantation makes the graft unable to perform normal physiological functions in the recipient, the organ is rejected, and the function is lost, which seriously affects the quality of life and life safety of the transplant recipient. Graft major histocompatibility antigens include ABO blood group antigens and human leukocyte antigens (HLA). However, acute rejection can also occur in the absence of donor HLA ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47G01N33/68
CPCC07K14/4741G01N33/6848G01N33/6854G01N2800/245G01N2800/50
Inventor 邹义洲郭旭丽胡娟罗伟光
Owner CENT SOUTH UNIV
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