130results about "Cytokeratin" patented technology

A process for the preparation of soluble proteins of high molecular weight with little or no damage to the structural integrity of the proteins. The process is economically and environmentally acceptable by virtue of the cost of reagents that are used, and the recycling of some of those reagents, and is suitable for the production of soluble proteins on a large scale. The process includes a first stage using oxidative sulfitolysis followed by a second stage using mild conditions to extract the soluble protein. In the case of wool as the protein source the process leads to the production of soluble keratin proteins fractionated into the classes S-sulfonated keratin intermediate filament proteins and S-sulfonated keratin high sulfur proteins.

A method of drilling or gas-lifting is disclosed where the methods including the use of a foaming agent and a gas, where the foaming agent is a keratin and the hydrostatic pressure of the fluid in the well is for a portion of the drilling or gas-lift operation less than an hydrostatic pressure of the formation being drilled or under production.

Methods for producing biocompatible heterogeneous proteinaceous networks crosslinked with a heterogeneous crosslinking agent, and the novel crosslinked networks.

The present invention relates to a method of producing keratin hydrolysate comprising the steps of: i) reacting keratin material with a protease; and ii) reacting keratin material with a chemical oxidant; wherein step ii occurs: a) after step i); b) during step i) when the selected protease hydrolyses under the pH conditions used for the chemical reaction and / or c) prior to step i) when the selected protease hydrolyses under the reaction conditions used for the chemical reaction; keratin hydrolysate so produced and uses thereof.

A process for separating keratinous proteins from a keratin-containing material, the process comprising the steps of: subjecting the keratin-containing material to reduction in a liquid medium to solubilise the keratinous proteins under conditions that minimise hydrolysis of the keratinous proteins, to yield a solution of keratinous proteins and undissolved solids; subjecting the solution of keratinous proteins to peroxide oxidation, without any intervening keratin precipitation step; and separating the solution of keratinous proteins from the undissolved solids prior to, at the same time as, or following the oxidation step. Preferred conditions for performing the reduction step involve contacting the keratin-containing material with a solution of an alkali metal sulfide reducing agent at a temperature of between 25 C and 50 C for a time of between 30 and 90 minutes, assuming atmospheric pressure. The peroxide oxidation is suitably carried out within not more than 4 hours after the reduction step, and involves reducing the pH of the solution to a level not less than pH 10, although pH 11.3 is most preferred. The product is demonstrated to have a principal fraction that has a molecular weight above 10 kDa, reflecting that the disulfide bonds in the keratinous proteins are broken without hydrolysis of the proteins.

Biomolecular conjugates are provided which comprise the conjugation product of a repeat sequence protein polymer and at least one active agent. Additional aspects provide methods for their manufacture and various industrial and consumer applications.