Taxol/glucan synchronous-assembled micro/nano-fiber drug loading system and preparation method thereof

A micro-nano fiber and paclitaxel technology, applied in the field of biomedicine, can solve the problems of low drug loading rate, easy sudden release of drugs, poor stability of drug loading system, etc., achieve high drug loading rate, stable and slow drug release, and improve cancer curative effect. Effect

Inactive Publication Date: 2017-04-19
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the drug-loading system based on the self-assembly method generally has poor stability, low drug-loading rate, and easy drug release.

Method used

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  • Taxol/glucan synchronous-assembled micro/nano-fiber drug loading system and preparation method thereof
  • Taxol/glucan synchronous-assembled micro/nano-fiber drug loading system and preparation method thereof
  • Taxol/glucan synchronous-assembled micro/nano-fiber drug loading system and preparation method thereof

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Embodiment 1

[0039] 1. Preparation of paclitaxel / dextran co-assembled micro-nanofibers

[0040] a. First mix glacial acetic acid according to the ratio of 1:99 to prepare 1% (V / V) acetic acid solution, then dissolve a certain amount of dextran in 1% acetic acid solution to obtain a series of gradient solutions (0.5, 1.0, 1.5 , 2.0, 2.5, 3.0 mg / mL).

[0041] b. Paclitaxel was dissolved in absolute ethanol to obtain a 2 mg / mL paclitaxel solution.

[0042] c. After the dextran and paclitaxel are completely dissolved, slowly drop the paclitaxel solution into the dextran solution at a ratio of 1:1 (V / V).

[0043] d. After the paclitaxel / dextran mixed solution was sonicated for 30 minutes, it was left to stand in a refrigerator at 4°C for 3-4 days, and micro-nano fibers were formed when the mixed solution became opaque and milky white.

[0044]e. Centrifuge the opaque milky white liquid to collect the precipitate, then transfer it to a dialysis bag, successively dialyze and purify in a mixed s...

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Abstract

The invention relates to a taxol/glucan synchronous-assembled micro/nano-fiber drug loading system and a preparation method thereof. The micro/nano-fiber drug loading system is composed of taxol and glucan which is 40,000 in average molecular weight. Drug loading micro/nano-fibers are formed as a taxol solution and a glucan solution are mixed at a volume ratio of 1 to and two molecules are cooperated and synchronously assembled in a mixed solution under the action of non-covalant bonds. In comparison with an existing taxol drug loading system, the drug loading system provided by the invention is greater than 70% in drug loading rate, and the drug loading system is stable and slow in drug release and low in toxicity on normal cells while significant in effect of killing cancer cell. The micro/nano-fibers provided by the invention, with the advantages, are safer and more effective in clinical application as the drug loading system. Furthermore, the preparation method provided by the invention has the advantages of being simple, safe and cheap in equipment, low in environmental pollution and the like. Therefore, the taxol/glucan synchronous-assembled micro/nano-fiber drug loading system has a broad application prospect in the aspect of treating cancers.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a drug-carrying system, in particular to a paclitaxel / dextran co-assembled micro-nano fiber drug-carrying system and a preparation method thereof. Background technique [0002] Paclitaxel (PTX), a cancer treatment drug extracted from the trunk and bark of Taxus yew and widely used clinically. Studies have shown that paclitaxel hinders the synthesis of spindle filaments by binding to tubulin, and eventually cancer cells die because they cannot mitosis. Paclitaxel has a significant therapeutic effect on cancer, especially breast cancer and ovarian cancer, which seriously threaten women's health and life. However, the clinical application of paclitaxel is greatly limited due to its hydrophobicity (solubility less than 1 mg / mL). At present, paclitaxel intravenous injection is generally used clinically, and the injection is a mixed solvent of polyoxyethylene castor oil and ethanol ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K31/337A61K47/36A61P35/00
CPCA61K9/0092A61K31/337A61K47/36
Inventor 朱邦尚周彤彤
Owner SHANGHAI JIAO TONG UNIV
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