Test method for research on toxicity of loach in sediment-water

A test method and sediment technology, applied in the field of study of loach toxicity in sediment-water body, can solve the problem of lack of sediment toxicity data

Inactive Publication Date: 2017-04-19
NANKAI UNIV
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  • Abstract
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Problems solved by technology

[0006] At present, the model organisms recommended as sediment toxicity tests in the world mainly include benthic invertebrates such as chironomids and worms. There is no standard for toxicity tests using benthic fish as test organisms. There is also a lack of sediment toxicity data

Method used

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  • Test method for research on toxicity of loach in sediment-water

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Experimental program
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Embodiment 1

[0029] 1. The domestication of loach

[0030] According to the experimental requirements, select loach seedlings with a body length of 3 to 5 cm and a wet weight of 0.7 to 1.0 g. They were domesticated in the laboratory for two weeks. The water was changed once a day, and the fish food was sold twice a week. The dead loach was picked out in time. The survival rate of loach during domestication is more than 90%. The loach was fasted for 24 hours before the test.

[0031] 2. Exposure test

[0032] Test container: length×width×height=20×10×30cm, sediment height is 4cm, the volume ratio of sediment to overlying water is 1:4, the overlying water is about 16cm high, and the drain outlet is 9cm high.

[0033] A laboratory simulation of the toxicity of polycyclic aromatic hydrocarbons in sediment-water to loach. The sediment was spiked with acetone as a cosolvent. The test group has a blank concentration group, a reagent blank group, and treatment groups A, B, and C. , D, E, 4 parallel in e...

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Abstract

A test method for research on toxicity of loach in sediment-water comprises the following specific steps: 1, domestication of loach; 2, simulation of a sediment-water toxicity system; 3, exposure test; and 4, analysis of results. The invention has the following advantages: 1, a common biological test method is adopted; the test device is simple and reasonable and is simple to operate; smooth and effective loach toxicity test can be guaranteed; toxicity of loach under the influence of sediment can be reflected; most of labs can meet requirements of the experiment in the invention; and the method can be promoted and used; and 2, the test method of the invention can provide powerful reference and supplement for other benthic fish sediment-water toxicity test and can provide a reasonable technology for formulation of sediment quality criteria, effective practice of ecological assessment, comprehensive treatment of environmental pollution and protection of ecological balance.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of testing methods for the biological system effects of chemicals, and specifically relates to a testing method for studying the toxicity of loach in a sediment-water body. [0003] Background technique: [0004] After pollutants enter the water body through processes such as direct discharge, surface runoff, and dry and wet sedimentation, they will gradually accumulate in the sediments of the water body and become a reservoir of pollutants, which will cause harm to the organisms living in it. Under certain conditions, once again Released to water bodies, causing secondary pollution. Therefore, water sediments play a vital role in the accumulation of pollutants in aquatic organisms and their migration and transformation in the water environment, and are closely related to the entire ecosystem and human health. Heavy metals and persistent organic pollutants can accumulate to high levels in the sediments of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 祝凌燕沈兆爽韩雨薇钟文珏张彦峰
Owner NANKAI UNIV
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