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SSR core primer set based on Pennisetum purpureum Schum transcriptome sequence development and its application

A transcriptome sequence and core primer technology, applied in the field of SSR primer sets, can solve the problems of unseen transcriptome sequencing technology, no reports on the development and application of molecular markers, etc. Effect

Inactive Publication Date: 2017-04-26
SICHUAN AGRI UNIV
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Problems solved by technology

So far, no transcriptome sequencing technology has been applied to Pennisetum plants, and there is no report on the development and application of EST-SSR molecular markers for a certain species of Pennisetum

Method used

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  • SSR core primer set based on Pennisetum purpureum Schum transcriptome sequence development and its application
  • SSR core primer set based on Pennisetum purpureum Schum transcriptome sequence development and its application
  • SSR core primer set based on Pennisetum purpureum Schum transcriptome sequence development and its application

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with embodiment.

[0028] Primers were designed for the SSR molecular markers of Elephant grass developed based on RNA-seq, and 21,213 pairs of SSR primers were obtained. The present invention has carried out verification and transferability research on 50 pairs of SSR core primer sets. The sequences of 50 pairs of SSR core primer sets are shown in Table 1 below, and their nucleotide sequences are shown in SEQUENCE ID NO.1-100 of the sequence table.

[0029] Table 1 SSR core primer set information developed based on Elephant grass transcriptome sequence

[0030]

[0031]

[0032]

[0033]

[0034] The above-mentioned SSR core primer set developed based on the Elephant Grass transcriptome sequence can be applied in the analysis of Elephant Grass genetic diversity and in the analysis of Elephant Grass germplasm genetic pedigree, and its analysis method includes the following steps:

[0035] ...

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Abstract

The invention discloses a SSR core primer set based on Pennisetum purpureum Schum transcriptome sequence development and its application. The SSR core primer set comprises 50 pairs of primers and the primers have nucleotide sequences shown in SEQUENCE ID NO. 1 to 100 in the sequence table. The invention also relates to a use of the SSR core primer set in Pennisetum purpureum Schum genetic diversity analysis and Pennisetum purpureum Schum germplasm genetic genealogy analysis. The 50 pairs of the SSR primers are derived from Pennisetum purpureum Schum spire transcriptome sequences. Compared with the existing Chinese pennisetum common SSR primers, the SSR core primer set has the advantages of high polymorphism, stable amplification, good repeatability and statistics convenience. The primers are successfully applied to Pennisetum purpureum Schum genetic diversity analysis and Pennisetum purpureum Schum germplasm genetic genealogy analysis, increase the amount of SSR primers used by Pennisetum purpureum Schum, can be effectively used for Pennisetum purpureum Schum genetic diversity analysis, identification of variety, fingerprinting construction and molecular marker-assisted breeding, and other Chinese pennisetum related sturdy. The SSR core primer set is suitable for promotion and application in the field of biotechnology.

Description

technical field [0001] The invention relates to the field of SSR primer sets, in particular to an SSR core primer set developed based on the Elephant Grass transcriptome sequence and its application. Background technique [0002] Elephant grass is a plant of the genus Hemarthria R.Br., which is an annual or perennial herb and is an important pasture in tropical, subtropical and temperate regions. There are about 140 species in the world, most of which are native to Africa. It is distributed in all provinces and regions from Northeast my country and North China through East China, Central South and Southwest China; it mostly grows in fields, wastelands, roadsides and hillsides at an altitude of 50-3200 meters. Plants of the genus Pennisetum grow fast, have high biomass, and are rich in nutrients. They have excellent characteristics such as high temperature resistance, drought tolerance, relatively waterlogging resistance, and high yield. It is mostly high-quality forage; it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 黄琳凯周思凡张新全严海东黄秀王成然武炳超曾兵陈佩琳蒋林峰
Owner SICHUAN AGRI UNIV
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