Efficient proliferation medium for embryogenic callus of Picea tianshanensis
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A technology of embryogenic callus and proliferation medium, which is applied in the directions of plant regeneration, horticulture, horticultural methods, etc., to achieve the effect of shortening the breeding cycle
Inactive Publication Date: 2019-01-04
INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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Some progress has been made in the induction culture of somatic embryo callus of P. to report
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Embodiment 1
[0108] The high-efficiency proliferation medium for embryogenic callus of Picea tianshanensis of the present invention is an improved SH proliferation medium, and the specific formula is as follows:
[0112] Potassium dihydrogen phosphate KH 2 PO 4 340mg / L
[0113] trace elements
[0114]
[0115] iron salt
[0116] Sodium iron ethylenediaminetetraacetate (FeNaEDTA) 65.1mg / L
[0117] organic matter
[0118]
[0119]
[0120] exogenous growth regulator
[0121] 2,4-D 1.10mg / L
[0122] 6-BA 0.44mg / L.
[0123] The preparation method of culture medium of the present invention is:
[0124] During preparation, firstly, after adding the formula amount of each component except L-glutamine per liter, pure water is used as a solvent, after stirring evenly, the pH value is adjusted to 5.8±0.02, and then Gelrite (carrageenan) is added as ...
Embodiment 2
[0128] The high-efficiency proliferation medium for embryogenic callus of Picea tianshanensis of the present invention is an improved BM2 proliferation medium, and the specific formula is as follows:
[0132] Potassium dihydrogen phosphate KH 2 PO 4 340mg / L
[0133] trace elements
[0134]
[0135] iron salt
[0136] Sodium iron ethylenediaminetetraacetate (FeNaEDTA) 65.1mg / L
[0137] organic matter
[0138]
[0139]
[0140] exogenous growth regulator
[0141] 2,4-D 1.10mg / L
[0142]6-BA 0.44mg / L.
[0143] The preparation method of the culture medium of this example and the method of using it to proliferate the embryogenic callus of Picea tianshanensis are the same as those in Example 1.
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Abstract
The invention provides a pinaceae schrenkiana embryonic callus efficient multiplication culture medium which is an improved an SH multiplication culture medium or an improved BM2 multiplication culture medium. A technical platform for pinaceae schrenkiana embryonic callus efficient multiplication is established, culture mediums, namely improved SH, DCR, 1 / 2DCR, BM and MSG culture mediums for pinaceae schrenkiana embryonic callus efficient multiplication, can be confirmed; the optimal pinaceae schrenkiana embryonic callus efficient multiplication culture medium is improved SH; and culture conditions of pinaceae schrenkiana embryonic callus efficient multiplication are provided. A solid theoretic basis for relatively wide application of pinaceae schrenkiana embryonic callus efficient multiplication is achieved, besides, the technical platform for pinaceae schrenkiana embryonic callus efficient multiplication is established, and a study basis for pinaceae schrenkiana genetic improvement germplasm innovation and breeding period shortening is made.
Description
technical field [0001] The invention belongs to the technical field of asexual reproduction, and in particular relates to a high-efficiency proliferation medium for embryogenic callus of Picea tianshanensis. Background technique [0002] Plant somatic embryogenesis (somatic embryogenesis) is a process in which diploid or haploid somatic cells develop into new individuals through a similar pathway to zygotic embryogenesis without sex cell fusion under certain conditions. The occurrence of somatic embryos in plant tissue culture is not only universal, but also has the characteristics of large number, fast speed and complete structure. It is an effective large-scale asexual reproduction method in application. [0003] At present, Tianshan spruce produces offspring mainly through sexual reproduction. Because of the low seed maturity rate, slow growth, and difficulty in breeding and breeding, asexual reproduction has always been valued by forestry decision-making departments in ...
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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 伊丽米努尔雪合拉提·伊明诺夫夏衣达·雪合拉提哈丽努尔·阿不力米提刘永萍
Owner INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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