Method for breeding mulberry variety through colchicine mutagenesis

A technology of colchicine and mulberry, which is applied in the field of plant variety cultivation, can solve the problems of low screening rate, high lethality rate, and low positive mutation rate, so as to increase the speed and probability of cultivation, increase the rate of positive mutation, and reduce mutagenesis The effect on lethality

Inactive Publication Date: 2017-05-24
山东省蚕业研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the above method has defects such as low forward mutation rate and high lethality rate, which leads to the problem of low screening rate

Method used

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  • Method for breeding mulberry variety through colchicine mutagenesis
  • Method for breeding mulberry variety through colchicine mutagenesis
  • Method for breeding mulberry variety through colchicine mutagenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of method utilizing colchicine mutagenesis to select and breed mulberry species, the steps are as follows:

[0034] (1) Cutting seedlings are more than 50cm high, young mulberry, adult mulberry spring cuts or new shoots after five leaves grow after summer cuts, select strong branches, full terminal buds, and no pests and diseases. Fill the terminal bud with the variable agent (inject with a No. 4 needle, the amount is based on filling the terminal bud without overflowing, and the injection volume is 1ml), repeat the treatment once after 3 days, and obtain the mutagenic material;

[0035] The mutagen is an ethanol solution containing a mass concentration of 0.4% colchicine and 2 mg / L 6-benzylpurine (6-BA);

[0036] (2) After culturing the mutagenic material obtained in step (1) for 15 to 20 days, select the mutated bar, identify the material of the chromosomal variation, and then carry out the breeding of the induced branch, after the positive mutation screening o...

Embodiment 2

[0056] Utilize the method for the colchicine mutagenesis breeding mulberry variety as described in embodiment 1, the difference is that,

[0057] The mutagen was 0.4% colchicine and 1.5, 2, 2.5 mg / L 6-benzylpurine ethanol solution respectively. The mulberry variety 792 was selected to induce the adult mulberry. The mutation rate, The experimental results of lethality rate and positive mutation rate are shown in Table 1:

[0058] Table 1

[0059]

Embodiment 3

[0061] Utilize the method for the colchicine mutagenesis breeding mulberry variety as described in embodiment 1, the difference is that,

[0062] The adult mulberry, young mulberry, and cutting seedlings were treated, and the paired sample T test was performed on the mutation rate, lethality rate, and positive mutation rate using spss19 software. The results are shown in Table 2. The results show that there is no significant difference between the three types of treatments, The inducer was used to treat mature mulberry, young mulberry and cutting seedlings, and the effect difference was not significant.

[0063] Table 2

[0064]

[0065] Note: The adult mulberry variety is Xuan 792, and the young mulberry and cutting seedlings are Lucha 1.

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Abstract

The invention relates to a method for breeding a mulberry variety through colchicine mutagenesis. The method comprises the following steps: (1) performing seedling cuttage, selecting new shoots of young mulberries and adult mulberries emerging after spring cutting or summer cutting, and treating terminal buds with a mutagenic agent to obtain a mutagenesis material; and (2) culturing the mutagenesis material for 15-20 days, selecting a variation strip, performing chromosome identification, determining a chromosome variation material, then breeding an induced branch, performing character direct mutation screening and genetic stability screening to obtain a new variety, grafting, and expanding propagation to establish an improved variety scion garden. According to the invention, the terminal buds of mulberries at a special period are treated by using colchicine and 6-benzylpurine as the mutagenic agent first; and in comparison with the existing conventional mutagenesis method, the direct mutation rate is obviously increased, the mutagenic lethality rate is reduced, and the breeding speed and probability of new species can be obviously be improved.

Description

technical field [0001] The invention relates to a method for using colchicine to mutate and breed mulberry varieties, and belongs to the technical field of plant variety cultivation. Background technique [0002] Mulberry (scientific name: Morus alba L.): Moraceae Morus, deciduous tree or shrub, up to 15 meters high. The tree body is rich in whey, and the bark is yellowish brown. The leaves are ovate to broadly ovate, with pointed leaf apex, round or shallow heart-shaped leaf base, coarsely serrated edges, and sometimes irregular splits. The leaves are glabrous and shiny, with sparse hairs on the dorsal veins. Dioecious, flowering in May, catkins. The fruit ripening period is from June to July. The conglomerate fruit is oval or cylindrical, black-purple or white. Light-loving, slightly shade-tolerant when young. It likes warm and humid climate and is hardy to cold. Drought-tolerant, water and humidity resistance is extremely strong. Native to central and northern Chin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H1/04
CPCA01H1/06A01H1/04
Inventor 陈传杰杜建勋董亚茹赵东晓孙景诗王照红朱红王向誉孙日彦
Owner 山东省蚕业研究所
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