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Gathering device based on ion concentration polarization technology and preparation method of gathering device

A concentration polarization and enrichment technology, applied in the field of chemical detection, to achieve the effect of huge application potential, easy automation, simple and fast process

Inactive Publication Date: 2017-05-24
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, detection of low-concentration samples in microfluidic chips remains a challenge

Method used

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  • Gathering device based on ion concentration polarization technology and preparation method of gathering device
  • Gathering device based on ion concentration polarization technology and preparation method of gathering device
  • Gathering device based on ion concentration polarization technology and preparation method of gathering device

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Embodiment Construction

[0047] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings.

[0048] It should be noted that, in the drawings or descriptions of the specification, similar or identical parts all use the same figure numbers. Implementations not shown or described in the accompanying drawings are forms known to those of ordinary skill in the art. Additionally, while illustrations of parameters including particular values ​​may be provided herein, it should be understood that the parameters need not be exactly equal to the corresponding values, but rather may approximate the corresponding values ​​within acceptable error margins or design constraints. The directional terms mentioned in the embodiments, such as "upper", "lower", "front", "rear", "left", "right", etc., are only referring to the directio...

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Abstract

The invention provides a gathering device based on an ion concentration polarization technology. The gathering device comprises a substrate, a micro-channel sealing layer, an ion exchanging membrane and a plurality of electrodes, wherein the substrate comprises a gathering channel, a buffer channel and an ion exchanging membrane fixing groove, the gathering channel comprises an inlet, an inflow section, an outlet and an outflow section, and the buffer channel comprises an inlet, an inflow section, an outlet and an outflow section; the ion exchanging member fixing groove is arranged between the gathering channel and the buffer channel; the micro-channel sealing layer is arranged above the substrate and used for sealing the channels; the micro-channel sealing layer is provided with an ion exchanging membrane permeation groove; the ion exchanging membrane is arranged in the ion exchanging membrane permeation groove and the ion exchanging membrane fixing groove; and the plurality of electrodes are respectively arranged at the inlet and the outlet of the gathering channel and the inlet and the outlet of the buffer channel. The invention also provides a preparation method of the gathering device based on the ion concentration polarization technology. The gathering device based on the ion concentration polarization technology and the preparation method of the gathering device realizes the gathering of different samples with charges, are simple in structure, less in sample consumption, easy in automation implementation, simple and rapid in process, and free from needing an ultra-clean chamber environment.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to an enrichment device based on ion concentration polarization technology and a preparation method thereof. Background technique [0002] Microfluidic chip has the advantages of less sample consumption and easy automation, and has received more and more attention in the process of sample separation, detection and analysis. However, detection of low-concentration samples in microfluidic chips remains a challenge. The enrichment methods mainly include field amplification sample stacking, isotachophoresis, isoelectric focusing, membrane filtration and ion concentration polarization. Among them, the enrichment method based on ion concentration polarization can enrich any charged samples, such as proteins, DNA, fluorescent reagents and ions, etc., and only requires a simple buffer solution, which has great application potential. Contents of the invention [0003] (1) Tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/64G01N1/28
CPCB01L3/5027B01L3/502707B01L3/502753B01L2200/10B01L2300/0861B01L2300/0887G01N1/28G01N21/64
Inventor 佟建华皮海龙边超李洋孙楫舟夏善红
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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