40 results about "Ion concentration polarization" patented technology

The invention provides an ion concentration polarization chip based on a graphene porous membrane and a preparation method of the ion concentration polarization chip and relates to ion concentration polarization chips. The ion concentration polarization chip comprises a glass base, PDMS substrates, a buffer solution water inlet/outlet and a graphene-based porous membrane in a first PDMS substrate, wherein the first PDMS substrate is provided with a Y-shaped groove, and a second PDMS substrate is provided with an I-shaped groove. The preparation method comprises the following steps: preparing graphene-based precursor slurry; producing a chip; and smearing a notch of a Y channel with the graphene-based precursor slurry, and curing, so as to obtain the ion concentration polarization chip. The preparation method has the beneficial effects that the process is simple, and the chip is low in cost and easy to prepare; graphene materials are weakly acidic and have low damage to devices; and the conductivity of the graphene materials is conveniently regulated and controlled, surface functional groups and electric charges are easily regulated and controlled, and the graphene materials can be composited with a large number of materials so as to prepare composite films and devices. Compared with a NaFion-base chip, a relatively stable and relatively large-range exhaustion region can be formed by the graphene-based chip.

The invention provides a parallel seawater desalination device based on ion concentration polarization effect. The parallel seawater desalination device comprises a main channel and a buffer solutionchamber, and the main channel includes a main channel inlet and a main channel outlet. An ion-selective block is disposed in the main channel, and the ion-selective block is prepared by a cation-selective permeable membrane that is only for passing cation through, and the ion-selective block has a plurality of parallel micro-channels inside the main channel along an axis direction. the buffer solution chamber is disposed on the outer wall of the main channel, and the buffer solution chamber is connected to the ion-selective block to allow the ions in the ion-selective block to enter the buffersolution chamber, and the buffer solution chamber is grounded. A power source that is connected to adjustable electrode potentials is provided at the main channel inlet and the main channel outlet, and the potential at the main channel inlet is greater than the potential at the main channel outlet. The device realizes parallelism to the microchannel by punching holes on the ion-selective block, and the number of holes has little influence on the desalination efficiency of the system, which is advantageous for industrial applications.

Exosomes carry microRNA biomarkers, occur in higher abundance in cancerous patients than in healthy ones, and because they are present in most biofluids, including blood and urine, can be obtained non-invasively. Standard laboratory techniques to isolate exosomes are expensive, time-consuming, provide poor purity, and recover on the order of 25% of the available exosomes. We present a new microfluidic technique to simultaneously isolate exosomes and preconcentrate them by electrophoresis using a high transverse local electric field generated by ion-depleting ion-selective membrane. We use pressure-driven flow to deliver an exosome sample to a microfluidic chip such that the transverse electric field forces them out of the cross flow and into an agarose gel which filters out unwanted cellular debris while the ion-selective membrane concentrates the exosomes through an enrichment effect. We efficiently isolated exosomes from 1×PBS buffer, cell culture media and blood serum. Using flow rates from 150 μL/hr to 200 μL/hr and field strengths of 100 V/cm, we consistently captured between 60% to 80% of exosomes from buffer, cell culture media, and blood serum as confirmed by both fluorescence spectroscopy and nanoparticle tracking analysis. Our microfluidic chip maintained this recovery rate for more than twenty minutes with a concentration factor of 15 for ten minutes of isolation.

Ion Concentration Polarization (ICP) purification devices and methods for building massively-parallel implementations of the same, said devices being suitable for separation of salts, heavy metals and biological contaminants from source water.

The invention belongs to the field of salt lake lithium-extraction equipment, and particularly relates to an ion concentration polarization effect based salt lake lithium-extraction device. The ion concentration polarization effect based salt lake lithium-extraction device comprises a main channel, wherein a first buffer solution tube, a brine tube for introducing an extracting stock solution intothe main channel, a lithium-rich solution cavity tube and a second buffer solution tube are sequentially arranged on the main channel; the two ends of the main channel are separately equipped with afirst outlet and a second outlet; and a first negative ion selective block with a plurality of channels and a second negative ion selective block with a plurality of channels are arranged inside the main channel. Locally reinforced electric fields, caused by ion concentration polarization effect, in the channel are utilized to realize lithium iron enrichment and lithium-magnesium separation; and the solution is driven to flow through pressure intensity at the inlet and outlets, and a lithium solution with high concentration is collected by an opening in a pipeline. For different direct parallel layout of micron-level channels, a great number of micron-level channels are processed on an ion selecting material, so that large-scale parallel layout of the micro channels is realized.

The invention provides an ICP-based lateral-flow chromatographic strip for immunodetection. The ICP-based lateral-flow chromatographic strip comprises a Nafion permselective membrane, a label pad, a test line, a control line and water-absorbing pads. In detection, the water-absorbing pads located at the two ends of the strip are wetted; then a to-be-detected sample is added to a sample introduction point on the strip; and a negative electrode is applied to the upper stream of the Nafion permselective membrane of the strip, and a positive electrode is applied to the other end of the strip. Under the action of an electric field, the to-be-detected sample moves towards the positive electrode and gathers due to ICP effect and is eventually captured by the test line. After completion of energization, a dry water-absorbing pad is allowed to contact with the part, located at the lower stream of the control line, of the strip, and the label pad is allowed to contact with the strip at the same time; a buffer solution is used for flushing the label pad; under chromatographic action, a labeled substance is separately bonded with the sample on the test line and with the control line; and thus, immunodetection is accomplished. According to the invention, the to-be-detected sample on the strip is enriched and directionally driven via the ICP effect under the action of the electric field and are captured by the test line, so detection sensitivity is improved.

The invention discloses a double-membrane-chamber membrane capacitance desalination device with continuous water production capacity. According to of the structure of the device, a double-membrane-chamber membrane capacitance assembly is provided with two membrane chambers, graphite electrodes, an activated carbon fiber cloth, a cation exchange membrane, an anion exchange membrane and the like. Araw water tank is connected with a water inlet pump, an outlet of the water inlet pump is connected with the two membrane chambers of the double-membrane-chamber membrane capacitance assembly separately, and treated water enters a water outlet converter to be divided into purified water and concentrated water. The water outlet converter is provided with two water outlets, and the purified water isdischarged from the fresh water outlet; the concentrated water is discharged from the concentrated water outlet and returns to the raw water tank. The water outlet converter is provided with a signalline connected with a time relay, a time signal is transmitted to an electrode transfer device, and the electrode transfer device controls the conversion of positive and negative polarities of the two graphite electrodes. The system combines an ion concentration polarization technology with a membrane capacitance desalination technology, the desalination process of the fresh water chamber and theregeneration process of the concentrated water chamber can be synchronously achieved, and then continuous water outflow can be achieved. Electric energy released in the regeneration process of the concentrated water chamber can be recycled in situ, and the energy-saving purpose is effectively achieved.

Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.

Described are methods for detecting and quantifying biomolecules such as polynucleotides or polypeptides in an electrophoresis matrix using ion concentration polarization and nanoparticle aggregation.

A portable device for removal of metabolic waste from the blood of patient having kidney disease or in need of hemodialysis is provided. Methods of hemodialysis employing the portable device beneficially obtain a dialysate by electrokinetic means from excess fluid in the peripheral blood of the patient in need thereof. The methods employ a branched microfluidic channel for the use of ion concentration polarization to separate charged from neutral species in blood to obtain the dialysate for undergoing hemodialysis. Beneficially the methods and device are resistant to biofouling, remove the need for a dialysate and/or dialysate reservoir, and provide a disposable, wearable device.

The invention relates to a 3D sea water desalination chip device based on an ion concentration polarization principle. The 3D sea water desalination chip device comprises a base adopting 3D printing and a shell adopting 3D printing, a convexly formed buffer solution channel is formed in the base, and a micro-nano membrane channel, a desalted water channel, a concentrated saline water channel and a water outlet are also formed in the base; after the shell and the base are combined, a saline water channel is formed. After a 3D printing material is adopted for combination, fit is tight, no seam is formed, and leakproofness is extremely good; the base and the shell are clamped, connected and fixed by virtue of a 3D printing surrounding edge, the fixed position is accurate, glue or a nail does not need to be used, the integrity can be guaranteed, and the device can not be destroyed by the glue or nail; the manufacturing cost is low, and the device has mass production potency and is recyclable; the device is supported by 3D printing, so that the precision of the dimensions and shape of the device is not limited by familiarity degree of skills of workers, and the obtained finished product has relatively good dimensions and precision; the ion concentration polarization principle is utilized for carrying out sea water desalination, the energy consumption is low, the desalination efficiency is high, and no harm is done to the environment.

High-throughput microfluidic devices comprising one or more fluidic microchannels each with at least one flow-through 3D structure comprising a 3D electrode, or alternatively a 3D permselective structure, and optional secondary bead bed(s) are disclosed. Such devices can be used for counter-flow focusing of charged species via ion concentration polarization and in situ quantification of electrokinetically enriched charged species from an ionically conductive solution by both optical and electrical detection.