Rapid identification method of PVX viruses and application thereof
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An identification method and virus technology, applied in the field of agricultural detection, can solve the problems of differentiation, high cost, high technical requirements and equipment requirements
Inactive Publication Date: 2017-05-24
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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[0004] Potato virus identification includes advanced electron microscope technology, ELISA technology, monoclonal antibody technology, etc., and the use of indicator plants, such as Gomphrenaglobosa L, Daturastramonium, Datura glabra ( Daturametel), Capsicumannum, NicotianatabacumL, Nicotianasamsun, Nicotianaglutinosa, Enopodiumalbum, Chenopodium amaranticolor, etc. It is based on the verification that can be distinguished, and the above simple list makes it difficult to distinguish different virus infections from each other, which will cause misjudgment
[0005] At present, although the preventi...
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Embodiment 1
[0020] Using the PVX virus rapid identification method of the present invention in tobacco planting
[0021] Tobacco is planted in the tobacco field, and several rows are planted at intervals in the tobacco ridge to identify hosts, including ordinary tobacco, heart leaf tobacco, cucumber or zucchini, amaranth, amaranth, and datura.
[0022] During the planting period, if mosaic leaves appear on the tobacco leaves, the tobacco leaves with more serious lesions are picked, then ground, and the ground juice is rubbed with the leaves to identify the host.
[0023] After friction, each of the following situations occurs:
[0024] Ordinary fireworks leaves;
[0025] heart leaf tobacco leaves;
[0026] cucumber or zucchini flowers and leaves;
[0027] Amaranth flowers and leaves or withered spots;
[0028] Amaranth Quinoa Blight;
[0029] Datura mosaic or inoculated leaf blight.
[0030] Then it can be judged that the infected virus is PVX virus.
[0031] It was verified under ...
Embodiment 2
[0033] Pretreatment is with embodiment 1, as behind the friction, following situation occurs:
[0034] Ordinary fireworks leaves;
[0035] heart leaf tobacco leaves;
[0036] cucumber or zucchini flowers and leaves;
[0037] Amaranth flowers and leaves or withered spots;
[0038] Amaranth Quinoa Blight;
[0039] Datura mosaic or inoculated leaf blight.
[0040] Then it can be judged that the infected virus is not PVX virus, and it is PVY virus verified by electron microscopy.
[0041] The present invention starts from the traditional indication of plant lesions, and the creative expression is not only to select different indications of various hosts for different diseases to form a scientific and reasonable combination, but also to distinguish different diseases from each other, that is, the same Identifying the host system has different indication effects for different diseases and prevents duplication. It not only achieves the identification of diseases, but also distin...
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Abstract
The invention provides a rapid identification method of PVX viruses. The PVX viruses are propagated in an identification host system and are judged according to the characteristics of different hosts in the identification host system, wherein identification hosts in the identification host system include nicotiana tabacuml, nicotiana glutinosa, cucumbers or zucchini, gomphrena globosal, chenopodium amaranticolor and mandala, the characteristics of the identification hosts which receive PVX propagation are as follows: the nicotiana tabacuml is mottled, or has mottled leaves, half ring spots or necrotic ring spots, the nicotiana globosal has mottled leaves or mottled inoculateion leaves, the cucumbers or zucchini is not infected, inoculation leaves of the gomphrena globosa have red edges, withered spots or yellow spots, the chenopodium amaranticolor is necrotic or has withered spots, and the mandala has mottled leaves and necrotic spots. The method has the advantages of simplicity, low cost and reliable results and can be popularized and used. The invention further relates to application of the rapid identification method of the PVX viruses to agricultural cultivation.
Description
technical field [0001] The invention relates to the field of agricultural detection, in particular to a PVX virus rapid identification method and application thereof. Background technique [0002] In agricultural planting, insect pests and diseases have always been the hazards of the planting industry, which are related to the quality and yield of agricultural products, and sometimes even cause the failure of agricultural products. Therefore, the prevention and control of insect pests and diseases has always been extremely important in agricultural planting. link, and pesticides are an effective way to solve this problem. However, with the deepening of the concept of environmental protection and the popularization of healthy food, people's demand for pollution-free and pesticide-free agricultural products continues to increase. According to reports of excessive residues, the damage of pesticide residues to human health is very serious. Therefore, it is necessary to strictly ...
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