Rapid identification method of PVX viruses and application thereof

Abstract

The invention provides a rapid identification method of PVX viruses. The PVX viruses are propagated in an identification host system and are judged according to the characteristics of different hosts in the identification host system, wherein identification hosts in the identification host system include nicotiana tabacuml, nicotiana glutinosa, cucumbers or zucchini, gomphrena globosal, chenopodium amaranticolor and mandala, the characteristics of the identification hosts which receive PVX propagation are as follows: the nicotiana tabacuml is mottled, or has mottled leaves, half ring spots or necrotic ring spots, the nicotiana globosal has mottled leaves or mottled inoculateion leaves, the cucumbers or zucchini is not infected, inoculation leaves of the gomphrena globosa have red edges, withered spots or yellow spots, the chenopodium amaranticolor is necrotic or has withered spots, and the mandala has mottled leaves and necrotic spots. The method has the advantages of simplicity, low cost and reliable results and can be popularized and used. The invention further relates to application of the rapid identification method of the PVX viruses to agricultural cultivation.

Description

technical field [0001] The invention relates to the field of agricultural detection, in particular to a PVX virus rapid identification method and application thereof. Background technique [0002] In agricultural planting, insect pests and diseases have always been the hazards of the planting industry, which are related to the quality and yield of agricultural products, and sometimes even cause the failure of agricultural products. Therefore, the prevention and control of insect pests and diseases has always been extremely important in agricultural planting. link, and pesticides are an effective way to solve this problem. However, with the deepening of the concept of environmental protection and the popularization of healthy food, people's demand for pollution-free and pesticide-free agricultural products continues to increase. According to reports of excessive residues, the damage of pesticide residues to human health is very serious. Therefore, it is necessary to strictly ...

AI Technical Summary

Problems solved by technology

[0004] Potato virus identification includes advanced electron microscope technology, ELISA technology, monoclonal antibody technology, etc., and the use of indicator plants, such as Gomphrenaglobosa L, Daturastramonium, Datura glabra ( Daturametel), Capsicumannum, NicotianatabacumL, Nicotianasamsun, Nicotianaglutinosa, Enopodiumalbum, Chenopodium amaranticolor, etc. It is based on the verification that can be distinguished, and the above simple list makes it difficult to distinguish different virus infections from each other, which will cause misjudgment

[0005] At present, although the prevention and treatment of PVX virus is relatively mature, how to find and identify PVX virus in time has become a key to the removal of PVX virus. Conventional PVX virus identification includes serological identification methods, double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and antigen direct coating method, electron microscope method, etc., the above-mentioned identification methods require high technical and equipment requirements, or high cost, etc., which are not convenient for planters to use, and the general experience judgment is relatively rough , often erroneous judgments

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