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A method for rapid propagation of leek tissue culture

A technology of tissue culture and leek, which is applied in the field of rapid propagation of leek tissue culture, can solve the problems of low plant survival rate and low reproduction coefficient of leek tissue culture, and achieve the goal of increasing the reproduction coefficient, improving the survival rate, and high survival rate Effect

Active Publication Date: 2018-09-18
宁波和养生物科技有限公司
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing leek tissue culture has low reproduction coefficient and low plant survival rate, and needs to be further improved

Method used

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Embodiment Construction

[0022] A method for rapid propagation of leek tissue culture, comprising the following steps:

[0023] (1) Pretreatment of leek seeds

[0024] The variety of leek is Musselburgh; select high-quality leek seeds of the same size, round and plump, rinse them with tap water, and soak the seeds in 50mg / L of GA 3 in the solution for 24 hours, and then placed in a light incubator at 25°C for shading cultivation for one day for later use;

[0025] (2) Disinfection and sterilization of leek seeds

[0026] Take out the above seeds, wash them with an ultrasonic cleaner for 2-3 minutes, rinse them with sterile water for 2-3 times, then disinfect them with 75% alcohol for 35-40 seconds, then rinse them with sterile water for 1-2 times, and then rinse them with 0.1 % mercuric chloride for 5-6 minutes, and finally rinsed with sterile water for 3-4 times;

[0027] (3) Obtaining sterile vaccines

[0028] Inoculate the sterilized seeds on MS medium, and cultivate them in the dark at 25°C. A...

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Abstract

The invention discloses an intermediate propagation method for tissue culture of leek. The intermediate propagation method comprises the following steps: pre-treating leak seeds, sterilizing and disinfecting the leek seeds, harvesting sterile seedlings, preparing cotyledon, stems and root section explants, inducing calluses and buds, performing rooting culture, performing acclimatizating and transplanting and the like. As the cotyledon, the stems and the root sections of the sterile seedlings of the leek can all successfully form regenerated plants in an induced differentiation manner, the utilization rates of different parts of the sterile seedlings are greatly increased, the regeneration frequency of the explants is remarkably increased, and a stable basis is made for intermediate propagation of tissue culture of the leek. In addition, by using a method that saw dust is adopted as a matrix in the transplanting process and water is sprayed to keep the saw dust wet in the period, the survival rate of the transplanted regenerated leek plants can be greatly increased, and a propagation technique for intermediate propagation of tissue culture of the leek is powerfully ensured.

Description

technical field [0001] The invention relates to a method for rapid propagation of leek tissue culture. Background technique [0002] The existing leek tissue culture has low reproduction coefficient and low plant survival rate, and needs to be further improved. Contents of the invention [0003] The object of the present invention is to provide a leek tissue culture rapid propagation method that improves the utilization rate of each part of the aseptic seedling, improves the regeneration frequency of explants, and improves the transplanted survival rate of the regenerated plant of the leek. [0004] Technical solution of the present invention is: [0005] A method for rapid propagation of leek tissue culture is characterized in that it comprises the following steps: [0006] (1) Pretreatment of leek seeds [0007] The variety of leek is Musselburgh; select high-quality leek seeds of the same size, round and plump, rinse them with tap water, and soak the seeds in 50mg / L ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 梁美霞张洪霞
Owner 宁波和养生物科技有限公司
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