Hyperammonemia model of affecting apoptosis gene expression in rat brains and construction method of hyperammonemia model

A technology of gene expression and construction method, applied in the field of clinical medicine

Inactive Publication Date: 2017-05-31
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Application Information

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Problems solved by technology

[0003] To sum up, the existing research focuses on the influence of ammonia on brain metabol...

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  • Hyperammonemia model of affecting apoptosis gene expression in rat brains and construction method of hyperammonemia model
  • Hyperammonemia model of affecting apoptosis gene expression in rat brains and construction method of hyperammonemia model
  • Hyperammonemia model of affecting apoptosis gene expression in rat brains and construction method of hyperammonemia model

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[0016] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0017] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0018] Such as figure 1 As shown, the method for constructing a model in which high blood ammonia affects the expression of apoptosis genes in the brain of rats provided by the embodiments of the present invention includes the following steps:

[0019] S101: 40 experimental animals were divided into 2 groups according to the random principle (statistical random number table method): group A (normal control group) and group B (hyperammonia model group), 20 animals in each group. Group...

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Abstract

The invention discloses a hyperammonemia model of affecting apoptosis gene expression in rat brains and a construction method of the hyperammonemia model. The hyperammonemia model of affecting the apoptosis gene expression in the rat brains is as follows: 40 male rats are equally divided into two groups randomly, namely a normal control group and a hyperammonemia model group; the learning and memorizing functions of the rats are evaluated through a water maze test; the rats are narcotized and then plasma is separated to detect blood ammonia; one part of rat brain tissues is fixed through 4% paraformaldehyde and the other part is stored at 80 DEG C below zero; the rat brain apoptosis condition is detected through a Tunel kit; and the expression of the rat brain tissue Bcl-2 and Bax genes is detected through RT-qPCR. Neuronal apoptosis of the rat brain tissues employing the hyperammonemia model is increased. Due to the multifactor action result in the brain tissues, disequilibrium of Bcl-2 and Bax apoptosis gene expression is an important mechanism.

Description

technical field [0001] The invention belongs to the technical field of clinical medicine, and in particular relates to a model in which high blood ammonia affects the expression of apoptosis genes in rat brains and a construction method thereof. Background technique [0002] A large number of studies have shown that apoptosis (programmed death) is involved in the pathological process of nervous system damage caused by various reasons, such as cerebral ischemia and hypoxic damage. The theory of ammonia poisoning plays an important role in the pathogenesis of hepatic encephalopathy. The toxic effect of ammonia on the central nervous system is mainly realized by interfering with the energy metabolism of the brain, and the previous work of the research group has confirmed that it leads to a certain degree of cognitive dysfunction. However, the existing research focuses on the influence of ammonia on brain metabolism and function, and there are few reports on the effect of ammoni...

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Application Information

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IPC IPC(8): A61K33/20C12Q1/68A01K67/027
CPCA01K67/027A01K2207/20A01K2267/0356A61K33/20C12Q1/6883C12Q2600/158
Inventor 潘陈为王志翊陈未来金玲湘周光耀李杰郑毅诸葛璐方佩佩
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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