Heparin binding protein detection kit

A heparin-binding protein and kit technology, applied in biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2017-05-31
安徽同致生物工程股份有限公司
View PDF2 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many gaps in the research on the molecular mechanism and clinical application of HBP in vivo. How to diagnose sepsis early and evaluate the severity of the disease, so as to achieve early and timely intervention and treatment, imp

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Heparin binding protein detection kit
  • Heparin binding protein detection kit
  • Heparin binding protein detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Heparin-binding protein assay kit, including: enzyme-labeled plate, sample diluent, washing buffer, stop solution, enzyme conjugate, substrate, calibrator, quality control;

[0048] The kit adopts the double-antibody sandwich method. The microwells of the enzyme-labeled plate are coated with HBP monoclonal antibody. Monoclonal antibody binding to form a solid-phase antibody-antigen complex;

[0049] Fully wash with washing buffer to remove unbound components, add enzyme conjugates for incubation, and the enzyme conjugates bind to the solid-phase antibody-antigen complex;

[0050] Wash further to remove unbound components, then add the substrate to incubate, the substrate is catalyzed by the enzyme to become a colored product, then add stop solution to terminate the reaction, and detect the optical density value of each well with a microplate reader; the optical density value is related to the plasma Positively correlated with the concentration of HBP.

[0051] in:

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a heparin binding protein detection kit. A double-antibody sandwich method is adopted and is characterized by comprising the following steps: coating micropores of an ELISA plate with HBP monoclonal antibodies, diluting HBP in plasma as antigen substance by using a sample diluting solution, and combining the antigen substance with the HBP monoclonal antibodies coating a solid phase to form a solid phase-antibody-antigen complex in a first incubation period; fully washing uncombined components by using a washing buffer solution, adding an enzyme conjugate for incubation, and combining the enzyme conjugate with the solid phase-antibody-antigen complex; further washing, removing uncombined components, adding a substrate for incubation, catalyzing the substrate via enzyme to prepare a coloured product, adding a stop solution for stopping reacting, and detecting optical density values of various pores via an enzyme labeling instrument, wherein the size of the optical density values is in positive correlation with the concentration of HBP in the plasma. The heparin binding protein detection kit is a novel immune detection kit for quantitatively detecting heparin binding protein in human plasma in vitro and can contribute strength to medical examination and human health.

Description

technical field [0001] The invention relates to the technical field of immunoassay and disease detection, in particular to a heparin-binding protein assay kit. Background technique [0002] Infectious diseases are a class of diseases that seriously threaten human health. Sepsis is one of the infectious diseases with a high mortality rate. Early diagnosis and treatment play an important role in improving the curative effect and prognosis of sepsis. Therefore, the biggest clinical challenge now is how to diagnose sepsis patients as early as possible. In recent years, heparin-binding protein (HBP) has been confirmed as one of the early diagnostic markers of sepsis. [0003] Sepsis refers to systemic inflammatory response syndrome (systemic inflammatory response syndrome, SIRS) caused by infection, clinically confirmed infection or highly suspected infection. Although sepsis is caused by infection, once it occurs, its occurrence and development follow its own pathophysiologica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/68G01N33/535
Inventor 沈秀军
Owner 安徽同致生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap