Heparin binding protein detection kit

Abstract

The invention provides a heparin binding protein detection kit. A double-antibody sandwich method is adopted and is characterized by comprising the following steps: coating micropores of an ELISA plate with HBP monoclonal antibodies, diluting HBP in plasma as antigen substance by using a sample diluting solution, and combining the antigen substance with the HBP monoclonal antibodies coating a solid phase to form a solid phase-antibody-antigen complex in a first incubation period; fully washing uncombined components by using a washing buffer solution, adding an enzyme conjugate for incubation, and combining the enzyme conjugate with the solid phase-antibody-antigen complex; further washing, removing uncombined components, adding a substrate for incubation, catalyzing the substrate via enzyme to prepare a coloured product, adding a stop solution for stopping reacting, and detecting optical density values of various pores via an enzyme labeling instrument, wherein the size of the optical density values is in positive correlation with the concentration of HBP in the plasma. The heparin binding protein detection kit is a novel immune detection kit for quantitatively detecting heparin binding protein in human plasma in vitro and can contribute strength to medical examination and human health.

Description

technical field [0001] The invention relates to the technical field of immunoassay and disease detection, in particular to a heparin-binding protein assay kit. Background technique [0002] Infectious diseases are a class of diseases that seriously threaten human health. Sepsis is one of the infectious diseases with a high mortality rate. Early diagnosis and treatment play an important role in improving the curative effect and prognosis of sepsis. Therefore, the biggest clinical challenge now is how to diagnose sepsis patients as early as possible. In recent years, heparin-binding protein (HBP) has been confirmed as one of the early diagnostic markers of sepsis. [0003] Sepsis refers to systemic inflammatory response syndrome (systemic inflammatory response syndrome, SIRS) caused by infection, clinically confirmed infection or highly suspected infection. Although sepsis is caused by infection, once it occurs, its occurrence and development follow its own pathophysiologica...

AI Technical Summary

Problems solved by technology

However, there are still many gaps in the research on the molecular mechanism and clinical application of HBP in vivo. How to diagnose sepsis early and evaluate the severity of the disease, so as to achieve early and timely intervention and treatment, improve the cure rate of patients, and improve the quality of life for patients. Prognosis, reducing the total mortality rate, thereby saving medical resources and reducing the burden on patients and society are still a major problem in current clinical work

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