Multiple digital PCR quantitative detection method for transgenic rice

Abstract

The invention discloses a multiple digital PCR quantitative detection method for transgenic rice, and belongs to the field of quantitative detection of transgenic rice. The invention firstly discloses multiple digital PCR quantitative detection primer pairs and probes for detecting transgenic rice KF2, KF6, KF8, KMD1, M12, TT51-1 and G6H1. On the basis, the invention further establishes a multiple digital PCR quantitative detection method for transgenic rice KF2, KF6, KF8, KMD1, M12, TT51-1 and G6H1. The method can realize quantitative detection on a sample simultaneously containing KF2, KF6, KF8, KMD1, M12, TT51-1 and G6H1 transgenic rice or containing several kinds of transgenic rice, is high in stability of quantitative detection of transgenic components, and is an effective method for quantitative detection of rice transgenic components.

Description

technical field [0001] The invention relates to a multiple digital PCR quantitative detection method for transgenic rice, belonging to the field of quantitative detection of transgenic rice. Background technique [0002] The latest data show that in 2015, the global area of ​​GM crops planted reached 181 million hectares, an increase of more than 100 times compared to 1.7 million hectares in 1996 (James, Clive."20th Anniversary (1996 to 2015) of the global commercialization of biotech crops and biotech crop highlights in2015."ISAAA Brief 51(2015).). Modern biotechnology with genetic engineering as the core has widely penetrated into various fields of agriculture, gradually changing the traditional agricultural research and production models. Genetically modified crops have been rapidly and consistently used for commercial production. Whether in developed or developing countries, genetically modified crops have had a huge impact on the environment, economy, energy, health a...

AI Technical Summary

Problems solved by technology

However, most of these studies are currently based on 2-fold digital PCR detection, and only one transformation event of a genetically modified crop can be quantitatively analyzed at a time. The current quantitative labeling requirement is for the quantification of a single component rather than a single transformation event. For example, the European Union’s quantitative labeling The definition is: Food contains materials derived from genetically modified organisms in a proportion not higher than 0.9% of the food ingredients as a single ingredient

Therefore, current digital PCR detection methods are not suitable for the simultaneous quantitative detection of multiple transformation events of a single component in a sample

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