A kind of chondrocyte preparation derived from mesenchymal stem cells and preparation method thereof

A chondrocyte and stem cell technology, applied in skin care preparations, animal cells, vertebrate cells, etc., can solve problems such as low chondrocyte survival rate and proliferation rate, increased pain and scarring of the recipient, and poor transplantation effect. , to achieve the effect of shortening the operation cycle, improving the probability of success, and reducing the discharge rate

Active Publication Date: 2020-01-17
华夏(青岛)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of high price and high excretion rate of the existing materials used in facial plastic surgery, the absorption of the used cartilage tissue and the increase of pain and scars for the patient after taking the cartilage, the low survival rate and value-added rate of the applied chondrocytes, and the transplantation effect In order to overcome the poor problems, the present invention provides a chondrocyte preparation derived from mesenchymal stem cells and a preparation method thereof

Method used

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  • A kind of chondrocyte preparation derived from mesenchymal stem cells and preparation method thereof
  • A kind of chondrocyte preparation derived from mesenchymal stem cells and preparation method thereof
  • A kind of chondrocyte preparation derived from mesenchymal stem cells and preparation method thereof

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Embodiment 1

[0033] Embodiment 1 of the present invention provides a chondrocyte preparation derived from mesenchymal stem cells, including a first injection. The first injection is mainly composed of raw materials, auxiliary material I, and chondrocytes configured with water for injection. Contain described raw material 0.25mg, described adjuvant 10.5mg, described chondrocyte 1 * 10 in water 5 , the raw material is mainly made of the following ingredients in grams by weight:

[0034] Zushi Hemp Extract 0.075mg

[0035] Danshen Extract 0.0875mg

[0036] Dog Ridge Extract 0.0875mg.

Embodiment 2

[0038] Embodiment 2 of the present invention provides a chondrocyte preparation derived from mesenchymal stem cells, including a first injection. The first injection is mainly composed of raw materials, auxiliary material I, and chondrocytes configured with water for injection. Water contains 1.25 mg of the raw material, 1.5 mg of the auxiliary material I, and 1 × 10 chondrocytes 8 , the raw material is mainly made of the following ingredients in grams by weight:

[0039] Zushi Hemp Extract 0.125mg

[0040] Danshen Extract 0.625mg

[0041] Dog Ridge Extract 0.500mg.

[0042] A preparation method of a chondrocyte preparation derived from mesenchymal stem cells, the preparation method comprising the following steps:

[0043] S1. Chondrocytes induced and differentiated using adipose-derived mesenchymal stem cells;

[0044] S2. Preparation of raw materials: Weigh 0.125 mg of Zushima extract, 0.625 mg of Salvia miltiorrhiza extract, and 0.500 mg of dog spine extract according t...

Embodiment 3

[0052] Embodiment 3 of the present invention provides a chondrocyte preparation derived from mesenchymal stem cells, including a first injection. The first injection is mainly composed of raw materials, auxiliary material I, and chondrocytes configured with water for injection. The water contains 0.85 mg of the raw material, 1.0 mg of the auxiliary material I, and 1 × 10 chondrocytes. 7 , the raw material is mainly made of the following ingredients in grams by weight:

[0053]

[0054] The preparation method of this preparation is identical with embodiment 2.

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Abstract

The invention provides a mesenchymal stem cell derived cartilage cell preparation and a preparation method thereof; the mesenchymal stem cell derived cartilage cell preparation comprises a first injection, the first injection is mainly made from a raw material, auxiliary material I and cartilage cells along with injection water, the raw material is mainly made from, by weight, 10-60 parts of Daphne giraldii extract, 10-50 parts of Salvia miltiorrhiza extract, and 10-40 parts of cibot rhizome extract. The first injection provided herein is directly applicable to filling materials for facial cosmetic surgery; by injecting the cartilage cells, it is possible to reconstruct cartilage tissues and effectively decreases the rejection rate; the suitable growth environment lays the basis for the differentiation and proliferation of cartilage cells, graft survival rate of cartilage cells is increased, a patient feels less pain during rhinoplasty or craniofacial repair, and the surgical period is shortened; the mesenchymal stem cell derived cartilage cell preparation is widely applicable to the surgeries, such as craniofacial injury, cartilage scaffold reconstruction, form defect repair, laryngoplasty in otorhinolaryngology, and rhinoplasty.

Description

technical field [0001] The invention relates to the technical field of pharmaceutical preparations, in particular to a mesenchymal stem cell-derived chondrocyte preparation and a preparation method thereof. Background technique [0002] With the development of tissue engineering materials, more and more tissue engineering materials have replaced natural biomaterials for in vivo transplantation. Similarly, the acquisition of chondrocyte tissue engineering materials has opened up a new path for cartilage tissue repair. At present, chondrocytes are widely used as transplant materials in the field of facial plastic surgery, such as correction of craniofacial defects and rhinoplasty. [0003] Plastic and cosmetic surgery is an important part of medical science. With the improvement of material life, people have higher and higher requirements for beauty. Whether they are Chinese or foreigners, there are many people whose nose shape does not meet the aesthetic standards. Therefor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61L27/38A61L27/36A61L27/54A61L27/50A61K8/9789A61K8/73A61K8/64A61K8/55A61K8/44A61Q19/00A61Q19/08
CPCA61K8/44A61K8/55A61K8/64A61K8/73A61K8/735A61K8/97A61K2800/91A61L27/3637A61L27/3817A61L27/3852A61L27/3895A61L27/50A61L27/54A61L2300/30A61L2300/412A61L2400/06A61L2430/06A61Q19/00A61Q19/08
Inventor 汤苏阳
Owner 华夏(青岛)生物科技有限公司
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