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Corncob block edible fungus immobilized strain production and cultivation method

A cultivation method and corncob technology are applied in the field of production and cultivation of the immobilized strains of edible fungi in corncob blocks, and can solve the problems of difficult to accurately control the inoculation amount, inconvenient inoculation of wood chips, and low inoculation efficiency, and achieve large application and The effect of promoting value, shortening the germ cycle, and convenient inoculation operation

Inactive Publication Date: 2017-07-25
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sawdust species use sawdust as the main raw material, add a small amount of bran, and are produced by solid-state fermentation, but the production cycle is too long, usually more than 30 days, and the source of sawdust is limited. In addition, the use of sawdust species in the cultivation process also has the disadvantage of inoculation. Convenience, low inoculation efficiency, difficult to accurately control the inoculation amount and other obvious shortcomings, so it is necessary to develop a new low-cost edible fungus strain with short production cycle, easy inoculation operation, and non-wood chips as culture material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Preparation and cultivation application of immobilized strains of Pleurotus ostreatus and corncobs

[0012] 1. Cut the dry and deseeded corncobs vertically and horizontally in the axial direction, and control its thickness to 1 cm; each short columnar slice is further symmetrically cut into 4 uniform blocks;

[0013] 2. Place every 20 corn cobs in a 250 mL shaker flask, and divide into 100 mL of fermentation medium. The formula (per liter) is 35 g of glucose, 5 g of peptone, 4 g of yeast powder, 1.0 g of potassium dihydrogen phosphate, and sulfuric acid Magnesium 0.5 g; sealed with rubber stopper, sterilized at 121°C for 30 minutes;

[0014] 3. After the culture medium is cooled, insert 10 mL of homogenized liquid strains under aseptic conditions. The preparation method is as follows: transfer the slanted oyster mushroom strain to a fresh slant medium, and activate it by incubating at 25°C for 7 days; the activated strain is smashed with an inoculation rake, and inocul...

Embodiment 2

[0023] Production and cultivation application of Pleurotus eryngii immobilized strains with corn cob as carrier

[0024] 1. Cut the dry and deseeded corncobs vertically and horizontally in the axial direction, and control its thickness to 1 cm; each short columnar slice is further symmetrically cut into 4 uniform blocks;

[0025] 2. Place every 20 corncobs in a 250 mL shaker flask, and add 100 mL of fermentation medium. The formula (per liter) is 35 g of glucose, 5 g of peptone, 4 g of corn flour, 1.0 g of potassium dihydrogen phosphate, and sulfuric acid Magnesium 0.5 g;

[0026] Seal with rubber stopper and sterilize at 121°C for 30 min;

[0027] 3. After the culture medium is cooled, insert 5 mL of liquid strains that have been homogenized (grinding, homogenizer or ultrasonic crushing) under aseptic conditions. The preparation method is as follows: transfer the strain of Pleurotus eryngii on the inclined plane to a fresh inclined plane, incubate at 25°C for 7 days for act...

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Abstract

The invention provides a corncob block edible fungus immobilized strain production and cultivation method. De-seeded corncobs are cut into slices, and each short-column-shaped slice is further cut into four corncob blocks symmetrically; the twenty corncob blocks are placed in a 250 mL shake flask, and then transferred into a 100 mL of liquid fermentation culture medium; 10 mL of liquid strains are inoculated into the corncob blocks for cultivation after cooling, in the cultivation process, edible fungus mycelia are naturally absorbed to and grown on the surfaces of the corncob blocks, so that immobilization of edible fungus mycelium cells is achieved, and cultivation is conducted for a certain time to obtain immobilized strains of the edible fungi. The immobilized strains are used as edible fungus cultivation strains or stored and then utilized. By means of the corncob block edible fungus immobilized strain production and cultivation method, the strain production period can be obviously shortened, the inoculation efficiency and the use efficiency are improved, precise inoculation is achieved, the cultivation production period is shortened, the production cost is reduced, the corncob utilization rate is improved, and the method has great application and popularization values.

Description

technical field [0001] The invention belongs to the field of edible fungi and biotechnology, in particular to a method for producing and cultivating immobilized strains of corncob edible fungi. Background technique [0002] Edible fungi are a type of large fungi that can produce fruiting bodies with extremely high nutritional and medicinal value, and have important economic value. Studies have shown that edible fungi are rich in nutrients, such as protein, unsaturated fatty acids, amino acids, dietary fiber, vitamins, trace elements, etc., and their protein content is significantly higher than that of vegetables. Edible fungi also contain some special flavor substances, which make them delicious. Therefore, edible fungi are a good food resource. In addition, edible fungi also contain a variety of biologically active components, the most famous of which is polysaccharides, which have various pharmacological activities such as anti-tumor, anti-oxidation, anti-aging, and immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14C12N11/02C12R1/645
CPCC12N1/14A01G18/00C12N11/02
Inventor 刘盛荣张维瑞匡云波阮俊峰吴发旺
Owner NINGDE NORMAL UNIV
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