Corncob block edible fungus immobilized strain production and cultivation method
A cultivation method and corncob technology are applied in the field of production and cultivation of the immobilized strains of edible fungi in corncob blocks, and can solve the problems of difficult to accurately control the inoculation amount, inconvenient inoculation of wood chips, and low inoculation efficiency, and achieve large application and The effect of promoting value, shortening the germ cycle, and convenient inoculation operation
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Embodiment 1
[0011] Preparation and cultivation application of immobilized strains of Pleurotus ostreatus and corncobs
[0012] 1. Cut the dry and deseeded corncobs vertically and horizontally in the axial direction, and control its thickness to 1 cm; each short columnar slice is further symmetrically cut into 4 uniform blocks;
[0013] 2. Place every 20 corn cobs in a 250 mL shaker flask, and divide into 100 mL of fermentation medium. The formula (per liter) is 35 g of glucose, 5 g of peptone, 4 g of yeast powder, 1.0 g of potassium dihydrogen phosphate, and sulfuric acid Magnesium 0.5 g; sealed with rubber stopper, sterilized at 121°C for 30 minutes;
[0014] 3. After the culture medium is cooled, insert 10 mL of homogenized liquid strains under aseptic conditions. The preparation method is as follows: transfer the slanted oyster mushroom strain to a fresh slant medium, and activate it by incubating at 25°C for 7 days; the activated strain is smashed with an inoculation rake, and inocul...
Embodiment 2
[0023] Production and cultivation application of Pleurotus eryngii immobilized strains with corn cob as carrier
[0024] 1. Cut the dry and deseeded corncobs vertically and horizontally in the axial direction, and control its thickness to 1 cm; each short columnar slice is further symmetrically cut into 4 uniform blocks;
[0025] 2. Place every 20 corncobs in a 250 mL shaker flask, and add 100 mL of fermentation medium. The formula (per liter) is 35 g of glucose, 5 g of peptone, 4 g of corn flour, 1.0 g of potassium dihydrogen phosphate, and sulfuric acid Magnesium 0.5 g;
[0026] Seal with rubber stopper and sterilize at 121°C for 30 min;
[0027] 3. After the culture medium is cooled, insert 5 mL of liquid strains that have been homogenized (grinding, homogenizer or ultrasonic crushing) under aseptic conditions. The preparation method is as follows: transfer the strain of Pleurotus eryngii on the inclined plane to a fresh inclined plane, incubate at 25°C for 7 days for act...
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