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Method for rapidly propagating in vitro tissue of toona sinensis

A technology of Chinese toon and tissue, which is applied in the field of rapid propagation of Chinese toon isolated tissue, can solve the problems of time-consuming and laborious, long cycle, low breeding coefficient, etc., and achieve the effect of easy repetition, high differentiation rate and wide selection of materials

Inactive Publication Date: 2017-08-04
代燚
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Problems solved by technology

[0009] The first object of the present invention is to provide a kind of method for the quick propagation of Chinese toon isolated tissue, the inventive method can effectively solve the technical problems such as long period, low breeding coefficient, time-consuming and labor-intensive in the traditional breeding process of Chinese toon, for the rapid propagation of Chinese toon, The cultivation of improved varieties provides theoretical guarantee

Method used

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  • Method for rapidly propagating in vitro tissue of toona sinensis
  • Method for rapidly propagating in vitro tissue of toona sinensis
  • Method for rapidly propagating in vitro tissue of toona sinensis

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Embodiment 1

[0063] 1. Culture medium preparation

[0064] 1) Seed germination medium: 1 / 2MS+0.5mg / L GA 3 +0.05mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;

[0065] 2) Callus induction medium: MS+0.15mg / L TDZ+0.3mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;

[0066] 3) Callus differentiation medium: MS+0.1mg / L 6-BA+0.5mg / L KT+0.1mg / L NAA+0.3% sucrose+7g / L agar powder, pH 6.0;

[0067] 4) Rooting medium: 1 / 2MS+0.5mg / L NAA+0.2% sucrose+6g / L agar powder, pH 6.0.

[0068] 2. Explant selection

[0069] 1) Aseptic seedling explants

[0070] Shell the Chinese toon seeds, remove the sundries, and wash them twice with water. The seeds were then soaked in a beaker and placed in a 25°C incubator (12h). Remove the floating seeds, wash the seeds in the lower layer, put them on a plate, cover them with wet gauze, and place them in a 25°C incubator (12h). Take a few seeds, put them in a petri dish, sterilize with 75% alcohol solution for 20 seconds, then sterilize with 0.1% mercuric chloride ...

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Abstract

The invention provides a method for rapidly propagating in vitro tissue of toona sinensis. According to the method, by taking germfree toona sinensis seedlings, matured leaves and tender stems as raw materials, preparing different culture medium combinations, optimally selecting culture mediums suitable for toona sinensis callus induction, little bud differentiation and plant rooting and optimizing a transplanting medium for regenerated seedlings and condition control, Liangdu red toona sinensis can be rapidly cultured; a theoretical premise is provided for building of a toona sinensis seed nursery, development and use of toona sinensis tree seeds and final pushing of regional industrialization development.

Description

technical field [0001] The invention relates to the field of forestry biotechnology, in particular to a method for rapid propagation of toona sinensis isolated tissues. Background technique [0002] Chinese toon [Toona sinensis (A.Juss) Roem] is a perennial deciduous tree of Meliaceae. It likes sunshine and is suitable for growing in fertile sandy soil with large temperature difference between day and night. Toona sinensis originates in the central part of my country, and has been cultivated on a large scale in many parts of the country at present, mainly distributed between the Yellow River Basin and the Yangtze River Basin, especially in Hebei and Shandong. Toona sinensis grows rapidly, the trunk is straight and the material pattern is beautiful. It can be used as a fast-growing material and a high-quality wood-grown vegetable. It has high nutritional value, medicinal value and therapeutic value; in addition, the buds and seeds of Chinese toon It can also be used as indus...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 代燚秦利军曹娟涂家宽陈大荣
Owner 代燚
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