Application of herba rabdosiae to treatment of skin diseases
A skin disease, the technology of Rubescens, applied in the field of pharmaceutical compositions for the treatment of skin diseases related to excessive keratinocyte proliferation, the treatment of skin diseases by Rubescens, and psoriasis
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Embodiment 1
[0058] Embodiment 1: the preparation of Radix Grass Extract
[0059] Take 100g of the aerial part of Dongling grass (from Kangmei Pharmaceutical Co., Ltd., batch number: 141009111), dry, pulverize, then add 5 times the mass of 90% ethanol, and soak at 60°C for 3 hours under ultrasonic treatment. Then filter and take the filtrate. Repeat the above soaking and filtering steps 2 times. The filtrates were combined, and ethanol was recovered from the filtrates, and then the residue was concentrated to obtain about 2 g of Oridinus extract in the form of an extract for subsequent studies.
Embodiment 2
[0060] Example 2: Rubescens significantly inhibits the proliferation of keratinocytes
[0061] All cell culture reagents (DMEM, FBS, etc.) were purchased from Gibico; MTT (thiazolium blue) and DMSO were purchased from Sigma; keratinocytes HaCaT cells were purchased from the American Type Culture Collection (ATCC) cell bank (ATCC number: CRL-2310).
[0062] HaCaT cells were seeded on a 96-well plate at 10,000 cells per well, and each well contained DMEM (Dulbecco's modified eagle medium) supplemented with 10% fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin. liquid, in 5% CO 2 and incubated overnight at 37°C. The next day, the DMSO solution of the ethanol extract of Rubescens japonicus was added to different final concentrations (50, 20, 10, 0 μg / mL), and cultured for another 72 hours. Then, 20 μl of 5 mg / mL MTT solution was added to each well, and the incubation was continued for 4 hours. Aspirate the culture solution in the wells to terminate the ...
Embodiment 3
[0064] Example 3: Rubescens significantly inhibits the expression of inflammatory factors in mouse macrophages
[0065] All cell culture reagents (DMEM, FBS, etc.) were from Gibico; reverse transcription kits and real-time fluorescent quantitative PCR (RT-PCR) kits were purchased from Takara; LPS were purchased from Sigma; mouse macrophage RAW 264.7 In the ATCC cell bank (ATCC number: TIB-71 TM ). RAW 264.7 at 5% CO 2 and cultured at 37°C in DMEM (Dulbecco's modified eagle medium) supplemented with 10% fetal bovine serum (FBS), 100 U / mL penicillin, and 100 mg / mL streptomycin.
[0066] RAW 264.7 cells were cultured in a 6-well plate to a density of 60%, and LPS was added to a final concentration (1 μg / mL). For the experimental group, the DMSO solution of the ethanol extract of Rubescens japonicus was added to different final concentrations (50, 20, 10 μg / mL); for the control group, DMSO was added to the final concentration of 1 μg / mL. A blank group was also set up, to which...
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