A comprehensive two-dimensional high performance liquid chromatograph with a multifunctional switching valve and a chromatographic separation method

Abstract

The invention provides a comprehensive two-dimensional high performance liquid chromatograph provided with a multifunctional changeover valve. The comprehensive two-dimensional high performance liquid chromatograph provided with the multifunctional changeover valve comprises a one-dimensional chromatography mobile-phase liquid storage device M1, a one-dimensional chromatography high-pressure pump P1, a one-dimensional chromatography sampling valve V1, a one-dimensional chromatographic column C1, a one-dimensional chromatography detector D1, a two-dimensional chromatography mobile-phase liquid storage device M2, a two-dimensional chromatography high-pressure pump P2, the multifunctional changeover valve V, multiple two-dimensional separation columns C2-1 to C2-8, a two-dimensional chromatography detector D and a fraction collector F. The comprehensive two-dimensional high performance liquid chromatograph has the outstanding effects as follows: 1, two-dimensional separation is performed by use of multiple two-dimensional chromatographic columns, a fixed phase has multiple selectable varieties, the separation efficiency is high, the separation effect is good, and full-ingredient separation and analysis of complicated samples can be realized; 2, the system is provided with multiple detectors, accurate control of the separation process can be guaranteed, and the separation effect can be improved.

Description

technical field [0001] The invention relates to a comprehensive two-dimensional high-performance liquid chromatograph and a method for comprehensive two-dimensional chromatographic separation, belongs to the technical field of instrument and equipment manufacturing, and is a high-performance liquid chromatography separation and analysis device. Background technique [0002] High-performance liquid chromatography uses high-pressure delivery of mobile phase, high-efficiency stationary phase and high-sensitivity detector for detection. It has the characteristics of high efficiency, rapidity, and wide application. The key technology is widely used in the fields of pharmacy, life science, medicine, food science, petrochemical industry and environmental protection. [0003] The usual high-performance liquid chromatography is composed of a liquid reservoir, a high-pressure pump, a sampler, a chromatographic column, a detector, a recorder, etc. During the application process, a chro...

AI Technical Summary

Problems solved by technology

In the separation process, the two-dimensional separation column is required to be short and the analysis speed is fast, which limits the entire separation effect

For large-capacity preparation and separation, the existing technology cannot meet the demand, and cannot be directly applied to the separation and preparation of components

The existing technology uses a one-dimensional chromatographic column and a two-dimensional chromatographic column for separation. During the separation process, only two stationary phase chromatographic columns can be used for separation at a time, which is difficult to meet the separation needs of complex samples

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