The invention provides a column chromatography device with a warning function. The device solves the problem that the existing chromatography device cannot alarm when the liquid is too much or too little. The device comprises a column body, an upper end socket, a lower end socket and a liquid level alarm, wherein a filler is filled in the column body, the column body is provided with a filler inlet and a filler outlet, the upper end socket is arranged on the upper end of the column body and provided with a liquid inlet, an exhaust port and a back-flushing liquid outlet, the lower end socket is arranged at the lower end of the column body and provided with the liquid outlet, the liquid level alarm is arranged on the upper end socket, and the column body is fixedly connected with the upper end socket and the lower end socket. By arranging the liquid level alarm, the column chromatography device with the warning function provided by the invention is capable of providing the warning function when the liquid is too much or too little under the unwatched state, and the device is reasonable in structure, convenient to operate, low in equipment cost, and suitable for commercial popularization and application.