134 results about "Chromatography detector" patented technology

The present invention is directed to a continuous affinity chromatography method and to an apparatus to be used in such method. The method allows the use of high operational velocity while maintaining high binding capacities.

A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.

A cartridge for chromatography separations comprises a housing; at least a first and a second chromatography column at least partially passing through the housing, each chromatography column comprising a first and a second end fitting operable to connect the chromatography to external tubing of a chromatography system. Connection fittings protruding outside of the housing at each end of each column enable fluidic connection to a chromatograph plumbing system and / or mass spectrometer detector. The cartridge may comprise heaters and temperature sensors and optional sensors to monitor fluid flow rate or pH. A passive identification feature, e.g., a barcode or RFID module may identify the cartridge and its associated chromatography methods to external software. Further, an on-board memory module and controller chip may be used to actively record computer-readable module information, including module history information, the information transferable through a standard interface, such as a universal serial bus (USB) port.

The present invention relates to a simulated moving bed process, wherein at least one adsorbent is washed after binding of target compound and wherein the outlet of wash liquid from the adsorbent is subsequently passed onto another adsorbent for binding of target compound removed by the washing. In one embodiment, the method comprises binding of at least one target compound using three or more adsorbents connected in series and elution of target compound from said three adsorbents. After the binding to an adsorbent, wash liquid is passed across the adsorbent to recover desorbed and / or unbound target compound, and the outlet of such wash liquid is directed to the adsorbent after the next in the series, to which no feed has yet been added. The target compound is recovered by eluting target compound from the washed adsorbents.

The present invention relates to a chromatography column 1 in which the net 129 and / or bed support 131 is integrally joined to a net retaining means, preferably a sleeve 135 that protrudes through the column end plate 5, and / or to a net retaining circumferential ring. Different net retaining means are also presented.

The invention discloses decompressed purge-and-trap processing equipment for a non-volatile organic compound in a water sample and a processing method thereof. The invention is characterized in that the equipment is formed by sequentially connecting a sample heater (1), a purge bottle (2), a trapper (3), a pressure controller (4) and a pump (5), wherein purge gas (6) is connected with the purge bottle (2) through a purge gas inlet (7); the purge bottle is connected with the trapper (3) through a purge gas outlet (8); the trapper (3) is connected with the pump (5) through the pressure controller (4); the gas trapped by the trapper (3) is taken out and then placed into a heater (13) of the trapper; chromatographic carrier gas (14) is connected with a chromatographic injection port (12) in a column oven (11) through the trapper (3) and a transmission pipeline (15); and the chromatographic injection port is connected with a chromatographic detector (9) through a chromatographic column (10). The processing method comprises the following steps: forming a negative pressure environment around a test sample under the action of the pump, and increasing the vapor pressure of the non-volatile organic compound under the negative pressure environment; purging the bottom of the sample by utilizing inert gas so that the target compound can be trapped by the trapper after being blown out from the sample; and finally, resolving the target compound at high temperature and then carrying out chromatographic analysis.

The invention relates to a preparation process of intravenous injection human immunoglobulin, belonging to the field of pharmaceuticals. On a basis of a traditional preparation process of intravenous injection human immunoglobulin with the low protein concentration of 5 percent, a filter pressing method is adopted instead of a centrifuging method in an extraction process. During hyperfiltration, the protein concentration is adjusted to 3-6 percent, a pH value is adjusted to 6.4-6.6 with 0.5 mol / L of NaOH; then, 1 mol / L phosphoric acid-NaOH buffer solution is added to adjust the electrical conductivity which is measured to be 0.175-0.205 s / m at a temperature T of 19 DEG C; and a chromatography method is adopted to carrying out column chromatography and purification by using upper ion exchange columns after the electrical conductivity is adjusted. The protein impurities can be effectively removed, the protein purify and the product yield are improved; maltose or glycin is used as a protector, which benefits the improvement of the stability of the intravenous injection human immunoglobulin; and the glycin is used as the protector, which satisfies the clinical use of diabetics. According to the invention, the intravenous injection human immunoglobulin with the protein concentration of 5-11 percent can be obtained.

An ion chromatogram method uses to test the lithium salt (LiPF6) concentration in lithium ion battery electrolyte. (1) Prepare F- and PO43- standard solution in different concentration and test in ion chromatogram method to protract C-A standard curve. (2) Add reverse chloroazotic acid into the need test electrolyte in equal volume and stay for 12-24 hours, then dilute the solution with secondary deionwater step by step and control its concentration inside the concentration range of standard curve. Test the concentration to F- and PO43- in ion chromatogram method at the same condition with step (1). (3) Calculate the lithium salt concentration C1 of the sample in formula (a) according the concentration to F-, calculate the lithium salt concentration C2 of the sample in formula (b) according the concentration to PO43-. It can test the lithium salt concentration according the concentration to F- and PO43- at the same time, and the data of the two groups complement and consult with each other with high veracity.

A chromatography device and method of use to separate components of a sample are described. The device includes a stationary phase supported by a frame or contained within a chamber in a housing. The stationary phase includes a nano alumina medium that has support fibers having nano alumina fibers attached thereto. Optionally, sorbents are electrostatically adhered to the nano alumina fibers. Chromatographic separations are effected by the mobile phase at pressures of less than 10 bar and at flow velocities up to at least 5 cm / min. An electrical potential can be applied across the medium to foster separation of components.

The present invention is directed to a continuous affinity chromatography method and to an apparatus to be used in such method. The method allows the use of high operational velocity while maintaining high binding capacities.

A chromatographic device for determining sulfide in gas materials and its determination method, comprising a first sampler and a second sampler connected in parallel between the first switching valve and the second switching valve to form a sampling system; the second switching valve A first chromatographic channel and a second chromatographic channel are connected in parallel between the valve and the detector to communicate with the detector respectively to form a chromatographic system. The determination method connects the first sample injector with the first switching valve, connects the first sample injector with the second chromatographic channel through the second switching valve, and measures the content of H2S and COS; The device is connected with the first chromatographic channel to measure the content of each component of the organic sulfide. The chromatographic device of the present invention is simple, quick and accurate to measure the content of high-concentration H2S, COS and trace organic sulfide components in gas materials, and can be used for scientific research experiments with small gas production, and can also be used for various coal pyrolysis gases and coalbed methane in industry. and gas determination of natural gas.

A chromatography column and method for use in a liquid chromatography process includes a telescoping chromatography and piston tubes, with the piston tube having a porous layer partially obstructing flow therethrough. A chromatography clamp is selectively movable between open and closed positions, and configured to extend substantially 360 degrees about the periphery of the chromatography tube when closed. The chromatography clamp is configured to releasably maintain the chromatography tube and piston tube in fluid communication with one another. In particular embodiments, the chromatography clamp includes a ledge extending at an oblique angle relative to the transverse direction, which is configured to cam the clamp towards the closed position upon application of greater than atmospheric pressure within the chromatography column.

The invention relates to a detection method for trace forbidden or restricted iodate and bromate in a cosmetic, and aims to provide the method which has the characteristics of high specificity, high anti-interference capacity and high accuracy. According to the technical scheme, the ion chromatography method for detecting iodate and bromate in the cosmetic comprises the following steps of: (1) preparing a sample: weighing a certain amount of cosmetic and placing in a centrifuge tube, and adding ultra-pure water to keep constant volume for later use; or adding the ultra-pure water and then ultrasonically oscillating, then loading on a centrifuge for centrifugal separation, taking supernatant and keeping constant volume for later use by using the ultra-pure water; or adding a certain amount of methylene dichloride, uniformly mixing through vortex, adding the ultra-pure water, ultrasonically oscillating, then loading on a centrifuge for centrifugal separation, taking the supernatant and keeping constant volume for later use by using the ultra-pure water; (2) enriching and purifying: taking the supernatant and collecting eluent through a water-phase filter membrane and an RP (Reversed Phase) column; and (3) loading on a machine for detection: taking the eluent and inputting into an ion chromatograph equipped with a post-column derivation device and an ultraviolet detector for detection.

The invention discloses a multifunctional high-flux automatic chromatography detector and applications thereof in the technical field of biomedical science. The chromatography detector includes a shell, a chromatographic system, a mechanical system, a detection system and an analysis system; the chromatographic system includes a chromatographic device and a chromatographic assembly; the side wallof the chromatographic device is provided with one or more channels, and the chromatographic assembly is installed in the channels; the mechanical system includes a pedestal, a motor, a photoelectricswitch and a clamping groove, wherein the clamping groove is used for installing the chromatographic device, and the photoelectric switch is used for locating the chromatographic assembly; the detection system includes a light source, a detector and a circuit board, and is used for capturing and detecting signals sent by the chromatographic assembly; the analysis system includes software and hardware equipment used for installing the software, and is used for the sorting, collecting, analyzing and storing the data; and the chromatography detector can be packaged by the shell. The chromatography detector can realize qualitative and quantitative detection based on the results of chromatography having principles of visible light, Raman, time-resolved fluorescence, fluorescence, chemiluminescence, up-conversion luminescence, etc.

The invention relates to a gas chromatography detection method used for detecting power transformer oil, adopting an analyzing system with one time sampling, parallel double columns, one time shunt and simultaneous detection of two detectors. An auxiliary loading gas way is added in a gas loading inlet pipe of a detection system. The regulation is implemented according to the air flow of a main loading gas way, the air flow of the auxiliary loading gas way and a needle valve which is added on the auxiliary loading gas way in a compulsory shunting way. Thus, a detected sample is shunted into two chromatographic columns which are connected with the main loading gas way and the auxiliary loading gas way in parallel for analyzing hydrocarbon compositions and H2, CO and CO2 compositions to finish the composition analysis of the transformer oil. A nickel reformer which can control temperature is added on the gas way after the chromatographic column separation of the auxiliary loading gas way and the detection of a thermal conducting detector. The auxiliary loading gas way and the main loading gas way reformed by the nickel reformer together enter a flame ionization detector through a tee for the detection. A hydrogen pipe from a hydrogen inlet to the left end inlet of the reformer in the auxiliary loading gas way is connected in parallel with a hydrogen pipe from an original hydrogen inlet to the flame ionization detector.

The invention relates to an ultrasonic countercurrent extraction method of polyprenol. At present, extraction methods of polyprenol with short extraction period, little solvent consumption and high extraction ratio do not exist. The invention successively comprises the following steps: (1) cleaning; (2) drying; (3) crushing; (4) putting crushed materials in a state with a temperature of 30 to 70 DEG C and an ultrasonic frequency of 20 to 60 KHz, and making the solvent move with the counter crushed materials with a relative velocity of 0.5 to 1.0 m / h; (5) carrying out vacuum concentration, cold separation, hydrolysis, phase separation, extraction, washing and reconcentration; (6) carrying out first column chromatography with a pressure of 0.5 to 2 bar to obtain crude polyprenol through collecting chromatography products at a wavelength of 215nm; and (7) carrying out second column chromatography with the pressure of 2 to 5 bar to obtain finished polyprenol through collecting the chromatography products at the wavelength of 215nm. The method has the advantages of short extraction period, little solvent consumption, high extraction ratio and the finished product purity of more than 90%.

A probe assembly is disclosed comprising an inlet for receiving an eluent from a chromatography device; an outlet (120) for delivering the eluent to an ion source of a mass spectrometer; and an attachment device (122) for attaching the outlet to the mass spectrometer. The outlet comprises an electrically conductive capillary (124) and an electrically conductive member (129) surrounding at least part of the electrically conductive capillary (124). The electrically conductive member (129) is arranged to receive a voltage upon connection of the attachment device (122) to the mass spectrometer and the electrically conductive member (129) is arranged to provide an electrical connection from the electrically conductive member (129) to the electrically conductive capillary (124).

Described herein is a method of adjusting the composition of a chromatography product to achieve a target enriched proportion of a desired component from an input feed having a lower proportion of the desired component using simulated moving bed (“SMB”) chromatography wherein the eluent for the SMB apparatus may comprise the very input feed being enriched. The method is exemplified by enriching a high fructose corn syrup from a 42% fructose syrup to a 55% fructose syrup without substantially reducing the dissolved solids concentration of the 55% syrup relative to the input 42% syrup. The 42% syrup is also used as the eluent for the SMB apparatus and may be reconstituted from the raffinate stream by passing the raffinate stream over a glucose isomerase column alone or in combination with a dextrose feed. The method reduces water usage and saves energy by minimizing the need for evaporation to obtain a 55% fructose syrup with a high dissolved solids content.

The invention discloses a high-performance-liquid-chromatography detection method for methylene blue in aquaculture water. The method comprises: pre-processing the aquaculture water, performing extraction by employing dichloromethane, performing vacuum-pumping condensation purification by employing a rotary evaporator, detecting by employing a liquid chromatogram-ultraviolet detector, and employing an external standard method to perform quantitative analysis. The method is convenient to operate, relatively good in repeatability, accurate in qualification and relatively high in recovery rate, and is capable of rapidly analyzing the methylene blue content in aquaculture water.

The invention discloses a detection method of aroma components of rubus biflorus fruits. The aroma components of the rubus biflorus fruits are detected by combining headspace solid-phase micro-extraction with gas chromatography-mass spectrometry. The detection method comprises the following steps of: inserting PA extraction fiber end into a sample feeding hole of a gas chromatograph-mass spectrometer and pre-treating; extracting volatile components by the headspace solid-phase micro-extraction; and carrying out qualitative and quantitative analysis on the aroma components of the rubus biflorus fruits by the gas chromatography-mass spectrometry. The detection method disclosed by the invention has the advantages of less use amount of samples, no need of utilizing solvents in an extraction process, rapid extraction speed, capability of directly carrying out chromatography detection on an extracted sample, simplicity and convenience of operation and high enriching efficiency. The aroma components of the rubus biflorus fruits can be simply, rapidly and accurately detected, and a foundation is laid for understanding flavor chemical constitution of the rubus biflorus fruits, guiding variety breeding of rubus biflorus, developing deep processing of the rubus biflorus fruits and controlling the product quality.

A chromatography column comprising a chromatography tube, a piston tube with a porous obstruction that inhibits the movement of particle solids therethrough, and a quick connect / disconnect clamp for use in coupling the chromatography tube to the piston tube. This column is for use in a liquid chromatography process where fluid is pumped under pressure through the chromatography tube which contains a packed particulate solid. The clamp allows for the quick removal and replacement of chromatography tubes when the particulate needs replacement.

This application discloses, in part, 1) a stationary phase column and compression designs for preparative chromatography, 2) a method of improving performance of silica gel chromatography by controlling the hydration of silica gel and acidifying the mobile phase, and 3) a method of extending the life of a silica gel column packing by cleaning or regenerating the silica gel stationary phase.

The chromatography method of the present invention includes (a) developing a complex of a test substance and a labeling substance modified with a first binding substance bindable to the test substance, onto an insoluble carrier, (b) causing the complex to be trapped in a reaction site on the insoluble carrier which contains a second binding substance bindable to the test substance or a substance exhibiting binding properties to the first binding substance, (c) washing the insoluble carrier with a washing solution containing at least one of the potassium iodide, urea, and guanidine after the step (b), (d) washing off the washing solution of the step (c) remaining in the insoluble carrier from the insoluble carrier, and (e) amplifying the labeling substance of the complex trapped in the reaction site.

The invention provides a column chromatography device with a warning function. The device solves the problem that the existing chromatography device cannot alarm when the liquid is too much or too little. The device comprises a column body, an upper end socket, a lower end socket and a liquid level alarm, wherein a filler is filled in the column body, the column body is provided with a filler inlet and a filler outlet, the upper end socket is arranged on the upper end of the column body and provided with a liquid inlet, an exhaust port and a back-flushing liquid outlet, the lower end socket is arranged at the lower end of the column body and provided with the liquid outlet, the liquid level alarm is arranged on the upper end socket, and the column body is fixedly connected with the upper end socket and the lower end socket. By arranging the liquid level alarm, the column chromatography device with the warning function provided by the invention is capable of providing the warning function when the liquid is too much or too little under the unwatched state, and the device is reasonable in structure, convenient to operate, low in equipment cost, and suitable for commercial popularization and application.

A chromatography method including a step of developing a complex of a specimen and a labeled substance modified by a first bondable substance which can be bonded to the specimen on an insoluble carrier, a step of capturing the complex of the specimen and the labeled substance at a reactive site on the insoluble carrier, a step of washing the reactive site on the insoluble carrier using a washing liquid, a step of detecting the specimen which is captured at the reactive site after a signal of the labeled substance is amplified by an amplification liquid, in which the reactive site on the insoluble carrier includes (i) a second bondable substance which can be bonded to the specimen or a substance having affinity to the first bondable substance which can be bonded to the specimen, and (ii) polyethylene glycol having the molecular weight from 1,500 to 10,000.

The invention discloses a device and a method for separating and purifying anthocyanidin. The device comprises a central distribution tank, and the feed inlet of the central distribution tank is respectively connected with an anthocyanin concentrate feeding tube, an eluent feeding tube and a pure water supply tube; a resin is arranged in a chromatography column, the top of the chromatography column is provided with an activation liquid inlet and a feed port, the bottom of the chromatography column is provided with a discharge main tube, and the feed port is communicated with the discharge portof the central distribution tank; the chromatography column is also provided with a resin replacement inlet and a resin replacement outlet; and the discharge man tube is respectively connected with an eluent storage tank, a byproduct storage tank, a high-purity liquid storage tank and a low-purity liquid storage tank, the discharge main tube is further provided with an activation liquid outlet joint and a sewage discharge outlet joint, the discharge main tube is provided with a liquid chromatography detector, and the activation liquid outlet joint is connected with the activation liquid storage tank. The separation and purification device can keep a high separation and purification efficiency for a long time, and is suitable for the automatic extraction process of anthocyanin.

The disclosed thin-layer chromatography (TLC) method for Sudan pigment in capsicum product comprises: extracting pigment by petroleum ether for power or by active clay for oil, desorbing the pigment by anhydrous alcohol, fixing volume by petroleum ether, and analyzing by TLC. This invention is convenient and low cost, overcomes defects in prior art, improves detection effect, and has bright application future.

The embodiment of the invention discloses a refilling type sulfur hexafluoride decomposition product chromatography online detector. The refilling type chromatography detector is used for single-hole sulfur hexafluoride electrical equipment, achieves automatic online detection and analysis of insulating gas of the sulfur hexafluoride electrical equipment in the modes of vacuumizing, sample collection, sample analysis and sample refilling, has multiple detection components and high detection precision and can achieve automatic sampling, detection and refilling of sampling gas of the sulfur hexafluoride electrical equipment, so that harm caused by gas emission to personnel and environment is avoided, the insulativity of the electrical equipment cannot be affected by gas refilling, and the online detection frequency and timeliness of sulfur hexafluoride are greatly improved.