Multifunctional high-flux automatic chromatography detector and applications thereof

A detector and high-throughput technology, applied in the field of biomedicine, can solve the problems of complex amplification and filtering circuits, inability to respond to signals, and increase the complexity of the system, achieving broad market prospects, small size, and easy portability.

Active Publication Date: 2018-10-19
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the wide application of chromatography detection methods in many fields such as medical treatment, food, medicine, chemistry and forensic identification, some chromatography detection devices have also appeared on the market, but these devices can only be used for the chromatography results of one principle. Detection, such as the results of colloidal gold immunochromatographic test strips, can only be read, and cannot respond to signals such as Raman, time-resolved fluorescence, chemiluminescence, and up-conversion luminescence
[0004] The invention patent with the application number of 201310045753X discloses a quantitative detection system and detection method of a multi-channel test paper tray. It consists of amplification and filtering circuit, control, data acquisition and processing device, and its signal detector adopts photodetector component. In order to collect the output signal of photodetector component, it also needs complex amplification and filtering circuit system, which increases the The complexity of the whole system
The invention patent with the application number 2010105911120 discloses a quantitative detector for the color signal of the immunochromatographic detection test strip, including a light source, an opaque casing, a conversion lens, a slit, a photodetector, a primary amplifier circuit and an AD control circuit Composed of communication interface, it has the same problem as the invention patent with application number 201310045753X, and also requires complex circuit system

Method used

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  • Multifunctional high-flux automatic chromatography detector and applications thereof
  • Multifunctional high-flux automatic chromatography detector and applications thereof
  • Multifunctional high-flux automatic chromatography detector and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1: Multifunctional high-throughput automated chromatography detector

[0040] A multi-functional high-throughput automatic chromatography detector, such as figure 1 As shown, the chromatography detector includes a shell, a chromatography system, a mechanical system, a detection system and an analysis system; wherein,

[0041] The chromatographic system includes a chromatographic device 15 and a chromatographic assembly 8. The chromatographic device 15 is a hollow pipe whose side wall is a ring. In another embodiment, its side wall is polyhedral; its side wall Four channels 1 along the length of the pipeline are arranged on the wall, and in another embodiment, the number of the channels 1 is set to 1, 2, 4, 8, 12 or 24; a chromatography component is installed in each channel 1 8; The chromatography device 15 is provided with a sample injection port 7 .

[0042] Described mechanical system comprises base 5, motor 4, photoelectric switch 3 and draw-in slot 2, and...

Embodiment 2

[0062] Example 2: Multifunctional high-throughput automated chromatography detector

[0063] The difference between this embodiment and Embodiment 1 is that the chromatographic component 8 is a Raman signal immunochromatographic test strip, the chromatographic device is provided with 4 channels, and the detector 6 is a Raman spectrometer.

[0064] The above-mentioned multifunctional high-throughput automatic chromatography detection instrument differs from Example 1 in its operation steps in that step (7) is: open the detection function of the Raman spectrometer, and set the parameters of the Raman spectrometer by executing the software on the mobile phone or computer , such as laser power, integration time, etc., point-by-point detection of enhanced Raman-based immunochromatography results; automatic analysis and interpretation of enhanced Raman immunochromatography detection results, and print the interpretation results.

Embodiment 3

[0065] Example 3: Detection of procalcitonin (PCT), interleukin-6 (IL-6) and C-reactive protein (CRP) in a single sample

[0066] Materials: CRP, PCT, IL6 colloidal gold chromatography system; adopt the multifunctional high-throughput automatic chromatography detector similar to that described in Example 1 of the present invention, the difference is that the chromatography device is provided with three channels; human serum One sample (provided by the Laboratory Department of Beijing 301 Hospital).

[0067] The operation steps are as follows:

[0068] (1) Prepare CRP, PCT, IL6 colloidal gold immunochromatography test strips, respectively pack the colloidal gold immunochromatography test strips into different channels of the chromatography device, and pack the chromatography device into the card slot;

[0069] The preparation method of CRP, PCT, IL6 colloidal gold immunochromatographic test strips is as follows: colloidal gold is electrostatically adsorbed to modify the monocl...

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Abstract

The invention discloses a multifunctional high-flux automatic chromatography detector and applications thereof in the technical field of biomedical science. The chromatography detector includes a shell, a chromatographic system, a mechanical system, a detection system and an analysis system; the chromatographic system includes a chromatographic device and a chromatographic assembly; the side wallof the chromatographic device is provided with one or more channels, and the chromatographic assembly is installed in the channels; the mechanical system includes a pedestal, a motor, a photoelectricswitch and a clamping groove, wherein the clamping groove is used for installing the chromatographic device, and the photoelectric switch is used for locating the chromatographic assembly; the detection system includes a light source, a detector and a circuit board, and is used for capturing and detecting signals sent by the chromatographic assembly; the analysis system includes software and hardware equipment used for installing the software, and is used for the sorting, collecting, analyzing and storing the data; and the chromatography detector can be packaged by the shell. The chromatography detector can realize qualitative and quantitative detection based on the results of chromatography having principles of visible light, Raman, time-resolved fluorescence, fluorescence, chemiluminescence, up-conversion luminescence, etc.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a multifunctional high-throughput automatic chromatography detector and its application. Background technique [0002] Chromatography is the abbreviation of "chromatographic analysis". It is a method of separating and measuring a multi-component mixture by using different properties of each component, using different separation media such as silica gel and detection instruments. Taking the commonly used immunochromatography as an example, the principle is to immobilize the specific antibody on a specific zone of the nitrocellulose membrane, and then add the sample dropwise to one end of the membrane. Under the action of the capillary, the sample is immediately Moving forward along the membrane, when moving to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antibody. At this time, if immunological n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
CPCG01N35/00
Inventor 王升启肖瑞荣振吴凤池王娈娈张敏丽王琼
Owner ACADEMY OF MILITARY MEDICAL SCI
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