High-performance-liquid-chromatography detection method for methylene blue in aquaculture water

A high-performance liquid chromatography and methylene blue technology, which is applied in the field of high-performance liquid chromatography detection of methylene blue in aquaculture water, can solve problems such as being unsuitable for detection of methylene blue in aquaculture water, and achieves low detection cost, accurate qualitative, high sensitivity and precision. Effect

Active Publication Date: 2014-08-13
MARINE FISHERIES RES INST OF ZHEJIANG
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The published detection methods for methylene blue in aquatic products include the indirect iodometric method and spectrophotometric method stipul

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-performance-liquid-chromatography detection method for methylene blue in aquaculture water
  • High-performance-liquid-chromatography detection method for methylene blue in aquaculture water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Water sample pretreatment: After collecting 500 mL of water sample to the laboratory, immediately use a 0.45 μm filter membrane for suction filtration treatment, and transfer the filtrate obtained by suction filtration to a sample bottle.

[0027] (2) Extraction and purification: Select a 250 mL separatory funnel for leak detection and place it on the funnel stand, accurately measure 100 mL of the filtrate and transfer it to a 250 mL separatory funnel, add 40 mL of chromatographically pure dichloromethane for extraction, shake for 1 min and then statically After standing for 20 min, when the liquid in the separatory funnel was stable and obvious stratification appeared, the dichloromethane in the lower layer was transferred to a 100 mL rotary evaporator, and concentrated to dryness in a rotary evaporator at 40 °C under vacuum. After removing the rotary evaporator, accurately add 1.0 mL of methanol to the rotary evaporator to dissolve the residue, and use a rubber-tip...

Embodiment 2

[0035] (1) Water sample pretreatment: After collecting 1000 mL of water sample to the laboratory, immediately use a 0.45 μm filter membrane for suction filtration treatment, and transfer the filtrate obtained by suction filtration to a sample bottle.

[0036] (2) Extraction and purification: Select a 500 mL separatory funnel for leak detection and place it on the funnel stand, accurately measure 250 mL of the filtrate and transfer it to a 500 mL separatory funnel, add 50 mL of chromatographically pure dichloromethane for extraction, shake for 2 minutes and then let it stand After 30 min, when the liquid in the separatory funnel was stable and obvious stratification appeared, the dichloromethane in the lower layer was transferred to a 100 mL rotary evaporator, and concentrated to dryness in a rotary evaporator at 45 °C under vacuum. After removing the rotary evaporator, accurately add 1.0 mL of methanol to the rotary evaporator to dissolve the residue, and use a rubber-tipped st...

Embodiment 3

[0044] (1) Water sample pretreatment: After collecting 1000 mL of water sample to the laboratory, immediately use a 0.45 μm filter membrane for suction filtration treatment, and transfer the filtrate obtained by suction filtration to a sample bottle.

[0045] (2) Extraction and purification: Select a 500 mL separatory funnel for leak detection and place it on the funnel stand, accurately measure 250 mL of the filtrate and transfer it to a 500 mL separatory funnel, add 60 mL of chromatographically pure dichloromethane for extraction, shake for 1 min and then statically After standing for 20 min, when the liquid in the separatory funnel was stable and obvious stratification appeared, the dichloromethane in the lower layer was transferred to a 100 mL rotary evaporator, and concentrated to dryness in a rotary evaporator at 40 °C under vacuum. After removing the rotary evaporator, accurately add 1.0 mL of methanol to the rotary evaporator to dissolve the residue, and use a rubber-ti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention discloses a high-performance-liquid-chromatography detection method for methylene blue in aquaculture water. The method comprises: pre-processing the aquaculture water, performing extraction by employing dichloromethane, performing vacuum-pumping condensation purification by employing a rotary evaporator, detecting by employing a liquid chromatogram-ultraviolet detector, and employing an external standard method to perform quantitative analysis. The method is convenient to operate, relatively good in repeatability, accurate in qualification and relatively high in recovery rate, and is capable of rapidly analyzing the methylene blue content in aquaculture water.

Description

technical field [0001] The invention relates to the technical field of environmental quality and safety detection, in particular to a high performance liquid chromatography detection method for methylene blue in aquaculture water. Background technique [0002] Methylene blue belongs to a chemical indicator, industrial dye, biological dye and drug, and its toxicity is lower than that of malachite green. In recent years, it has been widely used in aquatic product cultivation as a substitute for banned drugs such as malachite green and nitrofuran It can prevent streptochytridosis, saprolegniasis and parasitic diseases caused by fungi. It is often used in aquaculture to treat saprolegniasis and to disinfect the environment of aquaculture water. Extensive use or abuse of methylene blue will have certain toxic and side effects on aquaculture aquatic organisms, and it is difficult to control the concentration of the drug in actual production, resulting in the accumulation of a cert...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 龙举龙位张小军李佩佩严忠雍何依娜
Owner MARINE FISHERIES RES INST OF ZHEJIANG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap