Fluorescent test paper capable of rapidly detecting formaldehyde and application of fluorescent test paper

Abstract

The invention discloses fluorescent test paper capable of rapidly detecting formaldehyde. The fluorescent test paper is formed by fluorescent-whitening-agent-free test paper loaded with fluorescent probe naphthalimides derivative. The fluorescent test paper is deep green, the size of the test paper is (100-75)mm*(15-8)mm*(1-0.5)mm, the content of the naphthalimides derivative loaded on each fluorescent test paper is not less than 0.01mg, the test paper for a substrate is deep-green precision pH test paper, and the naphthalimides derivative loaded on the test paper for the substrate is N-amino-4-(1-piperazinyl)-1,8-naphthalimide, N-amino-4-(1-piperazinyl)-1,8-naphthalimid or N-amino-4-(1-pyrrolidinyl)-1,8-naphthalimide. The fluorescent test paper is easy to prepare, convenient to use and capable of rapidly and accurately detecting and determining the formaldehyde in the environment.

Description

technical field

[0001] The invention relates to a fluorescent test paper for rapidly detecting formaldehyde and an application thereof, belonging to the technical field of analytical chemistry. Background technique

[0002] Formaldehyde is a colorless, irritating gas that is easily soluble in water and can be absorbed by the human body through the respiratory tract. When the formaldehyde content in the indoor air is 0.1mg / m 3 Odor and discomfort; 0.5mg / m 3 Sometimes it can irritate the eyes and cause tears; 0.6mg / m 3 When the concentration is higher, it can cause nausea, vomiting, cough, chest tightness, asthma and even emphysema; and when the formaldehyde in the air reaches 30mg / m 3 can result in immediate death. Long-term exposure to low-dose formaldehyde can cause chronic respiratory diseases, reduced physical fitness of newborns, chromosomal abnormalities, and even nasopharyngeal cancer. High concentrations of formaldehyde are toxic to the nervous system, immune sys...

Examples

Embodiment 1

[0033] Example 1: Preparation of N-amino-4-(1-piperazinyl)-1,8-naphthalimide

[0034] Add hydrazine hydrate (80%, 62.5mg, 1mmol), 4-bromo-1,8-naphthalic anhydride (276mg, 1mmol) and ethanol (10mL) into a 50mL single-necked flask, reflux for 4 hours, and cool to room temperature Filter and dry to obtain 240mg of light gray solid. Add this solid, anhydrous piperazine (258mg, 3mmol) and ethylene glycol monomethyl ether (10mL) into a 50mL one-necked flask, reflux for 6 hours under nitrogen protection, cool to room temperature and distill under reduced pressure to obtain a dark brown viscous solid .

[0035] The sticky solid was purified by column chromatography (eluant: methanol containing 5% triethylamine) to give a yellow solid. The obtained yellow solid, 10 mL of distilled water, and 5 mL of methanol were added into a 50 mL single-necked flask, stirred at room temperature for 24 hours, filtered, and dried to obtain a light yellow product with a yield of 67%. The probe's 1 H...

Embodiment 2

[0037] Embodiment 2: the preparation of the fluorescent test paper of rapid detection formaldehyde of the present invention

[0038] (1) Using methanol as a solvent to prepare a solution of N-amino-4-(1-piperazinyl)-1,8-naphthalimide with a concentration of 1 mM;

[0039] (2) Soak dark green precision pH test paper (length × width × thickness is 75mm × 10mm × 0.7mm) in the solution prepared in step (1), take it out after 10 minutes, and dry it at room temperature to obtain dark green color. Fluorescent test paper that can quickly detect formaldehyde without fluorescence under ultraviolet light (see figure 2 ).

Embodiment 3

[0040] Embodiment 3: the selectivity of N-amino-4-(1-piperazinyl)-1,8-naphthalimide to different substances

[0041] Prepare 8 parts of 5 mL of 10 μM N-amino-4-(1-piperazinyl)-1,8-naphthoimide probe acetonitrile solution (containing 5% acetic acid), and then add to the 8 parts of the system in sequence 50 µL of formaldehyde, acetaldehyde, 2,4-dihydroxybenzaldehyde, terephthalaldehyde, p-methoxybenzaldehyde, chloral, m-hydroxybenzaldehyde, and p-hydroxybenzaldehyde in acetonitrile at a concentration of 10 mM. Fluorescence detection was then performed (λ Ex =380nm,λ Em =513nm); Calculate the fluorescence intensity in each system, see the results image 3 .

technical field

[0001] The invention relates to a fluorescent test paper for rapidly detecting formaldehyde and an application thereof, belonging to the technical field of analytical chemistry. Background technique

[0002] Formaldehyde is a colorless, irritating gas that is easily soluble in water and can be absorbed by the human body through the respiratory tract. When the formaldehyde content in the indoor air is 0.1mg / m 3 Odor and discomfort; 0.5mg / m 3 Sometimes it can irritate the eyes and cause tears; 0.6mg / m 3 When the concentration is higher, it can cause nausea, vomiting, cough, chest tightness, asthma and even emphysema; and when the formaldehyde in the air reaches 30mg / m 3 can result in immediate death. Long-term exposure to low-dose formaldehyde can cause chronic respiratory diseases, reduced physical fitness of newborns, chromosomal abnormalities, and even nasopharyngeal cancer. High concentrations of formaldehyde are toxic to the nervous system, immune sys...

Examples

Embodiment 1

[0033] Example 1: Preparation of N-amino-4-(1-piperazinyl)-1,8-naphthalimide

[0034] Add hydrazine hydrate (80%, 62.5mg, 1mmol), 4-bromo-1,8-naphthalic anhydride (276mg, 1mmol) and ethanol (10mL) into a 50mL single-necked flask, reflux for 4 hours, and cool to room temperature Filter and dry to obtain 240mg of light gray solid. Add this solid, anhydrous piperazine (258mg, 3mmol) and ethylene glycol monomethyl ether (10mL) into a 50mL one-necked flask, reflux for 6 hours under nitrogen protection, cool to room temperature and distill under reduced pressure to obtain a dark brown viscous solid .

[0035] The sticky solid was purified by column chromatography (eluant: methanol containing 5% triethylamine) to give a yellow solid. The obtained yellow solid, 10 mL of distilled water, and 5 mL of methanol were added into a 50 mL single-necked flask, stirred at room temperature for 24 hours, filtered, and dried to obtain a light yellow product with a yield of 67%. The probe's 1 H...

Embodiment 2

[0037] Embodiment 2: the preparation of the fluorescent test paper of rapid detection formaldehyde of the present invention

[0038] (1) Using methanol as a solvent to prepare a solution of N-amino-4-(1-piperazinyl)-1,8-naphthalimide with a concentration of 1 mM;

[0039] (2) Soak dark green precision pH test paper (length × width × thickness is 75mm × 10mm × 0.7mm) in the solution prepared in step (1), take it out after 10 minutes, and dry it at room temperature to obtain dark green color. Fluorescent test paper that can quickly detect formaldehyde without fluorescence under ultraviolet light (see figure 2 ).

Embodiment 3

[0040] Embodiment 3: the selectivity of N-amino-4-(1-piperazinyl)-1,8-naphthalimide to different substances

[0041] Prepare 8 parts of 5 mL of 10 μM N-amino-4-(1-piperazinyl)-1,8-naphthoimide probe acetonitrile solution (containing 5% acetic acid), and then add to the 8 parts of the system in sequence 50 µL of formaldehyde, acetaldehyde, 2,4-dihydroxybenzaldehyde, terephthalaldehyde, p-methoxybenzaldehyde, chloral, m-hydroxybenzaldehyde, and p-hydroxybenzaldehyde in acetonitrile at a concentration of 10 mM. Fluorescence detection was then performed (λ Ex =380nm,λ Em =513nm); Calculate the fluorescence intensity in each system, see the results image 3 .

Images