Thin-layered chromatography detection method for Sudan pigment in red pepper products

A detection method, thin-layer chromatography technology, is applied in the field of detecting whether Sudan pigments are contained in pepper products, and can solve problems such as difficult to meet the production and sales site management of pepper products, backward equipment in the basic inspection department, and few effective inspection methods, etc., to achieve Good application prospects, overcoming the effect of high requirements for instruments and low requirements for instruments and equipment

Inactive Publication Date: 2007-03-14
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In March 2005, Sudan dye was detected for the first time as an illegal food additive in chili products in my country, but relevant effective inspection methods are few and cannot be popularized
At present, there are no other methods in China, and the main disadvantages of these methods are complicated operations and high requirements for equipment conditions, which are not conducive to popularization in the environment where the equipment of the majority of grass-roots testing departments in China is backward and the quality of operators is generally low, and it is difficult to meet the requirements for pepper products. Production and sales site management needs

Method used

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  • Thin-layered chromatography detection method for Sudan pigment in red pepper products
  • Thin-layered chromatography detection method for Sudan pigment in red pepper products
  • Thin-layered chromatography detection method for Sudan pigment in red pepper products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Step 1: Weigh 2.8 grams of commercially available chili powder into a beaker, add 10ml of petroleum ether, mix on a vortex mixer for 3 minutes, then let stand for 30 minutes until the insoluble matter is completely precipitated, pour out the supernatant, and then repeat the above operation 2 until the color of the chili lightens.

[0022] Step 2: Mix the above-mentioned extracted liquids, evaporate to dryness, add 100ml petroleum ether to dissolve and set aside.

[0023] Step 3: Weigh 0.05 g of Sudan I pigment and place it in a 100ml volumetric flask to dilute to volume with petroleum ether to prepare a 0.5 g / L Sudan I pigment standard solution.

[0024] Step 4: Take 3 μl of the standby sample prepared in step 2 and the Sudan I pigment standard solution prepared in step 3 and place samples on the silica gel thin-layer plate, and the composition of the silica gel thin-layer plate is Si60F 254 , produced by MERCK GERMANY. It is developed in a developing agent, which is ...

Embodiment 2

[0027] Step 1: Weigh 2.97 grams of the same bottle of commercially available chili powder in Example 1 into a beaker, add 0.11 grams of Sudan I and mix thoroughly, then add 10ml of petroleum ether, mix on a vortex mixer for 3 minutes, and then let stand for 30 minutes until insoluble The substance is completely precipitated, and the supernatant is poured out, and then the above operation is repeated 2 times until the color of the pepper becomes lighter.

[0028] Step 2: Mix the above-mentioned extracted liquids, evaporate to dryness, add 250ml petroleum ether to dissolve and set aside.

[0029] Step 3: Weigh 0.05 g of Sudan I pigment and place it in a 100ml volumetric flask to dilute to volume with petroleum ether to prepare a 0.5 g / L Sudan I pigment standard solution.

[0030] Step 4: Take 5 μl of the standby sample prepared in step 2 and the Sudan I pigment standard solution prepared in step 3 and place samples on the silica gel thin-layer plate, and the composition of the s...

Embodiment 3

[0033] Step 1: ①. Weigh 2.8 grams of the same bottle of commercially available chili powder in Example 1 into a beaker, add 10ml of petroleum ether, mix it on a vortex mixer for 3 minutes, and then let it stand for 30 minutes until the insoluble matter is completely precipitated. Clear liquid, and then repeat the above operation 2 times until the color of pepper lightens. 2. Weigh 2.97 grams of the same bottle of commercially available chili powder in Example 1 in a beaker, add 0.11 grams of Sudan I and mix thoroughly, then add 10 ml of petroleum ether, mix on a vortex mixer for 3 minutes, and then let stand for 30 minutes until insoluble matter Complete precipitation, pour out the supernatant, and then repeat the above operation 2 times until the color of the pepper becomes lighter.

[0034] Step 2: Mix the liquid extracted in step 1.1, evaporate to dryness, add 200ml of petroleum ether to dissolve and make sample 1 for later use. Mix the liquids extracted in step 1.2, evapo...

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Abstract

The disclosed thin-layer chromatography (TLC) method for Sudan pigment in capsicum product comprises: extracting pigment by petroleum ether for power or by active clay for oil, desorbing the pigment by anhydrous alcohol, fixing volume by petroleum ether, and analyzing by TLC. This invention is convenient and low cost, overcomes defects in prior art, improves detection effect, and has bright application future.

Description

technical field [0001] The invention relates to a method for detecting whether Sudan pigment is contained in chili products, in particular to a thin-layer chromatography detection method for Sudan pigment in chili powder and chili oil. Background technique [0002] Sudan pigments are lipophilic azo compounds, mainly including four types: I, II, III and IV. The Sudan pigments that enter the body are mainly metabolized by the reductases of the gastrointestinal tract microorganisms, microsomes and cytoplasm of the liver and extrahepatic tissues, and are metabolized into corresponding amines in the body. In a number of in vitro mutagenic tests and animal carcinogenic tests, it was found that the mutagenicity and carcinogenicity of Sudan pigment are related to the amines produced by metabolism. Sudan pigment is a kind of artificially synthesized industrial dye. In 2003, the European Union (EU) and other countries have banned it from being added as a pigment in food, and our coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/28
Inventor 丁长河钱林任惠华陈复生李里特
Owner HENAN UNIVERSITY OF TECHNOLOGY
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