33results about How to "Suitable for separation and purification" patented technology

A process and apparatus for pressure purifying of trichlorosilane is disclosed. Said process includes such steps as distilling the mixed liquid of trichlorosilane, SiCl4 and chlorosilane at 70-200 deg.C under 0.15-1.5 MPa, sending the vapor derived from distilled into purifying tower for the exchange and separation of heat and components at 40-150 deg.C, enriching the low-boiling-point trichlorosilane in vapor phase and the high-boiling-point SiCl4 in liquid phase, evaporating and condensaing several times to obtain trichlorosilane vapor, and condensing to obtain liquid-phase trichlorosilane.

The invention relates to an online low-pressure, middle-pressure and high-pressure combined two-dimension preparation liquid chromatographic system. Two one-dimension low-pressure, middle-pressure and high-pressure preparation liquid chromatographic systems are organically combined through a six-way valve and software. Pressure used by a second dimension system is not lower than pressure used by a first dimension separation system. When the first dimension system and the second dimension system are low-pressure or middle-pressure preparation liquid chromatographic systems, through switching the six-way valve, target compound directly enters a second dimension chromatographic column for second dimension separation after first dimension separation; and when pressure used by a second dimension separation system is higher than pressure used by the first dimension separation system, a trapping column is connected on the six-way valve, the target compound is adsorbed on the head of the trapping column after the first dimension separation, and then the target compound enters the second dimension chromatographic column for second dimension separation. The invention has the advantages that the contradiction between sample injection volume and separation capacity is solved, the large sample injection volume is ensured and the separation capacity of the preparation chromatographic system is greatly improved; the system structure is simple and the establishment is easy; the operation is simple and convenient and the automation can be easily realized; and the application scope is wide.

The invention discloses a novel method for preparing macroporous silicon dioxide@porous polymer core-shell microspheres. Non-porous silica microspheres are used as a core, octadecyltrichlorosilane isused for performing the surface hydrophobic modification, PVA and SDS are used as a dispersing agent, monomers GMA, a cross-linking agent EDMA and a pore forming agent are added to prepare the silicondioxide@porous polymer core-shell microspheres with a macroporous structure through an emulsion polymerization process. The preparation method is simple in preparation process, mild in conditions, and easy in mass production; and the surface of the prepared core-shell microspheres is a porous organic polymer which has higher acid and alkali resistance, the application range of core-shell fillersis enlarged, a larger aperture structure can be obtained, and the prepared core-shell microspheres are particularly suitable for the isolated analysis of biological molecules.

Belonging to the technical field of genetic engineering recombinant protein purification, the invention relates to a method for purification of recombinant protein. The method includes: 1) centrifuging a fermentation broth to obtain a supernatant, and performing ultrafiltration and concentration on the supernatant; 2) subjecting the ultrafiltered concentrated solution to gel filtration chromatography to remove pigment and salt; 3) conducting anion chromatography on the obtained liquid, performing high salt gradient elution, and collecting a target protein peak; and 4) subjecting the collected liquid to hydrophobic chromatography to obtain pure recombinant human apolipoprotein ApoA-I. The method provided by the invention is employed to purify recombinant human apolipoprotein Apoa-I, the fermentation supernatant is subjected to ultrafiltration and concentration, further gel chromatography and hydrophobic chromatography are adopted, the final eluent is water, and can be directly subjected to freeze drying without desalination, the obtained recombinant human ApoA-I has purity of more than 96%, the recovery rate is higher than 82%, and the treatment capacity for each time is 3L. The method has the advantages of simplicity and convenience, stable process and easy amplification, can solve the scale problem in industrialization production of recombinant human apolipoprotein ApoA-I, and is suitable for separation and purification of production.

The invention provides a multi-channel comprehensive two-dimensional high-performance liquid chromatograph with a multifunctional switching valve. The multi-channel comprehensive two-dimensional high-performance liquid chromatograph comprises a one-dimensional chromatographic separation system and a multi-channel two-dimensional chromatographic separation system and has the advantages that by the aid of a multi-channel two-dimensional separation system, two-dimensional separation is achieved by the aid of multiple external chromatographic columns, selectable types of stationary phases are more, separation efficiency is high, separation effect is good, total components of complicated samples can be separated and analyzed, mobile phases of each channel are independently controlled, separation processes can be accurately controlled, separation effect is improved, two-dimensional separation is achieved by the aid of a plurality of two-dimensional chromatographic columns, limit of the size of one-dimensional chromatographic columns and flow speed of the mobile phases is omitted, and the chromatograph can be used for preparative separation of large samples.

The invention discloses a method for extracting, purifying, and inspecting astragalus in Beiqi mushrooms, and relates to the field of extraction, purification, and inspection of active substances in the Beiqi mushrooms. The method comprises the following steps of: taking 70% of ethanol as an extracting agent, and carrying out auxiliary treatment through a microwave ultrasonic auxiliary extractor to obtain an astragalus crude extracting solution; taking a mixed solution of chloroform, methanol, ethyl acetate, and water as a developing solvent, and separating and purifying astragaloside, astragalus saponin I, II, and III through thin-layer chromatography; using chromatography methanol and ultrapure water as a mobile phase, and accurately determining sample molecular mass information (a mass-to-charge ratio of molecular ions or fragment ions) with a high resolution mass spectrometer; detecting a functional group of the sample by infrared, and analyzing the molecular structure of the sample; and determining a rotation degree, a specific rotation degree, and a melting point of the sample. According to the method for extracting, purifying, and inspecting astragalus in Beiqi mushroom, microgram amount of separation analysis to gram amount of separation preparation can be achieved by using TLC-MS-IR series technology to detect astragalus in the Beiqi mushrooms; and the method is simpleto operate, short in time and high in efficiency.

The invention relates to the technical field of preparation of silane coupling agents, in particular to a preparation process of a phenylcarbamido silane coupling agent. According to the preparation method, (3-chloropropyl) diethoxymethylsilane and organic amine are used as raw materials for preparing a reactant, the prepared reactant and N-methyl-2-pyrrolidone are subjected to a chemical reaction under the action of a mixed solution composed of a phenyl isocyanate compound and N-methyl-2-pyrrolidone, and finally, a silane coupling agent crude product with high yield and high purity is generated. Not only is the waste of reaction raw materials effectively reduced, but also the purity of the finally prepared phenylcarbamido silane coupling agent is ensured. Then, separation and purification are carried out through an efficient adsorbent, so that the purity of the finally prepared phenylcarbamido silane coupling agent is higher, and the quality of the prepared phenylcarbamido silane coupling agent is effectively guaranteed.

The invention provides a comprehensive two-dimensional high performance liquid chromatograph provided with a multifunctional changeover valve. The comprehensive two-dimensional high performance liquid chromatograph provided with the multifunctional changeover valve comprises a one-dimensional chromatography mobile-phase liquid storage device M1, a one-dimensional chromatography high-pressure pump P1, a one-dimensional chromatography sampling valve V1, a one-dimensional chromatographic column C1, a one-dimensional chromatography detector D1, a two-dimensional chromatography mobile-phase liquid storage device M2, a two-dimensional chromatography high-pressure pump P2, the multifunctional changeover valve V, multiple two-dimensional separation columns C2-1 to C2-8, a two-dimensional chromatography detector D and a fraction collector F. The comprehensive two-dimensional high performance liquid chromatograph has the outstanding effects as follows: 1, two-dimensional separation is performed by use of multiple two-dimensional chromatographic columns, a fixed phase has multiple selectable varieties, the separation efficiency is high, the separation effect is good, and full-ingredient separation and analysis of complicated samples can be realized; 2, the system is provided with multiple detectors, accurate control of the separation process can be guaranteed, and the separation effect can be improved.

The invention relates to a separation and purification method for bilobalide monomers in a ginkgo leaf extract. The separation and purification method comprises the following steps: by taking the ginkgo leaf extract as a raw material, performing extraction and pretreatment, separating polyamide resin, then purifying with a PRP512C chromatographic packing, and finally performing recrystallization to obtain the bilobalide monomers with the purity of 95% or more, wherein the yield of bilobalide is 80% or more. A method for purifying the bilobalide monomers, which is simple and easy to operate, is established.