A method for separating and purifying plant leaf anthocyanins by using mixed adsorption resin

A technology for separation, purification, and resin adsorption, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems that anthocyanins and impurities are difficult to be completely separated, affecting the quantity and purity of anthocyanins, etc. The effect of short static desorption time, prevention of re-adsorption and high stability

Active Publication Date: 2021-07-16
河南省林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The impurities contained in the crude anthocyanin extract are mainly hydrophobic components, and the relatively hydrophilic anthocyanins usually use non-polar or medium-polar macroporous resin as the purification medium, which satisfies the requirements to different extents. When the adsorption force of anthocyanins, the resin has a stronger adsorption force on impurities, which makes it difficult to completely separate anthocyanins and impurities during the desorption process, thus affecting the quantity and purity of anthocyanins obtained

Method used

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  • A method for separating and purifying plant leaf anthocyanins by using mixed adsorption resin
  • A method for separating and purifying plant leaf anthocyanins by using mixed adsorption resin
  • A method for separating and purifying plant leaf anthocyanins by using mixed adsorption resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 preparation of anthocyanin extract

[0037] Take the fresh leaves of Populus chinensis, wash them with distilled water, and dry them. After removing the petiole and veins, cut it into pieces, weigh 3.0g of the sample in a mortar, add 10mL of the extract solution of absolute ethanol: water: HCl = 2:1:1, fully grind until homogenized, transfer to a 50mL test tube, add Dilute the extract to 30mL, shake it well for 1min, and extract it twice with ultrasound, 20min each time. Then bathe in boiling water for 1 hour, take it out and cool it down, dilute it to 50mL with 60% ethanol solution by volume fraction, let it stand still, take the supernatant, filter it with a 0.45μm filter membrane, and obtain the crude extract solution of anthocyanins from poplar leaves.

Embodiment 2

[0038] The optimization of embodiment two macroporous resin models

[0039] According to the preparation method of anthocyanin extract in Example 1, 300 mL of anthocyanin crude extract from poplar leaves were prepared, and refrigerated for future use. Usually, anthocyanin has a maximum absorption peak at 520-560nm in the visible light region. First, use a visible spectrophotometer to scan in the range of 400-700nm to measure the absorption wavelength of anthocyanin. The steps are as follows: take 2mL of the crude extract and dilute to 10mL with 60% ethanol solution by volume fraction, and use the ethanol solution with 60% volume fraction as the control, immediately use a visible spectrophotometer to scan in the range of 400-700nm to determine the concentration of the sample solution. The maximum absorption wavelength of anthocyanins is 531nm.

[0040] Choose non-polar HPD700, HPD100, X-5, weak polarity AB-8, medium polarity XAD-7, XAD-8, HPD500, NKA-9 these 8 kinds of macropo...

Embodiment 3

[0049] Example 3 The preferred mixing ratio of HPD700 and NKA-9

[0050] According to the preparation method of the anthocyanin extract in Example 1, 200 mL of the crude extract of anthocyanin from poplar leaves was prepared, and refrigerated for future use.

[0051] Take the processed macroporous resins NKA-9 and HPD700 respectively, mix them uniformly according to different mass ratios, weigh 1.0g of each mixed resin, put them in a 50mL conical flask with a stopper, add the leaves of Populus fragrans 30mL crude extract of pigment glycosides; shake in a water bath at 25°C for 2h at a speed of 100r / min, and receive the filtrate; put the filtered resin into 20mL of ethanol solution with a volume fraction of 60%, shake in a water bath at 100r / min for 3h at 25°C , suction filtration, and receive the filtrate; respectively measure the absorbance at 531 nm of the anthocyanin crude extract, the filtrate after adsorption and desorption, and calculate the adsorption capacity, adsorpti...

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Abstract

The invention belongs to the technical field of separation and purification of effective components of plant leaves, and in particular relates to a method for separating and purifying plant leaf anthocyanins with mixed adsorption resins. The present invention mixes non-polar and medium-polar resins according to a certain mass ratio, and adjusts the hydrophobic force of the resin by regulating the content of different polar components in the resin skeleton, thereby weakening the adsorption force of the resin and anthocyanin, and at the same time Without affecting the adsorption capacity of the resin to impurities, a mixed macroporous resin filter body suitable for the separation and purification of plant leaf anthocyanins is finally formed.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of effective components of plant leaves, and in particular relates to a method for separating and purifying plant leaf anthocyanins with mixed adsorption resins. Background technique [0002] As the most important and widely distributed colored substance in plants, anthocyanins are the material basis of almost all colors in plant petals, leaves and fruits. Anthocyanins have the basic structure of flavonoids, that is, (C6-C3-C6)C15 compounds composed of two aromatic rings and an oxygen-containing heterocycle, which have the following structure: [0003] [0004] According to the position and number of hydroxylation and methylation of the B ring, anthocyanins are mainly divided into six categories: when R1=R2=H, it is pelargonidin; when R1=OH, R2=H, it is cornflower Pigment (cyanidin); When R1=OMe, R2=H, it is peonidin (peonidin) (3'-methylcyanidin); when R1=R2=OH, it is delp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H17/065
CPCC07H1/08C07H17/065
Inventor 杨淑红张江涛朱镝高福玲董玉山赵蓬晖马永涛晏增徐惠鸽田丽
Owner 河南省林业科学研究院
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