The invention discloses application of a PhCHS-R1
gene in regulating and controlling the color of
plant petals, and belongs to the field of
plant genetic engineering. In order to solve the problems that in the prior art, a
phalaenopsis petal color regulation mechanism is not clear, and an effective
molecular breeding target is lacked, through combined analysis of a
transcriptome and a
metabolome, it is found for the first time that the PhCHS-R1
gene is a key regulation factor of a
flavonoid biosynthetic pathway. The functions of the
gene are verified by adopting real-time fluorescent quantitative PCR (
Polymerase Chain Reaction) and LC-MS (Liquid
Chromatography-
Mass Spectrometry) technologies and a transgenic method, experiments show that PhCHS-R1 influences the color of petals by positively regulating and controlling accumulation of
anthocyanin metabolites such as colorless cornflower and
luteolin, and the edition of PhCHS-R1 can cause that the seed coats of
arabidopsis thaliana become white from brown. The invention clarifies the
molecular mechanism of
petal color regulation and control, provides a new target with strong specificity and high
operability for
molecular breeding of
phalaenopsis amabilis, and has important application value for cultivating ornamental varieties with novel flower colors.