Separation and purification method for bilobalide monomers in ginkgo leaf extract
A technology of ginkgo lactone and ginkgo biloba leaves, applied in the direction of organic chemistry and the like, can solve the problems of difficulty in separation and purification of ginkgo lactone, and achieve the effects of high comprehensive utilization value, convenient operation, and efficient separation and selectivity.
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[0021] Example 1: Take 200 grams of Ginkgo biloba extract (containing about 6 grams of ginkgo lactone), add 1600 ml of water and 400 ml of ethyl acetate, and stir to dissolve. Extract 3 times with 1000 ml of ethyl acetate each time, and combine the ethyl acetate extracts. The extract was concentrated to recover ethyl acetate, then concentrated by adding water, and settled overnight to precipitate most of ginkgolide A, ginkgolide B and ginkgolide C. The mother liquor containing ginkgo lactone was adjusted to 5%-10% alcohol, precipitated at room temperature for 2-4 hours, suction filtered, the filtrate was extracted with ethyl acetate petroleum ether mixed solution for 3 times, each 400-600mL, and the light phase was concentrated to dryness, Then, use 300-400 mL of 15%-30% ethanol to dissolve by heating at 60°C, place overnight, and filter. Put the filtrate on a polyamide resin column, the resin volume is 100-200 mL, and 200-400 mL of 10-30% ethanol is decomposed. The effluent ...
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[0022] Example 2: Take 4 kg of Ginkgo biloba extract (containing about 120 grams of ginkgo lactone), add 32 L of water and 8 L of ethyl acetate, and stir to dissolve. Extract 3 times with 20 L of ethyl acetate each time, and combine the ethyl acetate extracts. The extract was concentrated to recover ethyl acetate, then concentrated by adding water, and settled overnight to precipitate most of ginkgolide A, ginkgolide B and ginkgolide C. The mother liquor containing ginkgo lactone was adjusted to 5%-10% ethanol, precipitated at room temperature for 2-4 hours, suction filtered, the filtrate was extracted with ethyl acetate petroleum ether mixed solution for 3 times, each 8-12LL, and the light phase was concentrated to dryness, Then, use 6-8L of 15%-30% ethanol to dissolve by heating at 60°C, place overnight, and filter. Put the filtrate on a polyamide resin column, the resin volume is 2~4L, 4~8L with 10~30% ethanol for analysis, the upper column effluent and the analysis liquid...
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