Extraction process of camel colostrum immune globulin IgA, IgG.

A technology of immunoglobulin and extraction process, which is applied in the field of biochemical products, can solve the problems that the potential value of colostrum is not fully reflected, the effective concentration of active substances is not high enough, and the loss of immune activity is large, and achieves mild conditions and long life. Long, easy-to-operate effect

Inactive Publication Date: 2010-06-23
甘肃省华龙农业开发有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of colostrum resources has just started, mainly some health foods, the effective concentration of active substances in the products is not high enough, its immune activity is greatly lost in the process of processing, and it is also relatively deficient in the comprehensive utilization of resources. Potential value has not been fully reflected

Method used

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  • Extraction process of camel colostrum immune globulin IgA, IgG.
  • Extraction process of camel colostrum immune globulin IgA, IgG.
  • Extraction process of camel colostrum immune globulin IgA, IgG.

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Embodiment Construction

[0044] The technical solutions of the present invention are further described through the following embodiments.

[0045] 1. Extraction of camel colostrum immunoglobulin IgA

[0046] 1. Experimental materials

[0047] Camel colostrum comes from farmers in Zhanggou Village, Suoyang Town, Anxi, Gansu Province.

[0048] DEAE SepHarose (Fast Flow) and Superdex-200 for separation and purification were purchased from PHharmacia.

[0049] Laboratory use 0.06mol / l ZnSO 4 , 1mol / l Na 2 CO 3 , saturated (NH 4 + ) 2 SO 4 , 0.2mol / L PB (pH value 8.0), 0.02mol / L PB (pH value 8.0), 0.3mol / L PB (pH value 8.0), 0.01mol / LPBS (pH value 7.4), gradient PAGE The required reagents were self-prepared.

[0050]The vertical electrophoresis instrument is a product of Hofer Company, the ultraviolet spectrophotometer is a product of Pharmacia Company, and the gel imaging system is a product of Kodak Company.

[0051] 2 Experimental methods

[0052] 2.1 Pretreatment of camel colostrum

[0053...

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Abstract

This invention relates to extraction process of camel colostrum immunoglobulin IgA and IgG, belongs to biochemistic product field. The camel colostrum is the most prolific natural source of immunoglobulin, and is one of the biotic resources that has most concentrated immune factor. This invention supplies a craft of using gradient elution to extract IgA and using ultrafiltration and metal chelatechromatography to extract IgG. By test has show that in product IgA basicly has no other impurity, purity of IgG can reach 97 percent and above.

Description

technical field [0001] The invention relates to the field of biochemical products, especially the deep processing of camel milk. Background technique [0002] Colostrum usually refers to the milk secreted by the female animal within 7 days after delivery, especially within 3 days. Its main characteristics are: yellow and thick color, special milky smell and bitter taste; dry matter content is significantly higher than that of normal milk. The content of protein, albumin and inorganic salts is particularly high, but the content of lactose is lower than that of normal milk; it is rich in vitamins; high in acidity; poor in thermal stability, and clots begin to appear when heated to 60°C. Therefore, the quality standards of dairy products in various countries in the world generally stipulate that the use of colostrum as raw milk is strictly prohibited. A large amount of colostrum in production practice is used to feed newborn cubs, and the surplus part is mostly discarded. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/04
Inventor 王曙阳梁剑平宋楠楠邵伍军华兰英
Owner 甘肃省华龙农业开发有限公司
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