A liquid adding device for immunoblotting

Abstract

The invention discloses a liquid adding device used for immunoblotting. The liquid adding device is provided with one or a plurality of water inlets, and a plurality of water outlets; the water outlets are prepared from a hydrophilic material for absorption of reaction liquid; the water outlets are circular or elliptical, the inner diameter of the water outlets ranges from 0.1 to 2mm, and the outer diameter ranges from 0.5 to 5mm. The liquid adding device can be used for adding reaction liquid onto immunoblotting membrane; the hydrophilic water outlets can be used for absorbing reaction liquid, realizing reciprocating motion of reaction liquid along strip-shaped hydrophilic zones, increasing reaction efficiency thereof, and shortening immunoblotting detection time; the hydrophilic water outlets can be used for absorbing reaction liquid, so that leakage of reaction liquid is avoided, only milliliters or littler reaction liquid is needed for immunoblotting, reagent saving is realized, and the liquid adding device is suitable for trace detection.

Description

technical field [0001] The invention belongs to the field of protein analysis, and more specifically relates to a liquid adding device for immunoblotting. Background technique [0002] Western blotting (Western Blot) is a technique in which proteins are transferred to a membrane and then detected using antibodies. Western blotting is often used to identify a certain protein and can perform qualitative and semi-quantitative analysis of the protein. Combined with chemiluminescence detection, the expression difference of the same protein in multiple samples can be compared at the same time. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay, Western blot has become a routine technique for protein analysis. [0003] In order to realize high-efficiency automated analysis, a variety of Western blot analyzers have emerged as needed (patent document CN103616526A). However, the above-mentioned western blot analyzer usually us...

AI Technical Summary

Problems solved by technology

Therefore, the use of this film has the following disadvantages: 1, because the shaker and the motor for oscillating the reagent bottle are integrated, the structure of the whole analyzer is relatively complicated, thereby increasing the manufacturing cost; 2, the reaction conditions of different reagent bottles are slightly different, Thus affecting the accuracy of western blotting; 3. The film needs to be taken out from the reagent bottle and then dried before detection, so that in-situ detection cannot be realized, resulting in insufficient or over-reaction, resulting in detection errors

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