Rice blast germ LAMP detection primer pairs and application

Abstract

The invention relates to rice blast germ LAMP detection primer pairs and application, and belongs to the field of plant disease prediction research and application in plant protection discipline. The sequence of the primer combination is shown in SEQ ID NO.19,24,31,32; a rice blast germ genome DNA can be efficiently amplified in a specialization mode, and the detection sensitivity of the rice blast germ genome DNA can reach pg level after conditional optimization. The detection method is capable of quickly detecting the carrier rate of rice ears, the time is only about 1h, and the obtained carrier rate of the rice ears by detection is significantly correlated with the severity of late diseases, which indicates that the occurrence and damage of the rice blast can be predicted in real time. According to the results of the detection technology, farmers are guided to accurately use pesticides in real time; the control effect reduction, excessive use and other problems caused by too early or too late pesticide application can be avoided; the detection technology is conducive to protecting the environment, reducing pesticide residues and promoting the sustainable development of agriculture.

Description

technical field [0001] The invention relates to a rapid early warning technology for plant diseases, and belongs to the field of research and application of disease prediction and forecasting in the discipline of plant protection. Background technique [0002] Rice blast is one of the most important diseases in rice producing areas in my country. It can occur throughout the rice growth period and seriously affect the yield and quality of rice. Especially panicle blast often causes a large number of withered and white ears to appear in the field, even failure to harvest. If chemical pesticides cannot be applied timely and effectively, it will not only be difficult to control the occurrence of diseases, but also cause pathogenic bacteria to develop resistance due to the frequent use of chemical pesticides in large quantities, and lead to pesticide residues and environmental pollution. Precise forecasting of diseases is the basis for timely application of pesticides and effect...

AI Technical Summary

Problems solved by technology

If chemical pesticides cannot be applied timely and effectively, it will not only be difficult to control the occurrence of diseases, but also cause pathogenic bacteria to develop resistance due to the frequent use of chemical pesticides in large quantities, and lead to pesticide residues and environmental pollution

Precise forecasting of diseases is the basis for timely application of pesticides and effective disease control. However, the existing methods of disease forecasting mainly rely on experts to systematically conduct large-scale surveys in the field, and then publish disease information based on experience. It also takes time to obtain land information, and with the diversification of cultivation methods, the growth period of crops in each field is not consistent, which leads to the phenomenon of premature or late application of pesticides in production, and the effect of disease prevention is not ideal

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