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Seedling raising method for passion fruit

A technology for passion fruit and seeds, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of decreased plant quality, low reproduction efficiency, etc., and achieves the effects of easy operation, good market prospects, and short cycle times.

Inactive Publication Date: 2017-09-01
李茂兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, passion fruit is mainly propagated by seeds and divisions, the propagation efficiency is low, and it is easy to cause the quality of the plants to decline

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for raising seedlings of passion fruit, soaking the seeds of passion fruit in a potassium permanganate solution with a mass concentration of 1%, and then washing them with sterile water for 3 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the primary medium is MS + sucrose 10 g / L for primary culture, 20°C, humidity 60%, light 2000lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants , and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.02 mg / L + sucrose 10 g / L, 25°C, humidity 70%, light 6000 1x, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthaleneacetic acid 0.5 mg / L + sucrose 20 g / L, 28 ℃, humidity 80%, light 10000 lx...

Embodiment 2

[0022] A method for raising seedlings of passion fruit, soaking the seeds of passion fruit in a potassium permanganate solution with a mass concentration of 3%, and then rinsing them with sterile water for 5 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the primary medium is MS + sucrose 10 g / L for primary culture, 22 ° C, humidity 70%, light 5000 lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings were used as explants body, and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.02 mg / L + sucrose 10 g / L, 28°C, humidity 80%, light 8000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthalene acetic acid 0.5 mg / L+sucrose 20 g / L, 30°C, humidity 90%, light 1...

Embodiment 3

[0024] A method for raising seedlings of passion fruit, soaking the seeds of passion fruit in a potassium permanganate solution with a mass concentration of 2%, and then washing them with sterile water for 4 minutes to obtain sterile seeds; inoculating the sterile seeds into the first generation In the culture medium, the first-generation medium is MS + sucrose 10 g / L, and the first-generation culture is carried out at 25°C, humidity 70%, and light 3000 lx to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants , and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.02 mg / L + sucrose 10 g / L, 28°C, humidity 75%, light 7000 1x, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthaleneacetic acid 0.5 mg / L + sucros...

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PUM

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Abstract

The invention discloses a method for growing seedlings of passion fruit. The seeds of passion fruit are soaked in a potassium permanganate solution with a mass concentration of 1-3%, and then washed with sterile water to obtain sterile seeds; Bacteria seeds are inoculated in the primary culture medium, and the primary culture is carried out to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants, and inoculated in the secondary culture medium, and the subculture is carried out to obtain Rootless seedling: described rootless seedling is transplanted in the rooting medium, carries out rooting culture, obtains passion fruit seedling. The method for growing seedlings by tissue culture of the invention has high rooting efficiency and high survival rate, and can maintain good quality of plants.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and seedling cultivation, and in particular relates to a method for cultivating passion fruit seedlings. Background technique [0002] Passion fruit has anti-inflammatory and analgesic effects, promoting blood circulation and strengthening the body, reducing fat and blood pressure, nourishing yin and tonifying kidney, refreshing and sobering up, eliminating fatigue, detoxifying and beautifying, and enhancing immunity. The mechanism of action is as follows: 1. Deep cleaning mechanism: superfiber can penetrate The tiniest part of the intestines and stomach, through its active genes, absorbs harmful substances in the body and completely discharges them, and can improve the composition of the intestinal flora, acting as a barrier to protect the intestines and stomach from absorbing harmful substances. Passion fruit can inhibit the growth of harmful microorganisms in the digestive tract. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李茂兰蒋军
Owner 李茂兰
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