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Method for rapidly detecting chemical component tadalafil and derivatives thereof and method for developing detection card

A technology of chemical composition and derivatives, which is applied in the direction of analyzing materials through chemical reactions, analyzing materials through observation of the influence of chemical indicators, and analyzing materials through optical means. It can solve the problems of high professional quality requirements, Problems such as long time-consuming and high detection cost, to achieve the effect of high professional quality requirements, long detection cycle and high detection cost

Inactive Publication Date: 2017-09-08
北京倍肯恒业科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the existing rapid detection methods for health food, functional medicines, Lafia and its derivatives in food, false detection and missed detection, only qualitative detection; Due to the high professional quality requirements of personnel and the complicated and cumbersome pretreatment of samples, the present invention provides a rapid detection method and a detection card development method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Rapid detection method of ralafidine and its derivatives in tablet, soft capsule, and hard capsule health food

[0030] The detection process is as follows:

[0031] 1) Pretreatment of the sample to be tested: Weigh 0.10 g of the sample, put it into a 2ml centrifuge tube, add 0.90 ml of the extract, mix well, let it stand for 5 minutes, take the supernatant and filter it with a microporous membrane, and the filtrate is the sample to be tested Sample treatment solution;

[0032] 2) Detection: Take 100 microliters of the sample treatment solution to be tested and add it to a 1.5ml centrifuge tube, add 400 microliters of the detection solution and mix well, let it stand for 15 minutes for color development, after mixing, take 50 microliters and transfer it to the observation window of the test card In the middle position, read the test result on the instrument after timing for one minute.

Embodiment 2

[0033] Example 2: Rapid detection method of ralafidine and its derivatives in liquid alcohol or oral liquid health food

[0034] The detection process is as follows:

[0035] 1) Pretreatment of the sample to be tested: Measure 0.10 ml of the sample, put it into a 2 ml centrifuge tube, add 0.90 ml of the extract, mix well, let it stand for 5 minutes, and take the colorless liquid layer as the sample treatment solution for the test.

[0036] 2) Detection: Take 100 microliters of the sample treatment solution to be tested and add it to a 1.5ml centrifuge tube, add 400 microliters of the detection solution and mix well, let it stand for 15 minutes for color development, after mixing, take 50 microliters and transfer it to the observation window of the test card In the middle position, read the test result on the instrument after timing for one minute.

Embodiment 3

[0037] Embodiment 3: rapid detection method of latidine and its derivatives in medicines

[0038] The detection process is as follows:

[0039] 1) Pretreatment of the sample to be tested: Weigh 0.10 g of crushed medicine, put it into a 2ml centrifuge tube, add 0.90 ml of extract, mix well, let stand for 5 minutes, take the supernatant and filter it with a microporous membrane, The filtrate is the sample processing liquid to be tested;

[0040] 2) Detection: Take 100 microliters of the sample treatment solution to be tested and add it to a 1.5ml centrifuge tube, add 400 microliters of the detection solution and mix well, let it stand for 15 minutes for color development, after mixing, take 50 microliters and transfer it to the observation window of the test card In the middle position, read the test result on the instrument after timing for one minute.

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PUM

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Abstract

The invention discloses a method for rapidly detecting a chemical component tadalafil and derivatives thereof and a method for developing a detection card. The detection method comprises the following steps: firstly, soaking a to-be-detected sample with an extracting solution, fully and uniformly mixing and standing; secondly, filtering the supernatant by using a microfiltration membrane, wherein the filtrate is a treatment solution of the to-be-detected sample; thirdly, taking a certain amount of the filtrate, adding a detection solution in a volume of 4 times for uniformly mixing, standing and developing; fourthly, transferring 50microliters of mixed solution to a middle position of a detection card observation window, timing for a minute, and reading the detection result on an instrument. The method for developing a detection card comprises the following steps: preparing a test paper base material, preparing a soaking solution, soaking the test paper base material, preparing detection test paper and preparing the detection card. The method is suitable for rapid field detection of the chemical component tadalafil and derivatives thereof in foods, drugs and health care products.

Description

technical field [0001] The invention belongs to the technical field of detection of food, medicine and health food, and in particular relates to a method for rapid detection of the chemical component ralphetamine and its derivatives in food, medicine and health food and a method for developing a detection card. Background technique [0002] 1. Terminology explanation: [0003] Hazardous factors in food and illegally added drugs intelligent comprehensive detector: referred to as the detector, also known as the handheld food safety rapid detector, using the principle that the reflectance spectrum and the chromogenic substance are proportional to the concentration of the detected components, calculated by the internal formula An instrument that obtains a numerical value for the concentration of a component to be detected. [0004] Laffil and its derivatives specifically refer to: tadalafil, aminotadalafil, and compounds whose methyl group is substituted by amino, C1-6 alkyl, C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 姚世平刘光中姚洪涛周轶李心乐刘春秀
Owner 北京倍肯恒业科技发展股份有限公司
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