Molecular marker closely linked with rape plant height main effect QTL (quantitative trait locus) qPHC2 and application

A technology of molecular markers and rape, applied in the fields of molecular biology and genetic breeding

Active Publication Date: 2017-09-15
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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However, the cloning of genes related to plan

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  • Molecular marker closely linked with rape plant height main effect QTL (quantitative trait locus) qPHC2 and application
  • Molecular marker closely linked with rape plant height main effect QTL (quantitative trait locus) qPHC2 and application
  • Molecular marker closely linked with rape plant height main effect QTL (quantitative trait locus) qPHC2 and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Acquisition of main QTL locus qPH.C2 for plant height traits in rapeseed:

[0028]1) The segregated populations adopted in the present invention are the F2, F2:3 and F2:4 family populations derived from the parent Zhongshuang 11 and No.73290. The plant height phenotypes of the two parents and the F2:3 and F2:4 two-line populations were identified after harvesting at the mature stage. The investigation data of plant height traits showed that there was super-parent segregation phenomenon of plant height traits ( figure 1 ).

[0029] 2) Using the F2 (BnaZNF2) population of 184 individual plants that were heterozygous genotypes selected from the offspring of Zhongshuang 11 and No.73290 as the analysis population, the BnaZNF 2 Genotype analysis of the population, using 9531 primers evenly distributed in 19 chromosomes, including 6504 SSR primers, 2592 SNP markers, 179 STS markers, 76 InDel markers, of which 1038 primers were between two parents Specific polymorphisms exis...

Embodiment 2

[0039] Verification of the validity of SSR markers BoGMS0665 and CNU461

[0040] The F2 population was grouped according to the genotype sources of BoGMS0665 and CNU461 markers, and non-recombinant individual plants were selected for subsequent comparative analysis (Table 3). Then, statistical comparative analysis was performed on the plant height phenotypes of the F2:3 and F2:4 populations corresponding to these individual plants to determine whether the differences between different categories were significant.

[0041] Use the F1 obtained by crossing Shuang11 and No.73290 in the parent to continue planting to obtain F2 single plants, and select the genotype dominant heterozygous for planting F2:3;

[0042] Sampling was carried out before the F2:3 single plant was established, and the total DNA of the leaves was extracted. The main QTL genotype of the plant height trait was analyzed by using SSR markers BoGMS0665 and CNU461, and the genotype was judged according to the band ...

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Abstract

The invention discloses a molecular marker closely linked with a rape plant height main effect QTL (quantitative trait locus) qPHC2 and application. F2 group and groups derived from the same are built through double 11 and No.73290 in parents to acquire the main effect gene locus qPHC2 for controlling rape plant height on a C2 linage group, and the locus is closely linked with SSR markers BoGMS0665 and CNU461. F2:3 and F2:4 groups are utilized to verify effectiveness of molecular markers BoGMS0665 and CNU461. A novel effective molecular marker is provided for genetic improvement of rape plant height, and useful information is provided for fine locating and gene cloning of the rape plant height main effect QTL qPHC2.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and specifically relates to a major gene locus for plant height traits of Brassica napus and two molecular markers closely linked to it, and also relates to the gene locus and molecular markers in rapeseed plant type application in breeding. Background technique [0002] Rapeseed is the most important oil crop in my country and the only winter oil crop in China (Hu, 2016). Its planting area and total output account for about one-third of the world (James, 2003). Rapeseed oil is the second largest source of vegetable oil in the world, and it is also the largest source of edible vegetable oil in my country, accounting for more than 55% of the oil production of domestic oil crops in my country. It plays a very important role in the national edible oil supply security strategy (Wang Hanzhong, 2014). In recent years, the annual output of domestic vegetable oil in my cou...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王汉中师家勤张江江詹杰鹏刘贵华王新发
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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