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Method for quickly measuring content of bacteria in sewage of oil field and finished oil

A technology for oilfield sewage and refined oil, applied in biochemical equipment and methods, microbial measurement/inspection, specific-purpose bioreactors/fermentation tanks, etc., can solve problems such as inconvenience, inaccurate results, and slow measurement speed

Active Publication Date: 2017-09-29
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is the problem of slow measurement speed, inconvenient and inaccurate results in the prior art, and a new method for rapidly measuring the bacterial content in oilfield sewage and refined oil is provided

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  • Method for quickly measuring content of bacteria in sewage of oil field and finished oil
  • Method for quickly measuring content of bacteria in sewage of oil field and finished oil

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Embodiment 1

[0029] A method for rapidly measuring the bacterial content of oilfield sewage and refined oil, such as figure 1 , figure 2 shown. The bacteria content measurement device in the liquid sample is used to measure the bacteria content in the liquid sample; the device includes a box, bacteria enrichment and nucleic acid molecule extraction equipment, and the bacteria enrichment and nucleic acid molecule extraction equipment includes a reagent tube placement position (1) and reagent tube placement position (4), filter membrane (2), adsorption layer (3) reaction pool (5), light source (6), optical filter (7), photoreceptor (8) and at least four Valve, the bottom of the reagent tube placement position (1) communicates with the space above the filter membrane (2), and the space below the filter membrane (2) is divided into two paths, one path is connected to the inlet end of valve a, and the other path is connected to the inlet end of valve b , the outlet end of valve a is connecte...

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Abstract

The invention relates to a method for quickly measuring content of bacteria in sewage of an oil field and finished oil, which mainly aims at solving the problems of lower measuring speed, inconvenience and inaccurate results in the prior art. The method for quickly measuring the content of bacteria in the sewage of the oil field and finished oil comprises a box body and bacteria enriching and nucleic acid molecule extracting equipment, wherein the bacteria enriching and nucleic acid molecule extracting equipment comprises a reagent tube placing position (1), a reagent tube placing position (4), a filtering membrane (2), an adsorbing layer (3), a reaction tank (5), a light source (6), a light filter (7), a light sensor (8) and at least four valves. By adopting the technical scheme, the method has the advantages that the technical problems are well solved, and the method can be used for measuring the content of bacteria in a liquid sample.

Description

technical field [0001] The invention relates to a method for quickly measuring the bacteria content in oil field sewage and refined oil. Background technique [0002] The corrosion damage of materials caused by the life activities of microorganisms such as bacteria is called microbial corrosion (MIC). MIC is a ubiquitous phenomenon. There are a lot of bacteria in oil and gas fields, oil tanks, gas stations, oil lines and even automobile fuel tanks. The economic loss caused by corrosion is more than 10 billion U.S. dollars every year. Among them, the most important corrosion microorganisms are sulfate reducing bacteria (SRB), which caused more than 77% of the corrosion loss of American production oil wells. SRB can use the organic matter in the environment as the carbon source in an anaerobic environment, use the hydrogen produced in the bacterial biofilm, reduce sulfate to hydrogen sulfide, and obtain energy from the redox reaction, and the metal is also destroyed in this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/06C12M1/36C12M1/34
CPCC12Q1/6806C12Q1/6851C12Q2523/308C12Q2563/107C12Q2565/607
Inventor 孙冰王林徐伟文松
Owner CHINA PETROLEUM & CHEM CORP
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