A kind of aquatic biological source cross-linked collagen composite multi-layer medical dressing

A technology of collagen and aquatic organisms, applied in the field of medical materials, can solve the problems of natural collagen dressings such as poor mechanical strength, weak resistance to degradation, and wounds susceptible to microbial infection, achieve excellent hemostatic effect, resist bacterial invasion, and improve comprehensive The effect of utilization

Active Publication Date: 2021-01-26
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of aquatic biological source cross-linked collagen composite multi-layer medical dressing, so as to solve the problems of poor mechanical strength of natural collagen dressing, weak anti-degradation ability, and wound susceptibility to microbial infection

Method used

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  • A kind of aquatic biological source cross-linked collagen composite multi-layer medical dressing
  • A kind of aquatic biological source cross-linked collagen composite multi-layer medical dressing
  • A kind of aquatic biological source cross-linked collagen composite multi-layer medical dressing

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Effect test

Embodiment 1

[0038]The preparation method of aquatic biological source cross-linked collagen composite multilayer medical dressing is as follows:

[0039](1) Preparation of high-purity and high-activity medical collagen: Tilapia skin is scraped off fish scales, residual meat and fat with a blade, washed in cold water, and cut into 0.5–1cm with a slicer2Of small pieces. The fish skin is treated with ultra-high pressure to increase the extraction rate of collagen, the pressure is 200 MPa, and the treatment time is 5 min. Soak the fish skin in 2% Triton X-100 phosphate buffer, place it on a shaker for 48h, 100rpm, 4℃, shake and rinse in distilled water, and then soak the fish skin in 1% SDS phosphate buffer Place it on a shaker for 48h, 100rpm, 4°C, shake and rinse in distilled water, repeat the above steps 3 times to remove cells from the tissue. Add 0.1M NaOH solution (1:20, w / v) and soak for 24h to remove non-collagen components and pigments. Wash with distilled water repeatedly until it is neutral...

Embodiment 2

[0057]The preparation method of aquatic biological source cross-linked collagen composite multilayer medical dressing is as follows:

[0058](1) Preparation of high-purity and high-activity medical collagen: use a blade to scrape off the scales, residual meat and fat of cod skin, wash in cold water, and cut into 0.5–1cm with a slicer2Of small pieces. The fish skin is treated with ultra-high pressure to increase the extraction rate of collagen, the pressure is 150 MPa, and the treatment time is 10 min. Soak the fish skin in 1% Triton X-100 phosphate buffer, place it on a shaker for 48h, 120rpm, 4℃, shake and rinse in distilled water, and then soak the fish skin in 1% SDS phosphate buffer Place it on a shaker for 48h, 120rpm, 4°C, shake and rinse in distilled water, repeat the above steps 3 times to remove cells in the tissue. Add 0.1M NaOH solution (1:20, w / v) and soak for 24h to remove non-collagen components and pigments. Wash with distilled water repeatedly until it is neutral. Add 1...

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Abstract

The invention discloses a high-purity and high-activity medical collagen of aquatic biological source, which solves the problems of poor mechanical strength, weak anti-degradation ability and easy microbial infection of natural collagen dressings. Collagen is extracted by acid extraction combined with pepsin to remove telopeptides, and the extraction rate is improved by ultra-high pressure treatment and tissue crushing, and through decellularization, impurity removal, degreasing, multiple salting out, dialysis, and sterilization , decolorization and depyrogenation and other series of purification operations to prepare high-purity and high-activity medical collagen. The network-like collagen sponge with uniform pores was prepared by vacuum freeze-drying process, and then the collagen sponge was modified by physical cross-linking and chemical cross-linking to prepare the inner layer, and then combined with chitosan outer film and medical non-woven The cloth-based cloth layer is compounded, and finally the finished composite multi-layer medical dressing is obtained after Co-60 sterilization.

Description

Technical field[0001]The invention belongs to the technical field of medical materials, and specifically relates to a multi-layer medical dressing for aquatic biological source cross-linked collagen composite.Background technique[0002]Collagen is a kind of natural degradable biological macromolecule with wide sources, low immunogenicity and good biocompatibility. It has excellent hemostatic properties, has a good hemostatic effect on patients with coagulation dysfunction, and can promote granulation The regeneration and repair of tissues can promote wound healing. Over the years, clinical practice has shown that traditional physical, chemical, and mechanical hemostasis methods have certain limitations and adverse reactions, and natural polymer dressings such as collagen are an effective surgical hemostasis method. Collagen can be applied to the hemostasis of various solid organs, body surface and bone tissue, blood vessel tissue and connective tissue. American IMS company conducted ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/00A61L24/10A61L15/42A61L15/32A61L15/28A61L15/46A61L15/44A61L15/24C12P21/02C07K1/34C08J9/28C08J7/12C08J7/00C08L89/00
CPCA61L15/24A61L15/28A61L15/325A61L15/425A61L15/44A61L15/46A61L24/0036A61L24/102A61L2300/232A61L2300/252A61L2300/404A61L2300/412C07K14/78C08J7/08C08J7/12C08J7/123C08J9/28C08J2201/0484C08J2389/00C12P21/02C08L5/08C08L33/08
Inventor 李八方侯虎孙蕾蕾张朝辉赵雪
Owner OCEAN UNIV OF CHINA
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