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Method for improving drought resistance of citruses

A drought-resistant, citrus technology, applied in the fields of botanical equipment and methods, chemicals for biological control, agricultural gas emission reduction, etc., to achieve the effect of promoting vegetative growth, low production cost, and improving the stability of aggregates

Inactive Publication Date: 2017-10-13
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The role of exogenous glomycin in improving the drought resistance of citrus by arbuscular mycorrhizal fungi has not been reported yet

Method used

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  • Method for improving drought resistance of citruses
  • Method for improving drought resistance of citruses
  • Method for improving drought resistance of citruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of glomycin protein drought-resistant agent

[0028] Collect the yellow soil with soil depth of 0-20cm from the orange orchard. This embodiment adopts the 0-20cm soil (yellow soil) collected in March, 2016 in the 26-year-old citrus orchard of Changjiang University, Jingzhou City. Sieve to remove roots, litter, and rocks from the soil. Then repeatedly extract a sufficient amount of easy-to-extract glomycin solution according to the following method:

[0029] Weigh 5.0g of the treated soil and put it into a centrifuge tube, add 40mL of 20mmol / L (pH=7.0) sodium citrate extract, then shake on the shaker for 60s, high temperature sterilization pot 103kpa, 121℃ Extract for 30 minutes, centrifuge at 10,000 g / s for 10 minutes, collect the supernatant to obtain an easily extractable glomycin solution, and measure the concentration of the easily extractable glomycin in the soil to be 0.014 mg / mL after the extraction is completed. Finally, use the complete easy-to...

Embodiment 2

[0071] This embodiment provides a kind of anti-drought agent that improves the drought resistance of citrus, and its preparation step comprises:

[0072] S1. Collect 0-20cm yellow soil in the orangery, mix evenly, air-dry, pass through a 4mm sieve to remove impurities such as roots, garbage and stones in the soil;

[0073] S2, prepare 20mmol / L (pH=7.0) sodium citrate extract: (1) A solution: 0.02mol / L citric acid solution (take 4.2g C 6 h 8 o 7 ·H 2 O dissolved in 1000mL distilled water); (2) B solution: 0.02mol / L disodium hydrogen phosphate solution (take 5.365gNa 2 HPO 4 ·7H 2 O or 7.16g Na 2 HPO 4 12H 2 O dissolved to 1000mL); (3) Measure 130mL and 870mL of A liquid and B liquid respectively, mix well to obtain 1000mL 20mmol / L, pH=7.0 sodium citrate buffer.

[0074] Take 4.0g of the soil obtained in step S1 and put it into a 50mL centrifuge tube, add 32mL of 20mmol / L, pH=7.0 sodium citrate buffer, shake for 60s, then extract at 103kpa, 121°C for 30min, and then ext...

Embodiment 3

[0077] This embodiment provides a kind of anti-drought agent that improves the drought resistance of citrus, and its preparation step comprises:

[0078] S1. Collect 0-20cm yellow soil in the orangery, mix evenly, air-dry, pass through a 4mm sieve to remove impurities such as roots, garbage and stones in the soil;

[0079] S2, prepare 20mmol / L (pH=7.0) sodium citrate extract: (1) A solution: 0.02mol / L citric acid solution (take 4.2g C 6 h 8 o 7 ·H 2 O dissolved in 1000mL distilled water); (2) B solution: 0.02mol / L disodium hydrogen phosphate solution (take 5.365gNa 2 HPO 4 ·7H 2 O or 7.16g Na 2 HPO 4 12H 2 O dissolved to 1000mL); (3) Measure 130mL and 870mL of A liquid and B liquid respectively, mix well to obtain 1000mL 20mmol / L, pH=7.0 sodium citrate buffer.

[0080] Take 5.0g of the soil obtained in step S1 and put it into a 50mL centrifuge tube, add 32mL of 20mmol / L, pH=7.0 sodium citrate buffer, shake for 60s, then extract at 103kpa, 121°C for 30min, and then extra...

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Abstract

The invention provides a method for improving the drought resistance of citruses. The method comprises the step of applying a drought-resistant agent to the root circumference of citrus plants, wherein active ingredients of the drought-resistant agent include glomalin. The invention further provides ingredients and specific preparation steps of the drought-resistant agent. Through the adoption of the method for improving the drought resistance of the citruses, the effect of improving the drought resistance of the citruses is significant, the vegetative growth of the citrus plants is promoted, and the biomass is increased; the development of root configuration is promoted, and the length of main roots and the number of lateral roots at all levels are increased; the photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO2 concentration of leaves are increased, and the temperature of the leaves is reduced; the relative water content of the leaves is increased, and the water potential of the leaves is reduced; the content of water stable aggregates in rhizosphere soil is increased, and the stability of the water stable aggregates is improved; the activity of antioxidant enzymes in plant leaves and roots is improved; the contents of abscisic acid, methyl jasmonate and auxin in the leaves and roots are increased. The method for improving the drought resistance of the citruses has the advantages that raw materials are easy to obtain, the production cost is low, the method is low in toxicity, efficienct, environmentally friendly, low in consumption and the like.

Description

technical field [0001] The invention relates to the technical field of plant resistance improvement, in particular to a method for improving the drought resistance of citrus. Background technique [0002] Glomuscin is the only glycoprotein secreted into soil by arbuscular mycorrhizal fungi found so far. The existence of glomamycin in soil is very abundant, which can be divided into total glomamycin, easy-extractable glomamycin, immunoreactive total glomamycin, and immunoreactive easy-extractable glomamycin. Due to the specificity of the composition and properties of glomycin, it is of great significance in soil structure and soil organic matter composition. [0003] Drought, the loss of soil moisture due to prolonged periods of insufficient rainfall, is one of the most severe types of natural disasters in the world. Worldwide, the losses caused by drought and water shortage to agriculture and society are equivalent to the sum of other natural disasters. Citrus is one of t...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01N63/04A01P21/00
CPCA01G17/005A01N63/30Y02P60/20
Inventor 吴强盛邹英宁池格格刘春艳
Owner YANGTZE UNIVERSITY
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