New uses of GP73 and liver tissue inflammatory activity detection kit based thereon

A detection kit, liver tissue technology, applied in the field of biomedicine, to achieve the effect of efficient treatment, efficient diagnosis, and rapid wound recovery

Active Publication Date: 2017-10-13
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no suitable markers have been found to indicate the level and grade of liver tissue inflammation activity
[0009] At present, there is no sensitive and efficient method for detecting liver tissue inflammation activity by serum rather than by tissue

Method used

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  • New uses of GP73 and liver tissue inflammatory activity detection kit based thereon
  • New uses of GP73 and liver tissue inflammatory activity detection kit based thereon
  • New uses of GP73 and liver tissue inflammatory activity detection kit based thereon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1 experimental design

[0044] 1.1 Research object

[0045] From December 2013 to December 2016, 642 patients with chronic liver disease underwent B-ultrasound-guided liver puncture in Beijing 302 Hospital.

[0046] 1.2 Statistical methods

[0047] The SPSS21.0 (SPSS, Chicago, Illinois, USA) statistical software was used for statistical analysis, the continuous variable was selected according to its distribution characteristics, and the quantitative data approximately obeying the normal distribution was used x ±S means that the median (M) and interquartile range are used to describe the distribution characteristics of the quantitative data showing a skewed distribution, the χ2 test is used for the comparison between qualitative data groups, and the Mann-WhitnyU test is used for the comparison between two independent samples. The Kruskal-Wallis test was used between samples. Receiver operating characteristic curve (ROC curve) was established, and the eval...

Embodiment 2

[0054] Embodiment 2 test result analysis

[0055] 2.1 Expression levels of serum GP73, ALT, AST in different degrees of liver tissue inflammation activity

[0056] The change trend of serum GP73, ALT and AST in different degrees of liver tissue inflammation activity was described by interquartile range. The levels of serum GP73 in different degrees of liver tissue inflammation activity were G0-1 ​​(n=241): 41.43 (24.34-58.08) ng / mL; G2 (n=239): 69.26 (45.66-115.38) ng / mL; G3 / 4 (n=46): 122.54 (72.19-186.51) ng / mL; Spearman correlation analysis showed that serum GP73 was significantly correlated with liver tissue inflammation activity (Spearman correlation coefficient r=0.609, Pfigure 1 and Table 3 below.

[0057] Table 3 Interquartile ranges of GP73, ALT, AST in patients with different liver tissue inflammation activities

[0058]

[0059]

[0060] 2.2 The distinguishing value of serum GP73 on the activity of liver tissue inflammation

[0061] Using the pathological ...

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PUM

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Abstract

The invention relates to use of serum GP73 concentration in liver tissue inflammatory activity detection, and provides a liver tissue inflammatory activity detection kit. The kit utilizes the GP73 concentration in serum to realize detection and grading of subjects' liver tissue inflammatory activity, and is suitable for patients suffering moderate inflammation necrosis or above and having normal ALT, and viral hepatitis patients adopting antiviral therapy.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a new application of Golgi protein 73 (GP73) and a detection kit for liver tissue inflammation activity prepared based on detecting the concentration of Golgi protein 73 (GP73). Background technique [0002] Kladney and others discovered Golgi protein 73 (GP73) in the study of giant cell hepatitis in adults, and it has been 17 years since then (Kladney RD, Bulla GA, Guo L, et al.Gene.2000.249(1-2):53-65) GP73 is a transmembrane glycoprotein with 402 amino acids and a relative molecular weight of 73kDa, also known as type II Golgi membrane protein (Golgiphosphoprotein 2, Golph2) and Golgi membrane protein 1 (Golgi membrane protein 1, Golm1). It is usually located in the trans-Golgi complex. The gene encoding GP73 protein is located on chromosome 9, with a total length of 3080 nucleotides, and the coding region is located at 199-1404nt. GP73 is expressed in various tissues, but its strengt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/576G01N2800/085
Inventor 鲁凤民姚明解
Owner PEKING UNIV
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