Application of Polycarpine Salts in Anti-Plant Viruses and Germs

A technology against plant viruses and plant pathogens, applied in the directions of botanical equipment and methods, applications, plant growth regulators, etc.

Active Publication Date: 2019-09-27
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] So far, the research on alkaloid Polycarpine and its derivatives in the control of plant viruses and bacteria is still blank

Method used

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  • Application of Polycarpine Salts in Anti-Plant Viruses and Germs
  • Application of Polycarpine Salts in Anti-Plant Viruses and Germs
  • Application of Polycarpine Salts in Anti-Plant Viruses and Germs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: The experimental data of Polycarpine salt I-1~I-7

[0027] Polycarpine hydrochloride (I-1): yellow solid, melting point 209-211°C, 1 H NMR (400MHz, DMSO-d 6 )δ13.42(s, 2H, HCl), 7.66(s, 4H, NH 2 ), 7.45(d, J=8.6Hz, 4H, Ar-H), 7.00(d, J=8.6Hz, 4H, Ar-H), 3.89(s, 6H, O-CH 3 ), 3.18 (s, 6H, N-CH 3 ). 13 C NMR (100MHz, DMSO-d 6 )δ160.6, 147.3, 136.9, 127.5, 117.1, 114.1, 108.8, 55.4, 28.7. HRMS (ESI) calcd for C 22 h 25 N 6 o 2 S 2 + [M+H-2HCl] + 469.1475, found 469.1469.

[0028] Polycarpine ferulate (I-2): dark red solid, melting point 136-138°C, 1 H NMR (400MHz, DMSO-d 6 )δ12.15 (brs, 2H, COOH), 9.59 (brs, 2H, OH), 7.54 (brs, 4H, NH 2 ), 7.49 (d, J=15.9Hz, 2H, CH), 7.28 (s, 2H, Ar-H), 7.08 (d, J=8.0Hz, 2H, Ar-H), 6.83 (brs, 4H, Ar-H -H), 6.79(d, J=8.0Hz, 2H, Ar-H), 6.37(d, J=15.9Hz, 2H, CH), 6.13(brs, 4H, Ar-H), 3.81(s, 6H , O-CH 3 ), 3.75 (brs, 6H, O-CH 3 ), 3.17 (brs, 6H, N-CH 3 ). 13 C NMR (100MHz, DMSO-d 6 )δ168.7, 149.5, 148.4, 14...

Embodiment 2

[0034] Embodiment 2: the assay of anti-tobacco mosaic virus activity, assay procedure is as follows:

[0035] 1. Virus purification and concentration determination:

[0036] Virus purification and concentration determination were carried out in accordance with the SOP specification for tobacco mosaic virus compiled by the Bioassay Laboratory of the Institute of Elements, Nankai University. After the crude virus extract was centrifuged twice with polyethylene glycol, the concentration was measured and refrigerated at 4°C for later use.

[0037] 2. Compound solution preparation:

[0038] After weighing, the original drug was dissolved in DMF to prepare 1×10 5 μg / mL mother solution, and then diluted with 1‰ Tween 80 aqueous solution to the required concentration; Ningnanmycin preparation was directly diluted with water.

[0039] 3. In vitro effect:

[0040] Rub inoculation of leaves of Shanxi tobacco at the right age, rinse with running water, the virus concentration is 10 μg...

Embodiment 3

[0051] Embodiment 3: antibacterial activity test, assay procedure is as follows:

[0052] A. In vitro bactericidal test, bacterial growth rate determination method (plate method):

[0053] Dissolve a certain amount of medicine in an appropriate amount of acetone, then dilute it with an aqueous solution containing 200ug / mL emulsifier to the required concentration, then draw 1mL of the medicine solution into the petri dish, then add 9mL of medium, shake well and make 50ug / mL mL of the drug-containing plate, and a plate with 1 mL of sterilized water added as a blank control. Use a puncher with a diameter of 4mm to cut out the bacterial plate along the outer edge of the hyphae, and move it to the drug-containing plate. Each treatment was repeated three times. Place the culture dish in a constant temperature incubator at 24±1°C. After 48 hours, investigate the expanded diameters of the bacteria discs of each treatment, calculate the average value, and compare with the blank cont...

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PUM

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Abstract

The invention relates to an application of Polycarpine salt in anti-plant viruses and pathogenic bacteria. A meaning of X in the formula is as shown in the specification. The Polycarpine salt in the invention shows particularly excellent anti-plant virus activity, and is capable of well inhibiting tobacco mosaic virus (TMV). The compounds simultaneously show excellent plant pathogenic bacteria resistant activities.

Description

technical field [0001] The invention relates to the application of Polycarpine salt in resisting plant viruses and germs, and belongs to the technical field of agricultural protection. Background technique [0002] The ocean is a natural treasure house of human material resources. The total number of species of known marine organisms accounts for more than 80% of the earth's organisms (J Antibiot (Tokyo), 1994, 47, 1425-1433). More than 7,000 kinds of marine natural products have been isolated from sharks, corals and other marine organisms, and the newly discovered compounds are still increasing at an accelerated rate. Among the discovered compounds include terpenoids, polypeptides, steroids, polyethers, alkaloids, macrolides, polysaccharides and other compounds, about 50% have various biological activities, and more than 0.1% of the compounds have novel structures. It has remarkable activity and is very likely to be developed into a drug (China New Drug Impurities, 2005, 1...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/50A01P1/00A01P3/00
Inventor 汪清民郭朋滨王兹稳刘玉秀
Owner NANKAI UNIV
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