Preparation method of animal antihuman serum based on accurate control of immunization sites

Abstract

The invention discloses a preparation method of an animal antihuman serum based on accurate control of immunization sites. First-time inoculation is carried out on 22 sites including a site on each hindfoot pit, a site on each of a left submaxillary lymph node and a right submaxillary lymph node, two sites on the periphery of each armpit, three sites on the periphery of each femoral lymph node and four sites of each of the left side and the right side of a back vertebral column. From second-time injection to last-time injection, injection is carried out on 20 sites, and sites on the hindfoot pits are omitted. The disadvantage of unclean description on multi-site immunity reported on all the original textbooks and data is overcome; the preparation method is accurate, and is easy to learn, master and control; the use amount of antigen is reduced, and the immunity cost is greatly reduced; all immune response of the body can be transferred fully, and the antigen which is difficult to immunize has good antibody titer; individual difference of various sheep can be overcome, the antibody titer is uniform, and stable raw materials can be provided for the IVD industry; large-scale production of the biotechnology on preparation of animal antibodies become possible; and the use amount of the antigens can be reduced by 20% or above.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for preparing animal anti-human serum based on precise control of immune sites. Background technique [0002] Antiserum is serum containing a certain type of antibody molecule with specific immune function, generally animal serum prepared after animals are artificially injected with a certain type of antigen. Whether an antigen can cause antibody production depends on the one hand whether there is an antigenic determinant on the surface of the antigen molecule, and on the other hand depends on the immune status of the body. When the above two conditions are met, antibody production will follow the antibody General law of generation - first reaction and second reaction. There are many kinds of antigens, including natural protein antigens and cellular antigens, synthetic antigens and genetic engineering antigens, etc. Different antigens have different specificiti...

AI Technical Summary

Problems solved by technology

[0004] At present, when sheep anti-human serum is produced, the site of each immunization injection is different, and multi-point injections are mainly performed subcutaneously on the plantar, groin, armpit, neck, and back of the animal, with 1-2 points for each site, totaling From 10 to 16 o'clock, the site of each immunization injection is different, which will lead to an increase in the amount of antigen used and a higher cost, and there are individual differences among sheep, so that the antibody titer cannot be uniform

Images